FL2D_DROPS
ID FL2D_DROPS Reviewed; 560 AA.
AC Q28XY0;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Pre-mRNA-splicing regulator female-lethal(2)D;
GN Name=fl(2)d; ORFNames=GA19508;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- FUNCTION: Associated component of the WMM complex, a complex that
CC mediates N6-methyladenosine (m6A) methylation of mRNAs, a modification
CC that plays a role in the efficiency of mRNA splicing and is required
CC for sex determination. Required for sex determination and dosage
CC compensation via Sxl alternative splicing: m6A methylation acts as a
CC key regulator of Sxl pre-mRNA and promotes female-specific alternative
CC splicing of Sxl, which determines female physiognomy. M6A methylation
CC is also required for neuronal functions. Required for proper inclusion
CC of regulated exons in Ubx transcripts, leading to isoforms Ia/b and
CC IIa/b. {ECO:0000250|UniProtKB:Q9Y091}.
CC -!- SUBUNIT: Component of the WMM complex, a N6-methyltransferase complex
CC composed of a catalytic subcomplex, named MAC, and of an associated
CC subcomplex, named MACOM. The MAC subcomplex is composed of Ime4/Mettl3
CC and Mettl14. The MACOM subcomplex is composed of fl(2)d, Flacc/Xio,
CC Hakai, vir, and, in some cases of nito. Interacts with vir and msk.
CC Part of a complex containing fl(2)d, Sxl and vir.
CC {ECO:0000250|UniProtKB:Q9Y091}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y091}.
CC -!- SIMILARITY: Belongs to the fl(2)d family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAL26186.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CM000071; EAL26186.2; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_001361607.3; XM_001361570.4.
DR AlphaFoldDB; Q28XY0; -.
DR SMR; Q28XY0; -.
DR STRING; 7237.FBpp0278439; -.
DR EnsemblMetazoa; FBtr0280001; FBpp0278439; FBgn0079504.
DR GeneID; 4805158; -.
DR KEGG; dpo:Dpse_GA19508; -.
DR eggNOG; KOG2991; Eukaryota.
DR InParanoid; Q28XY0; -.
DR Proteomes; UP000001819; Chromosome 3.
DR Bgee; FBgn0079504; Expressed in insect adult head and 2 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; IEA:EnsemblMetazoa.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0048749; P:compound eye development; IEA:EnsemblMetazoa.
DR GO; GO:0080009; P:mRNA methylation; IEA:EnsemblMetazoa.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000375; P:RNA splicing, via transesterification reactions; ISS:UniProtKB.
DR GO; GO:0007530; P:sex determination; IEA:EnsemblMetazoa.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR InterPro; IPR033757; WTAP.
DR PANTHER; PTHR15217; PTHR15217; 1.
DR Pfam; PF17098; Wtap; 1.
PE 3: Inferred from homology;
KW Coiled coil; Developmental protein; Differentiation; mRNA processing;
KW mRNA splicing; Nucleus; Reference proteome; Sexual differentiation.
FT CHAIN 1..560
FT /note="Pre-mRNA-splicing regulator female-lethal(2)D"
FT /id="PRO_0000308631"
FT REGION 35..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 371..413
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 541..560
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 87..218
FT /evidence="ECO:0000255"
FT COILED 262..324
FT /evidence="ECO:0000255"
FT COMPBIAS 87..102
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 327..350
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 374..388
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 398..412
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 543..560
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 560 AA; 61463 MW; 1D5D91EA393BE959 CRC64;
MSVAAMTMDD QRPLMNSYDK MSTAKYEQNI NLISSGASSG ISAGGVGGGG VPPSGPASPT
PSGSEESHHH PHHPHHSHHH QHHTPNQEQQ RQQLQQQEQQ HAAVAEAVAA AEQRQRLLED
EIETLKLEQV RMGQICADAQ RREKILMRRL ANKEQEFQDY VSQIAEYKAQ QAPTALALRT
ALLDPAVNLL FERLKKELKA TKTKLEETQN ELSAWKFTPD SNTGKRLMAK CRLLYQENEE
LGKMTSNGRL AKLETELAMQ KSFSEEVKKS QSELDDFLQE LDEDVEGMQS TILFLQQELK
TTRDRIQTLE KDNAQLKQAH TKDDTVAMAP PTTNGTNKMS PPQTPTPSTI GKLEPIDENV
ALESKAAHPD YLNGDTRSVN NNEQILPSSV PGEHILPDDV NNSNSNGNGN GNAARLARKR
NYEEETTTNM VTPTPTPVSY SVEEMTTVRE VSTPRILPPK KSKLRGMPTR RSSQLEEELH
AAATVAATVA TPLILDNSAT GMVSEEATAV AQPTLASVES GAVCPESTEA GVVAGPARIS
TRRRSVRMQQ NGAGPLDYST
