ID   FL2W_MAIZE              Reviewed;         262 AA.
AC   Q41896; A8HNM6;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 2.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Protein FLOURY 2 {ECO:0000303|PubMed:7624327};
DE   AltName: Full=22 kDa alpha-zein 16 {ECO:0000303|PubMed:11691845};
DE   AltName: Full=FLOURY-2 {ECO:0000303|PubMed:9159955};
DE   Flags: Precursor;
GN   Name=AZS22-16 {ECO:0000303|PubMed:11691845};
GN   Synonyms=FL2 {ECO:0000303|PubMed:7624327};
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577 {ECO:0000312|EMBL:AAA76580.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=cv. Wisconsin 64A; TISSUE=Leaf;
RX   PubMed=7624327; DOI=10.1073/pnas.92.15.6828;
RA   Coleman C.E., Lopes M.A., Gillikin J.W., Boston R.S., Larkins B.A.;
RT   "A defective signal peptide in the maize high-lysine mutant floury 2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:6828-6831(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 38-259.
RA   Feng L., Zhu J., Wang G., Xu Z., Song R.;
RT   "Expressional profiling of maize-zein super gene family during endosperm
RT   development.";
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   MUTAGENESIS OF ALA-21, AND SUBCELLULAR LOCATION.
RX   PubMed=9159955; DOI=10.1104/pp.114.1.345;
RA   Gillikin J.W., Zhang F., Coleman C.E., Bass H.W., Larkins B.A.,
RA   Boston R.S.;
RT   "A defective signal peptide tethers the floury-2 zein to the endoplasmic
RT   reticulum membrane.";
RL   Plant Physiol. 114:345-352(1997).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11691845; DOI=10.1101/gr.197301;
RA   Song R., Llaca V., Linton E., Messing J.;
RT   "Sequence, regulation, and evolution of the maize 22-kD alpha zein gene
RT   family.";
RL   Genome Res. 11:1817-1825(2001).
CC   -!- FUNCTION: Zeins are major seed storage proteins.
CC       {ECO:0000305|PubMed:7624327}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:9159955}. Note=The fl2 mutant remains anchored to
CC       endoplasmic reticulum membrane while the processed mature form
CC       localizes in the lumen. {ECO:0000269|PubMed:9159955}.
CC   -!- MISCELLANEOUS: The floury-2 (fl2) mutation is associated with the
CC       synthesis of a novel 24 kDa alpha-zein storage protein and an altered
CC       protein body morphology. The 24 kDa protein is expressed as a 22 kDa
CC       alpha-zein 16 with an uncleaved signal peptide, resulting in an
CC       enhanced Lys content of the grain and a soft texture of the endosperm.
CC       {ECO:0000305|PubMed:7624327}.
CC   -!- MISCELLANEOUS: Structurally, 22K and 19K zeins are composed of nine
CC       adjacent, topologically antiparallel helices clustered within a
CC       distorted cylinder. {ECO:0000250|UniProtKB:P04698}.
CC   -!- SIMILARITY: Belongs to the zein family. {ECO:0000305}.
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DR   EMBL; L34340; AAA76580.1; -; Genomic_DNA.
DR   EMBL; EU116495; ABV71977.1; -; mRNA.
DR   PIR; T04386; T04386.
DR   AlphaFoldDB; Q41896; -.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; Q41896; baseline and differential.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   InterPro; IPR002530; Zein.
DR   Pfam; PF01559; Zein; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endoplasmic reticulum; Membrane;
KW   Reference proteome; Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..262
FT                   /note="Protein FLOURY 2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5004231717"
FT   MUTAGEN         21
FT                   /note="A->V: In fl2; loss of processing of the signal
FT                   peptide and floury phenotype."
FT                   /evidence="ECO:0000269|PubMed:9159955"
SQ   SEQUENCE   262 AA;  28430 MW;  00532A8BA34FAD9C CRC64;
     MATKILALLA LLALLVSATN AFIIPQCSLA PSAIIPQFLP PVTSMGFEHP AVQAYRLQLV
     LAASALQQPI AQLQQQSLAH LTLQTIATQQ QQHFLPSLSH LAVVNPVAYL QQQLLASNPL
     ALANVATYQQ QQQLQQFMPA LSQLAMVNPA VYLQLLSSSP LAVGNAPTYL QQQLLQQIVP
     ALTHQLAMAN PATYLQQLLP FNQLAVSNSA AYLQQRQQLL NPLAVANPLV ATFLQQQQLL
     PYNQFSLMNP ALQQPIVGGA IF