FL2D_DANRE
ID FL2D_DANRE Reviewed; 423 AA.
AC Q7SXL7;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Pre-mRNA-splicing regulator WTAP {ECO:0000305};
DE AltName: Full=Female-lethal(2)D homolog {ECO:0000250|UniProtKB:Q15007};
DE AltName: Full=WT1-associated protein {ECO:0000250|UniProtKB:Q15007};
DE AltName: Full=Wilms tumor 1-associating protein {ECO:0000250|UniProtKB:Q15007};
GN Name=wtap {ECO:0000250|UniProtKB:Q15007};
GN ORFNames=ch211-195e3.2 {ECO:0000303|PubMed:23594743},
GN zgc:66202 {ECO:0000303|Ref.2};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=24407421; DOI=10.1038/cr.2014.3;
RA Ping X.L., Sun B.F., Wang L., Xiao W., Yang X., Wang W.J., Adhikari S.,
RA Shi Y., Lv Y., Chen Y.S., Zhao X., Li A., Yang Y., Dahal U., Lou X.M.,
RA Liu X., Huang J., Yuan W.P., Zhu X.F., Cheng T., Zhao Y.L., Wang X.,
RA Danielsen J.M., Liu F., Yang Y.G.;
RT "Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine
RT methyltransferase.";
RL Cell Res. 24:177-189(2014).
CC -!- FUNCTION: Associated component of the WMM complex, a complex that
CC mediates N6-methyladenosine (m6A) methylation of RNAs, a modification
CC that plays a role in the efficiency of mRNA splicing and RNA
CC processing. {ECO:0000250|UniProtKB:Q15007}.
CC -!- SUBUNIT: Component of the WMM complex, a N6-methyltransferase complex
CC composed of a catalytic subcomplex, named MAC, and of an associated
CC subcomplex, named MACOM. Component of the MACOM subcomplex.
CC {ECO:0000250|UniProtKB:Q15007}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:Q15007}.
CC Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q15007}.
CC -!- DEVELOPMENTAL STAGE: Maternally expressed from the 4-cell stage and
CC ubiquitously expressed through early embryogenesis, with enriched
CC expression in the brain region at 36 hpf (hours post fertilization).
CC {ECO:0000269|PubMed:24407421}.
CC -!- DISRUPTION PHENOTYPE: Multiple developmental defects at 24 hpf (hours
CC post fertilization) including smaller head and eyes, smaller brain
CC ventricle, and curved notochord. {ECO:0000269|PubMed:24407421}.
CC -!- SIMILARITY: Belongs to the fl(2)d family. {ECO:0000305}.
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DR EMBL; AL929182; CAI11704.1; -; Genomic_DNA.
DR EMBL; BC055544; AAH55544.1; -; mRNA.
DR RefSeq; NP_956147.1; NM_199853.1.
DR RefSeq; XP_009292974.1; XM_009294699.2.
DR AlphaFoldDB; Q7SXL7; -.
DR SMR; Q7SXL7; -.
DR STRING; 7955.ENSDARP00000062551; -.
DR PaxDb; Q7SXL7; -.
DR Ensembl; ENSDART00000062552; ENSDARP00000062551; ENSDARG00000042642.
DR GeneID; 334058; -.
DR KEGG; dre:334058; -.
DR CTD; 9589; -.
DR ZFIN; ZDB-GENE-030131-5990; wtap.
DR eggNOG; KOG2991; Eukaryota.
DR GeneTree; ENSGT00390000013931; -.
DR HOGENOM; CLU_044551_1_0_1; -.
DR InParanoid; Q7SXL7; -.
DR OMA; FPMSPVV; -.
DR OrthoDB; 907345at2759; -.
DR PhylomeDB; Q7SXL7; -.
DR TreeFam; TF325869; -.
DR Reactome; R-DRE-72203; Processing of Capped Intron-Containing Pre-mRNA.
DR PRO; PR:Q7SXL7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 20.
DR Bgee; ENSDARG00000042642; Expressed in mature ovarian follicle and 28 other tissues.
DR ExpressionAtlas; Q7SXL7; baseline.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; ISS:UniProtKB.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0080009; P:mRNA methylation; ISS:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0042981; P:regulation of apoptotic process; IGI:ZFIN.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR033757; WTAP.
DR PANTHER; PTHR15217; PTHR15217; 1.
DR Pfam; PF17098; Wtap; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Developmental protein; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome.
FT CHAIN 1..423
FT /note="Pre-mRNA-splicing regulator WTAP"
FT /id="PRO_0000308627"
FT REGION 234..423
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 234..305
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 392..423
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 423 AA; 46152 MW; A2600E687DAA229C CRC64;
MTNEEPLPKK VRLSESDMKT LTREELCTRW KQHETYVQML EAKYADLNSN DVTGLKESEE
KLKQQQQESA RRENILVMRL ATKEQEMQEC TTQIQYLKQV QQPSAAQLRS SMVDPAINLF
FLKMKAELEQ TKDKLEQAQN ELSAWKFTPD SQTGKKLMAK CRMLIQENQE LGRQLSQGRI
AQLEAELALQ KKYSEELKSS QDELNDFIIQ LDEEVEGMQS TILVLQQQLR ETRQQLSQMN
QTQGTSSGAG PSRTSPSTAS EPSTQSEPAN ASSSNVGKDC GRVSNGPSNG NSSQRGASGS
SLYREASSAD EDYPPSPSVS SPTHDGISKL SNHSEDAVSQ RGGEGYVTQL SAGYESVDSP
TGSETSVTQH SNDTDSNADS HEAAAVPKGS RTAGTRHSTQ NGLDSSAAAV ATNTSNASAG
SVL
