FL1_MAIZE
ID FL1_MAIZE Reviewed; 302 AA.
AC A8DMN5; B6TSW0;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Protein FLOURY 1 {ECO:0000303|PubMed:17693529};
GN Name=FL1 {ECO:0000303|PubMed:17693529};
GN ORFNames=GRMZM2G094532 {ECO:0000305};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP MUTAGENESIS OF SER-27, TISSUE SPECIFICITY, FUNCTION, AND INTERACTION WITH
RP ALPHA-ZEINS.
RX PubMed=17693529; DOI=10.1105/tpc.107.053538;
RA Holding D.R., Otegui M.S., Li B., Meeley R.B., Dam T., Hunter B.G.,
RA Jung R., Larkins B.A.;
RT "The maize floury1 gene encodes a novel endoplasmic reticulum protein
RT involved in zein protein body formation.";
RL Plant Cell 19:2569-2582(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. B73;
RX PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA Walbot V., Yu Y.;
RT "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL PLoS Genet. 5:E1000740-E1000740(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
RN [5]
RP INTERACTION WITH OP10.
RX PubMed=27541862; DOI=10.1371/journal.pgen.1006270;
RA Yao D., Qi W., Li X., Yang Q., Yan S., Ling H., Wang G., Wang G., Song R.;
RT "Maize opaque10 encodes a cereal-specific protein that is essential for the
RT proper distribution of zeins in endosperm protein bodies.";
RL PLoS Genet. 12:E1006270-E1006270(2016).
CC -!- FUNCTION: Involved in protein body development and 22 kDa alpha-zein
CC localization. {ECO:0000269|PubMed:17693529}.
CC -!- SUBUNIT: Interacts (via C-terminus) with both 22 kDa and 19 kDa alpha-
CC zeins (PubMed:17693529). Interacts (via C-terminus) with OP10 (via N-
CC terminus) (PubMed:27541862). {ECO:0000269|PubMed:17693529,
CC ECO:0000269|PubMed:27541862}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:17693529}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Found in the endoplasmic reticulum surrounding the
CC protein body, but not in the cisternal membranes.
CC {ECO:0000269|PubMed:17693529}.
CC -!- TISSUE SPECIFICITY: Expressed in endosperm. Not detected in embryo,
CC leaves and roots. {ECO:0000269|PubMed:17693529}.
CC -!- DEVELOPMENTAL STAGE: Accumulates at a high level in endosperm during
CC the period of zein synthesis and protein body development and declines
CC to a low level at kernel maturity. {ECO:0000269|PubMed:17693529}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EF536720; ABS85200.1; -; mRNA.
DR EMBL; BT040382; ACF85387.1; -; mRNA.
DR EMBL; EU968075; ACG40193.1; -; mRNA.
DR EMBL; EU969723; ACG41841.1; -; mRNA.
DR RefSeq; NP_001106064.1; NM_001112594.1.
DR RefSeq; NP_001144622.1; NM_001151150.1.
DR AlphaFoldDB; A8DMN5; -.
DR SMR; A8DMN5; -.
DR STRING; 4577.GRMZM2G094532_P01; -.
DR PaxDb; A8DMN5; -.
DR PRIDE; A8DMN5; -.
DR EnsemblPlants; Zm00001eb080540_T001; Zm00001eb080540_P001; Zm00001eb080540.
DR GeneID; 100126364; -.
DR Gramene; Zm00001eb080540_T001; Zm00001eb080540_P001; Zm00001eb080540.
DR KEGG; zma:100126364; -.
DR eggNOG; ENOG502S6F3; Eukaryota.
DR HOGENOM; CLU_057945_0_0_1; -.
DR OMA; QWVIMKS; -.
DR OrthoDB; 1399226at2759; -.
DR Proteomes; UP000007305; Chromosome 2.
DR ExpressionAtlas; A8DMN5; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0080115; F:myosin XI tail binding; IEA:UniProt.
DR InterPro; IPR040284; FL1-like.
DR InterPro; IPR007656; GTD-bd.
DR PANTHER; PTHR31422:SF2; PTHR31422:SF2; 1.
DR Pfam; PF04576; Zein-binding; 1.
DR PROSITE; PS51775; GTD_BINDING; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..302
FT /note="Protein FLOURY 1"
FT /id="PRO_0000438206"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 193..299
FT /note="GTD-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01111"
FT REGION 160..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 283..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 199..254
FT /evidence="ECO:0000255"
FT COMPBIAS 174..188
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 27
FT /note="S->G: In fl1-ref; no effect on zeins accumulation,
FT but opaque kernels."
FT /evidence="ECO:0000269|PubMed:17693529"
FT CONFLICT 66
FT /note="T -> A (in Ref. 4; ACG40193/ACG41841)"
FT /evidence="ECO:0000305"
FT CONFLICT 187
FT /note="T -> I (in Ref. 4; ACG40193/ACG41841)"
FT /evidence="ECO:0000305"
FT CONFLICT 280
FT /note="G -> S (in Ref. 4; ACG40193/ACG41841)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 302 AA; 32586 MW; 7567BEFA9FFDF984 CRC64;
MGGNNDGVSG AACLKPLNGV SPTYVPSAGA GALYFLIGSG LGVVAVLHAS GLAEVSGEWA
SAARWTEVSR EWPSAARWAA QLAGSVGAQR LLLATSLLFL AVSVWRLARR CAAVEGRVGS
TDSAVRALHV GGVVCAVCGS KIRALKRGCR GVERTRSVDS SKPVSRSLAA EFDQEADGEE
EDNAGETSDP DDGSVQYLRR RLKEEMLLKE VALEELEKER HAAASAADEA MSKIACLRSE
KALVEREARQ FQEMMQQKQM YDRQVIESLQ WVIMKSGMQG WEPEAITDRA LSETSEDDRD
KK
