FKH_DROME
ID FKH_DROME Reviewed; 510 AA.
AC P14734; Q9VAV0;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 194.
DE RecName: Full=Protein fork head;
GN Name=fkh; ORFNames=CG10002;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2566386; DOI=10.1016/0092-8674(89)90133-5;
RA Weigel D., Juergens G., Kuettner F., Seifert E., Jaeckle H.;
RT "The homeotic gene fork head encodes a nuclear protein and is expressed in
RT the terminal regions of the Drosophila embryo.";
RL Cell 57:645-658(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187; SER-190; SER-320;
RP SER-322 AND SER-330, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Fkh promotes terminal as opposed to segmental development. In
CC the absence of fkh, this developmental switch does not occur. The
CC nuclear localization of the fkh protein suggest that fkh regulates the
CC transcription of other, subordinate, genes.
CC -!- SUBCELLULAR LOCATION: Nucleus.
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DR EMBL; J03177; AAA28535.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56798.1; -; Genomic_DNA.
DR PIR; A32380; A32380.
DR RefSeq; NP_001163762.1; NM_001170291.1.
DR RefSeq; NP_001287574.1; NM_001300645.1.
DR RefSeq; NP_524542.1; NM_079818.3.
DR AlphaFoldDB; P14734; -.
DR SMR; P14734; -.
DR BioGRID; 68259; 24.
DR DIP; DIP-17045N; -.
DR IntAct; P14734; 5.
DR STRING; 7227.FBpp0289487; -.
DR iPTMnet; P14734; -.
DR PaxDb; P14734; -.
DR EnsemblMetazoa; FBtr0085321; FBpp0084690; FBgn0000659.
DR EnsemblMetazoa; FBtr0300259; FBpp0289487; FBgn0000659.
DR EnsemblMetazoa; FBtr0345227; FBpp0311419; FBgn0000659.
DR GeneID; 43383; -.
DR KEGG; dme:Dmel_CG10002; -.
DR CTD; 43383; -.
DR FlyBase; FBgn0000659; fkh.
DR VEuPathDB; VectorBase:FBgn0000659; -.
DR eggNOG; KOG3563; Eukaryota.
DR GeneTree; ENSGT00940000155999; -.
DR HOGENOM; CLU_027910_4_1_1; -.
DR InParanoid; P14734; -.
DR OMA; CMSAVGY; -.
DR OrthoDB; 1181467at2759; -.
DR PhylomeDB; P14734; -.
DR Reactome; R-DME-9018519; Estrogen-dependent gene expression.
DR SignaLink; P14734; -.
DR BioGRID-ORCS; 43383; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 43383; -.
DR PRO; PR:P14734; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000659; Expressed in embryonic hindgut (Drosophila) and 83 other tissues.
DR ExpressionAtlas; P14734; baseline and differential.
DR Genevisible; P14734; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0019904; F:protein domain specific binding; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:FlyBase.
DR GO; GO:0042023; P:DNA endoreduplication; IMP:FlyBase.
DR GO; GO:0035072; P:ecdysone-mediated induction of salivary gland cell autophagic cell death; IDA:FlyBase.
DR GO; GO:0001706; P:endoderm formation; TAS:FlyBase.
DR GO; GO:0008286; P:insulin receptor signaling pathway; IDA:FlyBase.
DR GO; GO:0007443; P:Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:FlyBase.
DR GO; GO:0030308; P:negative regulation of cell growth; IMP:FlyBase.
DR GO; GO:0040015; P:negative regulation of multicellular organism growth; IMP:FlyBase.
DR GO; GO:0043069; P:negative regulation of programmed cell death; IDA:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; TAS:FlyBase.
DR GO; GO:0061101; P:neuroendocrine cell differentiation; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0007431; P:salivary gland development; IMP:FlyBase.
DR GO; GO:0007435; P:salivary gland morphogenesis; IMP:FlyBase.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR013638; Fork-head_N.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR018122; TF_fork_head_CS_1.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR Pfam; PF08430; Forkhead_N; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00657; FORK_HEAD_1; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..510
FT /note="Protein fork head"
FT /id="PRO_0000091896"
FT DNA_BIND 209..300
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 1..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 175..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 309..359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 339..353
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 187
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 190
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 320
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 322
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 330
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 510 AA; 54286 MW; 2B86E807CBF7881B CRC64;
MQKLYAEPPP SSAPVSMASS GGGGPPSGGG GGGGGGGGGG PPPPSNNNPN PTSNGGSMSP
LARSAYTMNS MGLPVGGMSS VSPQAAATFS SSVLDSAAAV ASMSASMSAS MSASMNASMN
GSMGAAAMNS MGGNCMTPSS MSYASMGSPL GNMGGCMAMS AASMSAAGLS GTYGAMPPGS
REMETGSPNS LGRSRVDKPT TYRRSYTHAK PPYSYISLIT MAIQNNPTRM LTLSEIYQFI
MDLFPFYRQN QQRWQNSIRH SLSFNDCFVK IPRTPDKPGK GSFWTLHPDS GNMFENGCYL
RRQKRFKDEK KEAIRQLHKS PSHSSLEATS PGKKDHEDSH HMHHHHHSRL DHHQHHKEAG
GASIAGVNVL SAAHSKDAEA LAMLHANAEL CLSQQPQHVP THHHHQHHQL QQEELSAMMA
NRCHPSLITD YHSSMHPLKQ EPSGYTPSSH PFSINRLLPT ESKADIKMYD MSQYAGYNAL
SPLTNSHAAL GQDSYYQSLG YHAPAGTTSL
