FKH02_CAEEL
ID FKH02_CAEEL Reviewed; 270 AA.
AC Q22510; Q95QA9;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Forkhead box protein fkh-2 {ECO:0000305};
DE AltName: Full=Forkhead transcription factor family member fkh-2 {ECO:0000303|PubMed:11044397, ECO:0000312|WormBase:T14G12.4a};
GN Name=fkh-2 {ECO:0000312|WormBase:T14G12.4a};
GN ORFNames=T14G12.4 {ECO:0000303|PubMed:11044397,
GN ECO:0000312|WormBase:T14G12.4a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=11044397; DOI=10.1242/dev.127.22.4825;
RA Molin L., Mounsey A., Aslam S., Bauer P., Young J., James M.,
RA Sharma-Oates A., Hope I.A.;
RT "Evolutionary conservation of redundancy between a diverged pair of
RT forkhead transcription factor homologues.";
RL Development 127:4825-4835(2000).
CC -!- FUNCTION: Transcription factor (By similarity). Plays a role in
CC embryogenesis and later development, perhaps acting redundantly with
CC forkhead protein pes-1 (PubMed:11044397).
CC {ECO:0000250|UniProtKB:Q12952, ECO:0000269|PubMed:11044397}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089,
CC ECO:0000269|PubMed:11044397}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:T14G12.4a};
CC IsoId=Q22510-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:T14G12.4b};
CC IsoId=Q22510-2; Sequence=VSP_061479;
CC -!- DEVELOPMENTAL STAGE: Expressed in the 4 descendants of the D blastomere
CC in embryos at about the 185 cells stage and in many other unidentified
CC cells located more anteriorly. {ECO:0000269|PubMed:11044397}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown by injection into adults
CC causes slow and spatially restricted movement of about 30% of their L1
CC larval progeny, which nonetheless grow normally and do not display any
CC obvious phenotype as late larvae or adults (PubMed:11044397). However,
CC simultaneous knockdown of forkhead gene pes-1 causes 12% of eggs
CC produced by hermaphrodites to arrest development at late stages of
CC embryogenesis and 81% arrest after hatching as L1 stage larvae
CC (PubMed:11044397). {ECO:0000269|PubMed:11044397}.
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DR EMBL; BX284606; CCD62370.1; -; Genomic_DNA.
DR EMBL; BX284606; CCD62371.1; -; Genomic_DNA.
DR PIR; T16880; T16880.
DR RefSeq; NP_508644.1; NM_076243.6.
DR RefSeq; NP_508645.1; NM_076244.3.
DR AlphaFoldDB; Q22510; -.
DR SMR; Q22510; -.
DR DIP; DIP-25543N; -.
DR IntAct; Q22510; 5.
DR STRING; 6239.T14G12.4a; -.
DR PaxDb; Q22510; -.
DR EnsemblMetazoa; T14G12.4a.1; T14G12.4a.1; WBGene00001434. [Q22510-1]
DR EnsemblMetazoa; T14G12.4b.1; T14G12.4b.1; WBGene00001434. [Q22510-2]
DR GeneID; 180663; -.
DR KEGG; cel:CELE_T14G12.4; -.
DR UCSC; T14G12.4a; c. elegans. [Q22510-1]
DR CTD; 180663; -.
DR WormBase; T14G12.4a; CE04965; WBGene00001434; fkh-2.
DR WormBase; T14G12.4b; CE29342; WBGene00001434; fkh-2.
DR eggNOG; KOG2294; Eukaryota.
DR GeneTree; ENSGT00940000165356; -.
DR HOGENOM; CLU_1070538_0_0_1; -.
DR InParanoid; Q22510; -.
DR OMA; KDSKSPC; -.
DR OrthoDB; 1270467at2759; -.
DR PhylomeDB; Q22510; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001434; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR ExpressionAtlas; Q22510; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0016358; P:dendrite development; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IGI:WormBase.
DR GO; GO:0002119; P:nematode larval development; IGI:WormBase.
DR GO; GO:0048665; P:neuron fate specification; IMP:WormBase.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR018122; TF_fork_head_CS_1.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00657; FORK_HEAD_1; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..270
FT /note="Forkhead box protein fkh-2"
FT /id="PRO_0000455283"
FT DNA_BIND 93..188
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 32..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..71
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..94
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..102
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_061479"
SQ SEQUENCE 270 AA; 30491 MW; 7C49116E5EC76175 CRC64;
MARFSILDLC PDLVEEAVKI EQLHISTQPC IKQESTDISD PSTSIDTTDT MSTDYLHDES
IDDERSESTL SKDSKSPSSS NSEEKTPSSP NDKPPFSYNA LIMMAIKDSP EKRLTLAGIY
EYIVTNYPFY RDNKQGWQNS IRHNLSLNKC FVKVPRNFDD PGKGNYWMLD ATAEDEVFIG
GATGKLRRRP SSLSRARMDA YKQYGAAAAN LFPYFSPGMP ALPRHPYLTA PNGFLPRQMM
PIPAMPQVFA QPELLQLYIN QQQSLFTKLQ
