ID   FIXJ_BRADU              Reviewed;         205 AA.
AC   P23221;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Transcriptional regulatory protein FixJ;
GN   Name=fixJ; OrderedLocusNames=bll2759;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=2000090; DOI=10.1007/bf00282640;
RA   Anthamatten D., Hennecke H.;
RT   "The regulatory status of the fixL- and fixJ-like genes in Bradyrhizobium
RT   japonicum may be different from that in Rhizobium meliloti.";
RL   Mol. Gen. Genet. 225:38-48(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: FixJ, when activated by FixL, induces the expression of both
CC       nifA, required for activation of classical nif and fix genes, and fixK,
CC       required for FixN activation.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by FixL. {ECO:0000305}.
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DR   EMBL; X56808; CAA40144.1; -; Genomic_DNA.
DR   EMBL; AJ005001; CAA06275.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC48024.1; -; Genomic_DNA.
DR   PIR; S13331; S13331.
DR   RefSeq; NP_769399.1; NC_004463.1.
DR   RefSeq; WP_011085544.1; NZ_CP011360.1.
DR   AlphaFoldDB; P23221; -.
DR   SMR; P23221; -.
DR   STRING; 224911.27351016; -.
DR   EnsemblBacteria; BAC48024; BAC48024; BAC48024.
DR   GeneID; 64022516; -.
DR   KEGG; bja:bll2759; -.
DR   PATRIC; fig|224911.44.peg.2380; -.
DR   eggNOG; COG4566; Bacteria.
DR   HOGENOM; CLU_000445_90_4_5; -.
DR   InParanoid; P23221; -.
DR   OMA; KMNKHIA; -.
DR   PhylomeDB; P23221; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS00622; HTH_LUXR_1; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Magnesium; Metal-binding;
KW   Nitrogen fixation; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..205
FT                   /note="Transcriptional regulatory protein FixJ"
FT                   /id="PRO_0000081101"
FT   DOMAIN          6..120
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          136..201
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        160..179
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   BINDING         11
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         12
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         55
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         57
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         55
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   205 AA;  22318 MW;  F2A63A1BDB199172 CRC64;
     MTTKGHIYVI DDDAAMRDSL NFLLDSAGFG VTLFDDAQAF LDALPGLSFG CVVSDVRMPG
     LDGIELLKRM KAQQSPFPIL IMTGHGDVPL AVEAMKLGAV DFLEKPFEDD RLTAMIESAI
     RQAEPAAKSE AVAQDIAARV ASLSPRERQV MEGLIAGLSN KLIAREYDIS PRTIEVYRAN
     VMTKMQANSL SELVRLAMRA GMLND