ID   FIXR_BRADU              Reviewed;         278 AA.
AC   P05406;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 2.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Protein FixR;
GN   Name=fixR; OrderedLocusNames=blr2036;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3313281; DOI=10.1093/nar/15.20.8479;
RA   Thoeny B., Fischer H.-M., Anthamatten D., Bruderer T., Hennecke H.;
RT   "The symbiotic nitrogen fixation regulatory operon (fixRnifA) of
RT   Bradyrhizobium japonicum is expressed aerobically and is subject to a
RT   novel, nifA-independent type of activation.";
RL   Nucleic Acids Res. 15:8479-8499(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=11157954; DOI=10.1128/jb.183.4.1405-1412.2001;
RA   Goettfert M., Roethlisberger S., Kuendig C., Beck C., Marty R.,
RA   Hennecke H.;
RT   "Potential symbiosis-specific genes uncovered by sequencing a 410-kb DNA
RT   region of the Bradyrhizobium japonicum chromosome.";
RL   J. Bacteriol. 183:1405-1412(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; X06167; CAA29530.1; -; Genomic_DNA.
DR   EMBL; AF322013; AAG61007.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC47301.1; -; Genomic_DNA.
DR   PIR; S01065; S01065.
DR   RefSeq; NP_768676.1; NC_004463.1.
DR   RefSeq; WP_011084833.1; NZ_CP011360.1.
DR   AlphaFoldDB; P05406; -.
DR   SMR; P05406; -.
DR   STRING; 224911.27350290; -.
DR   EnsemblBacteria; BAC47301; BAC47301; BAC47301.
DR   GeneID; 64067266; -.
DR   KEGG; bja:blr2036; -.
DR   PATRIC; fig|224911.44.peg.1538; -.
DR   eggNOG; COG1028; Bacteria.
DR   HOGENOM; CLU_010194_1_3_5; -.
DR   InParanoid; P05406; -.
DR   OMA; TIPMRRI; -.
DR   PhylomeDB; P05406; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Nitrogen fixation; Oxidoreductase; Reference proteome.
FT   CHAIN           1..278
FT                   /note="Protein FixR"
FT                   /id="PRO_0000054697"
FT   ACT_SITE        189
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         40..64
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         175
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   278 AA;  29715 MW;  69FDC702072A20A0 CRC64;
     MGLDLPNDNL IRGPLPEAHL DRLVDAVNAR VDRGEPKVML LTGASRGIGH ATAKLFSEAG
     WRIISCARQP FDGERCPWEA GNDDHFQVDL GDHRMLPRAI TEVKKRLAGA PLHALVNNAG
     VSPKTPTGDR MTSLTTSTDT WMRVFHLNLV APILLAQGLF DELRAASGSI VNVTSIAGSR
     VHPFAGSAYA TSKAALASLT RELAHDYAPH GIRVNAIAPG EIRTDMLSPD AEARVVASIP
     LRRVGTPDEV AKVIFFLCSD AASYVTGAEV PINGGQHL