AKR1_ASHGO
ID AKR1_ASHGO Reviewed; 724 AA.
AC Q755Y0;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Palmitoyltransferase AKR1;
DE EC=2.3.1.225;
DE AltName: Full=Ankyrin repeat-containing protein AKR1;
GN Name=AKR1; OrderedLocusNames=AER388C;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Palmitoyltransferase specific for casein kinase 1.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225;
CC -!- SUBCELLULAR LOCATION: Early endosome membrane; Multi-pass membrane
CC protein. Golgi apparatus membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC AKR/ZDHHC17 subfamily. {ECO:0000305}.
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DR EMBL; AE016818; AAS53067.1; -; Genomic_DNA.
DR RefSeq; NP_985243.1; NM_210597.1.
DR AlphaFoldDB; Q755Y0; -.
DR SMR; Q755Y0; -.
DR STRING; 33169.AAS53067; -.
DR EnsemblFungi; AAS53067; AAS53067; AGOS_AER388C.
DR GeneID; 4621459; -.
DR KEGG; ago:AGOS_AER388C; -.
DR eggNOG; KOG0509; Eukaryota.
DR HOGENOM; CLU_012510_1_1_1; -.
DR InParanoid; Q755Y0; -.
DR OMA; PWMAGIF; -.
DR Proteomes; UP000000591; Chromosome V.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IEA:EnsemblFungi.
DR GO; GO:0016409; F:palmitoyltransferase activity; IBA:GO_Central.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0090029; P:negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion; IEA:EnsemblFungi.
DR GO; GO:0018345; P:protein palmitoylation; IBA:GO_Central.
DR GO; GO:0006612; P:protein targeting to membrane; IEA:EnsemblFungi.
DR GO; GO:0030100; P:regulation of endocytosis; IEA:EnsemblFungi.
DR Gene3D; 1.25.40.20; -; 3.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF01529; DHHC; 1.
DR SMART; SM00248; ANK; 5.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 4.
DR PROSITE; PS50216; DHHC; 1.
PE 3: Inferred from homology;
KW Acyltransferase; ANK repeat; Endosome; Golgi apparatus; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Repeat; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..724
FT /note="Palmitoyltransferase AKR1"
FT /id="PRO_0000212916"
FT TOPO_DOM 1..308
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..331
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..360
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..392
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..489
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 511..543
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 544..564
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 565..724
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 54..84
FT /note="ANK 1"
FT REPEAT 90..119
FT /note="ANK 2"
FT REPEAT 124..153
FT /note="ANK 3"
FT REPEAT 157..187
FT /note="ANK 4"
FT REPEAT 191..220
FT /note="ANK 5"
FT REPEAT 224..253
FT /note="ANK 6"
FT DOMAIN 446..496
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT ACT_SITE 476
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 724 AA; 81922 MW; C5C5BBB4D272A99C CRC64;
MSGQLNVAED SVLLDDISIS SIQPIVSDVP AAEDVGDEDH ISGASIAENR PKDPTLVKYH
AACQRGDLKT VKDMIEAGVI DVKADWDEEE QVSGLHWASA NNRLNLVRYL IAQGADVNIK
GGDLEATPLH WASKSGYVYI VHCLLEHGAD PLITDRQGYN LLHTSTFSSE VMLITYVLFT
GQIPVDSPDP TGKTALHWAA YQGDPNTVEA LLKFDADVRV VDTGGFTPLH WATVKGHPHV
LKALIEHGSD VFLKNNDGKN ALMIAQEMNT QKALQNALYE CGFNKDGFAI RKYFKNPMHA
KMVTFFTPWL ILGLILSFFA YFSILLAILG CLLTVLAAGY GLYNFVFPSF ILMKRIVIFK
TPLLAGILSG TIFWLLYVWL FKMLPATFDD EPILNLTVFA LFAGVVFLLT KLLQSDPGYI
VPATDHNQIR ATIIELLRVG KYDSKHFCIH SWIRLPLRAK YKRFVHSVIL RYDHYCPWIY
NYIGFRNHKI FIYFILLLDL GILALAKLCL EYFDELKDHA KNKDALKCSI LSKDLCAGFT
YDPFTLFLLE WVCVQGMWIL ALSFVQFFEC LKGVTEHEFA HWQRRNRGVV GREHVFNTAP
EELMEEDPEE ERNKLRSLGA AGQRHCCSTL TTATGFDRFL SVIGSSLGRQ PRTTHNPINY
PIETDYGWKQ NLKDFWLTSD ISAPMWRRIL LPPVGTRGLL NGQEVDYQKL YNLPERVISI
EEIV
