FKH6_CAEEL
ID FKH6_CAEEL Reviewed; 323 AA.
AC Q10924;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Forkhead transcription factor fkh-6 {ECO:0000312|WormBase:B0286.5};
GN Name=fkh-6 {ECO:0000312|WormBase:B0286.5};
GN ORFNames=B0286.5 {ECO:0000312|WormBase:B0286.5};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF MET-1.
RX PubMed=14993191; DOI=10.1242/dev.01012;
RA Chang W., Tilmann C., Thoemke K., Markussen F.H., Mathies L.D., Kimble J.,
RA Zarkower D.;
RT "A forkhead protein controls sexual identity of the C. elegans male somatic
RT gonad.";
RL Development 131:1425-1436(2004).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=20553900; DOI=10.1016/j.ydbio.2010.05.516;
RA Kalis A.K., Murphy M.W., Zarkower D.;
RT "EGL-5/ABD-B plays an instructive role in male cell fate determination in
RT the C. elegans somatic gonad.";
RL Dev. Biol. 344:827-835(2010).
CC -!- FUNCTION: Probable transcription factor (PubMed:14993191,
CC PubMed:20553900). Binds to the DNA sequence motif 5'-
CC [TA]TGTT[TG]T[TG][ATG]TT-3' (PubMed:20553900). Regulates sexual
CC dimorphism in the gonad, promoting male gonadal cell fates in
CC chromosomally (XO) male animals, yet plays a role in gonadogenesis in
CC both sexes; probably acts downstream of terminal regulator of sex
CC determination tra-1, to control early gonadogenesis (PubMed:14993191).
CC Positively modulates expression of homeobox protein egl-5, probably
CC acting indirectly, during early gonadal development (PubMed:20553900).
CC {ECO:0000269|PubMed:14993191, ECO:0000269|PubMed:20553900}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the somatic gonadal precursor cells,
CC Z1 and Z4, of XO and XX L1 stage larvae (PubMed:14993191). Expression
CC is sexually dimorphic later in larval L1 stage and appears to be gonad-
CC specific (PubMed:14993191). {ECO:0000269|PubMed:14993191}.
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DR EMBL; BX284602; CCD61668.1; -; Genomic_DNA.
DR PIR; T15311; T15311.
DR RefSeq; NP_494775.1; NM_062374.1.
DR AlphaFoldDB; Q10924; -.
DR SMR; Q10924; -.
DR IntAct; Q10924; 54.
DR STRING; 6239.B0286.5; -.
DR PaxDb; Q10924; -.
DR EnsemblMetazoa; B0286.5.1; B0286.5.1; WBGene00001438.
DR GeneID; 181907; -.
DR KEGG; cel:CELE_B0286.5; -.
DR UCSC; B0286.5; c. elegans.
DR CTD; 181907; -.
DR WormBase; B0286.5; CE03865; WBGene00001438; fkh-6.
DR eggNOG; KOG2294; Eukaryota.
DR GeneTree; ENSGT00940000173882; -.
DR HOGENOM; CLU_850554_0_0_1; -.
DR InParanoid; Q10924; -.
DR OMA; GQSCAND; -.
DR OrthoDB; 1154939at2759; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001438; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IC:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0008406; P:gonad development; IGI:UniProtKB.
DR GO; GO:0019100; P:male germ-line sex determination; IGI:WormBase.
DR GO; GO:0008584; P:male gonad development; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR018122; TF_fork_head_CS_1.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00657; FORK_HEAD_1; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..323
FT /note="Forkhead transcription factor fkh-6"
FT /id="PRO_0000454565"
FT DNA_BIND 21..122
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT MUTAGEN 1
FT /note="M->I: In ez16; defects in gonad morphology; failure
FT to elongate normally and feminization of male (X0) gonad,
FT such as developing a vulva, are the most drastic and most
FT frequent abnormalities. Hermaphrodites are infertile.
FT Abolishes expression of homeobox egl-5 in somatic gonadal
FT precursor cells, Z1 and Z4, during larval L1 stage."
FT /evidence="ECO:0000269|PubMed:14993191,
FT ECO:0000269|PubMed:20553900"
SQ SEQUENCE 323 AA; 36421 MW; 00A2BA04326ACA2A CRC64;
MTRHQTHLPF HIVQEGNSID KPPYSYVALI AMAIDASPDK RMTLNQIYKF IEAKFPYYRD
ADAKRKQGWQ NSIRHNLSLN DCFVKKARDG QSCANDRKGN YWQMVADNAP QFDNGNFKRR
RVKRLGIGKM GYANTTETTE TGTILQQQLP FFNGLKWPQN IQTMDPFQFF KFQYPNSITD
SANTSQINNS SSSSSSFDTS IYTSAFPIPT SHFDTNLVAP SQPPPVVSDV EVVPSDTVKE
EVLVDMKPLI PGISSTTFLT SLQMTDPRSI EQQQLLSTAS NMYPNAFMPP YTNWSCQPTT
TFTGLSFDDP SLYGYGQQFP ASS
