FKH9_CAEEL
ID FKH9_CAEEL Reviewed; 300 AA.
AC Q21187; Q2L6X6; Q7Z136;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Forkhead transcription factor fkh-9 {ECO:0000312|WormBase:K03C7.2a};
GN Name=fkh-9 {ECO:0000312|WormBase:K03C7.2a};
GN ORFNames=K03C7.2 {ECO:0000312|WormBase:K03C7.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION.
RX PubMed=26963674; DOI=10.1016/j.ydbio.2016.02.031;
RA Goszczynski B., Captan V.V., Danielson A.M., Lancaster B.R., McGhee J.D.;
RT "A 44 bp intestine-specific hermaphrodite-specific enhancer from the C.
RT elegans vit-2 vitellogenin gene is directly regulated by ELT-2, MAB-3, FKH-
RT 9 and DAF-16 and indirectly regulated by the germline, by daf-2/insulin
RT signaling and by the TGF-beta/Sma/Mab pathway.";
RL Dev. Biol. 413:112-127(2016).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=26675724; DOI=10.1038/nature16483;
RA Kaletsky R., Lakhina V., Arey R., Williams A., Landis J., Ashraf J.,
RA Murphy C.T.;
RT "The C. elegans adult neuronal IIS/FOXO transcriptome reveals adult
RT phenotype regulators.";
RL Nature 529:92-96(2016).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP GLY-111.
RX PubMed=30287474; DOI=10.1534/genetics.118.301450;
RA Tillman E.J., Richardson C.E., Cattie D.J., Reddy K.C., Lehrbach N.J.,
RA Droste R., Ruvkun G., Kim D.H.;
RT "Endoplasmic Reticulum Homeostasis Is Modulated by the Forkhead
RT Transcription Factor FKH-9 During Infection of Caenorhabditis elegans.";
RL Genetics 210:1329-1337(2018).
CC -!- FUNCTION: Transcription factor (PubMed:26963674). Binds to the
CC regulatory elements of genes that contain the sequence motif 5'-
CC TTGTTTCT-3' (PubMed:26963674). Involved in regulating intestinal
CC transcription of vitellogenin vit-2, acting in concert with
CC transcription factors elt-2, mab-3 and daf-16, and also the TGF-
CC beta/Sma/Mab pathway (PubMed:26963674). Functions downstream of the
CC insulin/IGF-1-like signaling (IIS) mediated pathway, in regeneration of
CC axons after injury and in short-term memory, perhaps acting in neurons,
CC and in modulation of longevity, perhaps acting non-neuronally
CC (PubMed:26675724). Plays a role in the modulation of endoplasmic
CC reticulum (ER) homeostasis during chemical and pathogen stress,
CC including exposure to the Gram-negative bacterium P.aeruginosa
CC (PubMed:30287474). {ECO:0000269|PubMed:26675724,
CC ECO:0000269|PubMed:26963674, ECO:0000269|PubMed:30287474}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089,
CC ECO:0000269|PubMed:26675724, ECO:0000269|PubMed:30287474}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a {ECO:0000312|WormBase:K03C7.2a};
CC IsoId=Q21187-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:K03C7.2b};
CC IsoId=Q21187-2; Sequence=VSP_061276;
CC Name=c {ECO:0000312|WormBase:K03C7.2c};
CC IsoId=Q21187-3; Sequence=VSP_061275;
CC -!- TISSUE SPECIFICITY: Expressed in mechanosensory neurons.
CC {ECO:0000269|PubMed:26675724}.
CC -!- DEVELOPMENTAL STAGE: Expressed in neurons and intestinal cells in L2
CC stage larvae. {ECO:0000269|PubMed:30287474}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown severely impairs axon
CC regeneration in a daf-2 mutant background, in a daf-16-dependent-manner
CC (PubMed:26675724). Impairs short-term associative memory in a daf-2
CC mutant background in a daf-16-dependent-manner (PubMed:26675724).
CC Reduces longevity in a daf-2 mutant background (PubMed:26675724).
CC {ECO:0000269|PubMed:26675724}.
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DR EMBL; BX284606; CCD70485.1; -; Genomic_DNA.
DR EMBL; BX284606; CCD70486.1; -; Genomic_DNA.
DR EMBL; BX284606; CCD70487.1; -; Genomic_DNA.
DR PIR; T16544; T16544.
DR RefSeq; NP_001024760.1; NM_001029589.3.
DR AlphaFoldDB; Q21187; -.
DR SMR; Q21187; -.
DR STRING; 6239.K03C7.2a; -.
DR PaxDb; Q21187; -.
DR PeptideAtlas; Q21187; -.
DR EnsemblMetazoa; K03C7.2a.1; K03C7.2a.1; WBGene00001441. [Q21187-1]
DR EnsemblMetazoa; K03C7.2b.1; K03C7.2b.1; WBGene00001441. [Q21187-2]
DR EnsemblMetazoa; K03C7.2c.1; K03C7.2c.1; WBGene00001441. [Q21187-3]
DR EnsemblMetazoa; K03C7.2c.2; K03C7.2c.2; WBGene00001441. [Q21187-3]
DR EnsemblMetazoa; K03C7.2c.3; K03C7.2c.3; WBGene00001441. [Q21187-3]
DR EnsemblMetazoa; K03C7.2c.4; K03C7.2c.4; WBGene00001441. [Q21187-3]
DR GeneID; 180670; -.
DR KEGG; cel:CELE_K03C7.2; -.
DR UCSC; K03C7.2a; c. elegans. [Q21187-1]
DR CTD; 180670; -.
DR WormBase; K03C7.2a; CE31196; WBGene00001441; fkh-9.
DR WormBase; K03C7.2b; CE34332; WBGene00001441; fkh-9.
DR WormBase; K03C7.2c; CE39581; WBGene00001441; fkh-9.
DR eggNOG; KOG2294; Eukaryota.
DR HOGENOM; CLU_998283_0_0_1; -.
DR InParanoid; Q21187; -.
DR OMA; LASTIQM; -.
DR OrthoDB; 1430006at2759; -.
DR Reactome; R-CEL-9018519; Estrogen-dependent gene expression.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001441; Expressed in larva and 3 other tissues.
DR ExpressionAtlas; Q21187; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IMP:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0031103; P:axon regeneration; IGI:WormBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0098542; P:defense response to other organism; IGI:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; IGI:WormBase.
DR GO; GO:0036503; P:ERAD pathway; IMP:UniProtKB.
DR GO; GO:0007612; P:learning; IGI:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0010498; P:proteasomal protein catabolic process; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IGI:UniProtKB.
DR GO; GO:0007614; P:short-term memory; IGI:WormBase.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..300
FT /note="Forkhead transcription factor fkh-9"
FT /id="PRO_0000454295"
FT DNA_BIND 66..161
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 153..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..179
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 180..194
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..206
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_061275"
FT VAR_SEQ 1..20
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_061276"
FT MUTAGEN 111
FT /note="G->E: In qd197; suppresses larval lethality and
FT defects in the endoplasmic reticulum (ER) in an xbp-1
FT mutant background, when exposed to the Gram-negative
FT bacterium P.aeruginosa. Enhances resistance to tunicamycin,
FT probably as a result of enhanced ER stress resistance.
FT Enhances sensitivity to proteasome inhibition."
FT /evidence="ECO:0000269|PubMed:30287474"
SQ SEQUENCE 300 AA; 33345 MW; EF24FBC0FB4D8FA0 CRC64;
MCSTMTATMN QTICEKQNIT MDLLRSPIQI PSASDTIKIE TPSTISSQPS PIQTPLAAAS
SDNFERPSLS YKDLIIEAID RSPEKRLKLN EIYQVIRLLH PYYRHRPDQW GWQNSIRHNL
SLHDCFVKLP LKQTSASGVV GHFWTVVPEL SDKQTLRRRN RQQPRALAKK SDAGRTLSRD
DRGSSGSGET SPSPSQPSIS PPNENPMPSV QALNVLELLS GMNDYKGTLF QNNYRTNLIQ
DNSAVNGLQN LLTTTLMQIN PQLGALLSLA NLNNLTTNQL QIASPSLSPI KAESQSFLLQ
