ID   FIXK_BRADU              Reviewed;         237 AA.
AC   P29286;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Nitrogen fixation regulation protein FixK;
GN   Name=fixK; OrderedLocusNames=bll6061;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=1551834; DOI=10.1128/jb.174.7.2111-2120.1992;
RA   Anthamatten D., Scherb B., Hennecke H.;
RT   "Characterization of a fixLJ-regulated Bradyrhizobium japonicum gene
RT   sharing similarity with the Escherichia coli fnr and Rhizobium meliloti
RT   fixK genes.";
RL   J. Bacteriol. 174:2111-2120(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: B.japonicum has two FixLJ-dependent FixK homologs that are
CC       activators of the transcription of a group of genes involved in
CC       anaerobic processes such as denitrification and possibly nitrogen
CC       fixation. FixK may bind DNA at the FNR consensus binding site.
CC   -!- INDUCTION: Its expression is maximal under reduced oxygen conditions
CC       and depends on the two-component regulatory system FixLJ.
CC   -!- MISCELLANEOUS: Possesses 4 cysteines which may bind a metal ion
CC       (possibly iron).
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DR   EMBL; M86805; AAA26209.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC51326.1; -; Genomic_DNA.
DR   PIR; B42371; B42371.
DR   RefSeq; NP_772701.1; NC_004463.1.
DR   RefSeq; WP_011088802.1; NZ_CP011360.1.
DR   AlphaFoldDB; P29286; -.
DR   SMR; P29286; -.
DR   STRING; 224911.27354339; -.
DR   EnsemblBacteria; BAC51326; BAC51326; BAC51326.
DR   GeneID; 64025826; -.
DR   KEGG; bja:bll6061; -.
DR   PATRIC; fig|224911.44.peg.6014; -.
DR   eggNOG; COG0664; Bacteria.
DR   HOGENOM; CLU_075053_0_1_5; -.
DR   InParanoid; P29286; -.
DR   OMA; QARDHMV; -.
DR   PhylomeDB; P29286; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd00038; CAP_ED; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR012318; HTH_CRP.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF13545; HTH_Crp_2; 1.
DR   PRINTS; PR00034; HTHCRP.
DR   SMART; SM00100; cNMP; 1.
DR   SMART; SM00419; HTH_CRP; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   PROSITE; PS00042; HTH_CRP_1; 1.
DR   PROSITE; PS51063; HTH_CRP_2; 1.
PE   2: Evidence at transcript level;
KW   Activator; DNA-binding; Nitrogen fixation; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..237
FT                   /note="Nitrogen fixation regulation protein FixK"
FT                   /id="PRO_0000100157"
FT   DOMAIN          156..229
FT                   /note="HTH crp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT   DNA_BIND        189..208
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
SQ   SEQUENCE   237 AA;  26168 MW;  90955FEA2CA6D523 CRC64;
     MKPSVVMIEP NGHFCSDCAI RTSAVCSSLD AAELREFEHL GRRVHFSSGE TVFSEEDITT
     SFYNVLEGVM RLYKLLPDGR RQIVGFALPG DFLGMNLSGR HNFSADAIGA VTVCQFAKAP
     FGRFIEERPQ LLRRINELAI RELSQARDHM VLLGRRSADE KVAAFLLGWR ERLLALKGAS
     DTVPLPMSRQ DIADYLGLTI ETVSRTFTKL ERHGAIAIIH GGISLLDPAR VEALAAA