AKR1_NEUCR
ID AKR1_NEUCR Reviewed; 729 AA.
AC Q7S3M5;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Palmitoyltransferase akr1;
DE EC=2.3.1.225;
DE AltName: Full=Ankyrin repeat-containing protein akr1;
DE AltName: Full=Palmitoyltransferase 1;
GN Name=ptr-1; Synonyms=akr1; ORFNames=NCU08218;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Palmitoyltransferase specific for casein kinase 1.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225;
CC -!- SUBCELLULAR LOCATION: Early endosome membrane; Multi-pass membrane
CC protein. Golgi apparatus membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC AKR/ZDHHC17 subfamily. {ECO:0000305}.
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DR EMBL; CM002238; EAA30072.3; -; Genomic_DNA.
DR RefSeq; XP_959308.3; XM_954215.3.
DR AlphaFoldDB; Q7S3M5; -.
DR SMR; Q7S3M5; -.
DR STRING; 5141.EFNCRP00000004752; -.
DR EnsemblFungi; EAA30072; EAA30072; NCU08218.
DR GeneID; 3875430; -.
DR KEGG; ncr:NCU08218; -.
DR VEuPathDB; FungiDB:NCU08218; -.
DR HOGENOM; CLU_012510_1_0_1; -.
DR InParanoid; Q7S3M5; -.
DR Proteomes; UP000001805; Chromosome 3, Linkage Group III.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016409; F:palmitoyltransferase activity; IBA:GO_Central.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0018345; P:protein palmitoylation; IBA:GO_Central.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF01529; DHHC; 1.
DR SMART; SM00248; ANK; 5.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 5.
DR PROSITE; PS50216; DHHC; 1.
PE 3: Inferred from homology;
KW Acyltransferase; ANK repeat; Endosome; Golgi apparatus; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Repeat; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..729
FT /note="Palmitoyltransferase akr1"
FT /id="PRO_0000212927"
FT TOPO_DOM 1..297
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 340..354
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 376..383
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 405..483
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..504
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 505..534
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 535..555
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 556..729
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 76..105
FT /note="ANK 1"
FT REPEAT 110..139
FT /note="ANK 2"
FT REPEAT 143..172
FT /note="ANK 3"
FT REPEAT 176..205
FT /note="ANK 4"
FT REPEAT 209..238
FT /note="ANK 5"
FT DOMAIN 440..490
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 587..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 709..729
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 470
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 729 AA; 80085 MW; 0F11B0993956E381 CRC64;
MVHHDGADAH AGHAAPAQPP MKSDTATPKL NSEVELGSLP SEAHNDIMQM ARVGDITGME
KLFAAGEYDA TYSDDEGITP LHWAAINNQY AMCKFLIDKG AEINKKGGES VATPLQWAAQ
RCHYYTVHLL LQHGADPLIT DSQGYNTLHI STFNGNVLLI VLLLHQGIPV DVEDAYGHTA
LMWSAYKGFP ACVDVFLRWG ASVHAKDEQG FTALHWALVK GSPGCIQKLI EYGADRFAKT
ANGKTPAITA QELNTVAAWQ KALDECGYDE HGNAIVPSWP GASYLLQDRR SFMTKFTFLW
PFVMVWATMV VMAGMPVFVG IPLGVLAGYA VQWVAQQVIA YAPPDMRQLQ KTPWMAGIFA
GSLFLCIMNW LLHIFGSTMF GQDSAVIPNL LFAFFISMTI WFYIRCMVDD PGFVPKMGGV
AEQKAVIDEL ISLWKFDESN FCVTCMIRTP LRSKHCRRCQ RCVAKHDHHC PWVYNCIGVN
NHRHFFFYLI NLTLSVVTYD WLTYRYLSTL SETASDECNI LAPSLCRIVN ADTYSLLTAI
WASLQLTWVS MLLFVQFVQV SSAMTTYENM HGIDNYSATS LNSSFTSTGA PLNPPSLPAP
GPSPAAGGAR HGGRHAHGHN HKQGFIKQWS RLLGVDAFIE TAAGRGATTG KGSKRNKRGN
PYSRGCVTNC KDFWCDPSPM FGKHENGAAV LGGVPVNYTD MYESPGVMTS GGGGRRRGGG
YESVAGEEV
