FJX1_MOUSE
ID FJX1_MOUSE Reviewed; 450 AA.
AC Q8BQB4; Q9Z1M1;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Four-jointed box protein 1;
DE Short=Four-jointed protein x1;
DE AltName: Full=Four-jointed protein homolog;
DE Flags: Precursor;
GN Name=Fjx1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=129/SvJ;
RX PubMed=10072791; DOI=10.1016/s0925-4773(98)00218-4;
RA Ashery-Padan R., Alvarez-Bolado G., Klamt B., Gessler M., Gruss P.;
RT "Fjx1, the murine homologue of the Drosophila four-jointed gene, codes for
RT a putative secreted protein expressed in restricted domains of the
RT developing and adult brain.";
RL Mech. Dev. 80:213-217(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INDUCTION, PROTEOLYTIC CLEAVAGE, GLYCOSYLATION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=16145673; DOI=10.1002/dvdy.20553;
RA Rock R., Heinrich A.C., Schumacher N., Gessler M.;
RT "Fjx1: a notch-inducible secreted ligand with specific binding sites in
RT developing mouse embryos and adult brain.";
RL Dev. Dyn. 234:602-612(2005).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16059920; DOI=10.1002/dvdy.20515;
RA Rock R., Schrauth S., Gessler M.;
RT "Expression of mouse dchs1, fjx1, and fat-j suggests conservation of the
RT planar cell polarity pathway identified in Drosophila.";
RL Dev. Dyn. 234:747-755(2005).
RN [7]
RP FUNCTION.
RX PubMed=18028897; DOI=10.1016/j.ydbio.2007.09.054;
RA Probst B., Rock R., Gessler M., Vortkamp A., Pueschel A.W.;
RT "The rodent four-jointed ortholog Fjx1 regulates dendrite extension.";
RL Dev. Biol. 312:461-470(2007).
CC -!- FUNCTION: Acts as an inhibitor of dendrite extension and branching.
CC {ECO:0000269|PubMed:18028897}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16145673}.
CC -!- TISSUE SPECIFICITY: Expressed in brain, kidney and lung. In the
CC telencephalon, expressed in the piriform cortex, hippocampus and
CC olfactory bulb. In the diencephalon, expressed in the dorsal thalamus.
CC Expressed in Purkinje cells of the cerebellum and in numerous medullary
CC nuclei. {ECO:0000269|PubMed:10072791, ECO:0000269|PubMed:16059920,
CC ECO:0000269|PubMed:16145673}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryo at 8.5 dpc onward. In the
CC neural plate, expressed in the presumptive forebrain and midbrain and
CC in rhombomere at 4 and 8.5 dpc. Expressed in the limb buds and in the
CC ectoderm of the first branchial arches at 9.5 dpc. In the brain,
CC expressed in the dorsal mesencephalon (tectum) and prosencephalon
CC (presumptive isocortex) at 9.5, 10.5 and 11.5 dpc. In the cortex,
CC expressed in dorsolateral patch of the neuroepithelium at 10.5 dpc.
CC Expressed in the ectoderm of the branchial arch and the oral ectoderm
CC at 10.5 dpc. In the limbs, expressed in the apical ectodermal ridge at
CC 11.5 dpc. Expressed in the telecephalon, ventricles, diencephalon and
CC medulla oblongata at 12.5 dpc. Expressed in the neural tube, cochlear
CC ganglion and olfactory bulb at 14.5 dpc. In the kidney, lung and
CC intestine, expressed in epithelial cells at 14.5 dpc.
CC {ECO:0000269|PubMed:10072791, ECO:0000269|PubMed:16059920,
CC ECO:0000269|PubMed:16145673}.
CC -!- INDUCTION: Up-regulated by Notch. {ECO:0000269|PubMed:16145673}.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:16145673}.
CC -!- PTM: Undergoes proteolytic cleavage. {ECO:0000269|PubMed:16145673}.
CC -!- MISCELLANEOUS: Knockout mice for this gene exhibited an increase in
CC dendrite extension and branching of pyramidal neurons in the CA1 region
CC of the hippocampus.
CC -!- SIMILARITY: Belongs to the FJX1/FJ family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA08764.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AJ009634; CAA08764.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AK051097; BAC34524.1; -; mRNA.
DR EMBL; AL691444; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC051990; AAH51990.1; -; mRNA.
DR CCDS; CCDS16467.1; -.
DR RefSeq; NP_034348.2; NM_010218.2.
DR AlphaFoldDB; Q8BQB4; -.
DR SMR; Q8BQB4; -.
DR STRING; 10090.ENSMUSP00000097270; -.
DR GlyGen; Q8BQB4; 2 sites.
DR PhosphoSitePlus; Q8BQB4; -.
DR MaxQB; Q8BQB4; -.
DR PaxDb; Q8BQB4; -.
DR PRIDE; Q8BQB4; -.
DR ProteomicsDB; 266851; -.
DR Antibodypedia; 25981; 87 antibodies from 23 providers.
DR DNASU; 14221; -.
DR Ensembl; ENSMUST00000099678; ENSMUSP00000097270; ENSMUSG00000075012.
DR GeneID; 14221; -.
DR KEGG; mmu:14221; -.
DR UCSC; uc008lhw.1; mouse.
DR CTD; 24147; -.
DR MGI; MGI:1341907; Fjx1.
DR VEuPathDB; HostDB:ENSMUSG00000075012; -.
DR eggNOG; ENOG502QUJ4; Eukaryota.
DR GeneTree; ENSGT00390000016768; -.
DR HOGENOM; CLU_033850_0_0_1; -.
DR InParanoid; Q8BQB4; -.
DR OMA; YRRHEPR; -.
DR OrthoDB; 1414662at2759; -.
DR PhylomeDB; Q8BQB4; -.
DR TreeFam; TF324767; -.
DR BioGRID-ORCS; 14221; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q8BQB4; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8BQB4; protein.
DR Bgee; ENSMUSG00000075012; Expressed in subiculum and 191 other tissues.
DR Genevisible; Q8BQB4; MM.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0007267; P:cell-cell signaling; IBA:GO_Central.
DR GO; GO:0010842; P:retina layer formation; IGI:MGI.
DR InterPro; IPR024868; FJX1/FJ.
DR PANTHER; PTHR13147; PTHR13147; 1.
DR PRINTS; PR02072; 4JOINTEDBOX1.
PE 1: Evidence at protein level;
KW Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..450
FT /note="Four-jointed box protein 1"
FT /id="PRO_0000333046"
FT REGION 36..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 88..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..111
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 248
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 277
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 109
FT /note="L -> C (in Ref. 1; CAA08764)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 450 AA; 50396 MW; 4DC00C72457ECE87 CRC64;
MGRKMRGAAA AAGLWLLALS SLLTLWGGLL PPRTELPASR PPEDRLPPHP IQSGGPAPEP
RFPLPPPLVW DARGGSLKTF RALLTLAAGA DNPPRRHQDD RGRHEPSGLS WPEERRAVHG
GVFWSRGLEE QVPRGFSEAQ AAAWLEVARG ARVVALDRGG CGRSSNRLAR FADGTRACVR
YGINPEQIQG EALSYYLARL LGLQRHVPPL ALARVEARGA QWVQVQEELR TAHWTEGSVV
SLTRWLPNLT DVVVPEPWRS EDGRLRPLRD AGGELTNLSQ AELVDLVQWT DLILFDYLTA
NFDRLVSNLF SLQWDPRVMH RATSNLHRGP GGALVFLDNE AGLVHGYRVA GMWDKYNEPL
LQSVCVFRER TARRVLELHR GQDAAARLLR LYSRHEPRFP ELAELSEPHA QLLQRRLDFL
AKHILHCKAK YGRRPGDLIT LRGREGLGYE
