ID   FIXT_RHIME              Reviewed;         115 AA.
AC   O86464;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Transcriptional regulator protein FixT;
DE   AltName: Full=Antikinase FixT;
GN   Name=fixT; OrderedLocusNames=RA0668; ORFNames=SMa1226;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymA (megaplasmid 1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RCR2011 / SU47;
RA   Kahn D.;
RL   Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481432; DOI=10.1073/pnas.161294798;
RA   Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F.,
RA   Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L.,
RA   Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C.,
RA   Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A.,
RA   Long S.R.;
RT   "Nucleotide sequence and predicted functions of the entire Sinorhizobium
RT   meliloti pSymA megaplasmid.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
RN   [4]
RP   IDENTIFICATION, AND CHARACTERIZATION.
RX   PubMed=11902723; DOI=10.1046/j.1365-2958.1997.4501814.x;
RA   Foussard M., Garnerone A.-M., Ni F., Soupene E., Boistard P., Batut J.;
RT   "Negative autoregulation of the Rhizobium meliloti fixK gene is indirect
RT   and requires a newly identified regulator, FixT.";
RL   Mol. Microbiol. 25:27-37(1997).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=10542296; DOI=10.1074/jbc.274.45.32500;
RA   Garnerone A.-M., Cabanes D., Foussard M., Boistard P., Batut J.;
RT   "Inhibition of the FixL sensor kinase by the FixT protein in Sinorhizobium
RT   meliloti.";
RL   J. Biol. Chem. 274:32500-32506(1999).
CC   -!- FUNCTION: Prevents transcription of the intermediate key regulatory
CC       genes nifA and fixK by counteracting the activity of the FixLJ two-
CC       component system. Acts as an inhibitor of the sensor hemoprotein kinase
CC       fixL, preventing the production or the accumulation of its
CC       phosphorylated form.
CC   -!- SUBUNIT: Interacts directly with FixL.
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DR   EMBL; Z21854; CAA79910.1; -; Genomic_DNA.
DR   EMBL; AE006469; AAK65326.1; -; Genomic_DNA.
DR   PIR; D95345; D95345.
DR   RefSeq; NP_435914.1; NC_003037.1.
DR   RefSeq; WP_010967647.1; NC_003037.1.
DR   AlphaFoldDB; O86464; -.
DR   SMR; O86464; -.
DR   EnsemblBacteria; AAK65326; AAK65326; SMa1226.
DR   GeneID; 61599436; -.
DR   KEGG; sme:SMa1226; -.
DR   PATRIC; fig|266834.11.peg.688; -.
DR   HOGENOM; CLU_148627_0_0_5; -.
DR   OMA; MAFALEV; -.
DR   Proteomes; UP000001976; Plasmid pSymA.
PE   1: Evidence at protein level;
KW   Plasmid; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..115
FT                   /note="Transcriptional regulator protein FixT"
FT                   /id="PRO_0000087262"
SQ   SEQUENCE   115 AA;  12218 MW;  C3AAA84458FE0736 CRC64;
     MLDGKTIIVV AADQGLRRSV AFALEVEGYY TESYDSVQKS EASCREALCA IVDDDILRTE
     PQAAAQFLSN RGGRAILLVD GLSALQPPVD YATLTKPFTG ADLLGVINSL VVAAK