FLAS2_ARATH
ID FLAS2_ARATH Reviewed; 250 AA.
AC B2GVM7; Q84WQ4; Q9FFQ6;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Putative inactive flavonol synthase 2;
GN Name=FLS2; OrderedLocusNames=At5g63580; ORFNames=MBK5.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia;
RA De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-250.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18467451; DOI=10.1104/pp.108.117457;
RA Owens D.K., Alerding A.B., Crosby K.C., Bandara A.B., Westwood J.H.,
RA Winkel B.S.;
RT "Functional analysis of a predicted flavonol synthase gene family in
RT Arabidopsis.";
RL Plant Physiol. 147:1046-1061(2008).
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. Possesses a truncated
CC Fe2OG dioxygenase domain which lacks 1 iron binding site.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10451.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB005234; BAB10451.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97771.1; -; Genomic_DNA.
DR EMBL; BT031358; ACB88828.1; -; mRNA.
DR EMBL; BT002895; AAO22711.1; -; mRNA.
DR RefSeq; NP_201163.1; NM_125753.3.
DR AlphaFoldDB; B2GVM7; -.
DR SMR; B2GVM7; -.
DR STRING; 3702.AT5G63580.1; -.
DR PaxDb; B2GVM7; -.
DR EnsemblPlants; AT5G63580.1; AT5G63580.1; AT5G63580.
DR GeneID; 836477; -.
DR Gramene; AT5G63580.1; AT5G63580.1; AT5G63580.
DR KEGG; ath:AT5G63580; -.
DR Araport; AT5G63580; -.
DR TAIR; locus:2160564; AT5G63580.
DR eggNOG; KOG0143; Eukaryota.
DR HOGENOM; CLU_010119_16_2_1; -.
DR OrthoDB; 954853at2759; -.
DR PhylomeDB; B2GVM7; -.
DR BRENDA; 1.14.20.6; 399.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; B2GVM7; baseline and differential.
DR Genevisible; B2GVM7; AT.
DR GO; GO:0051213; F:dioxygenase activity; IBA:GO_Central.
DR GO; GO:0045431; F:flavonol synthase activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009813; P:flavonoid biosynthetic process; ISS:TAIR.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 5: Uncertain;
KW Iron; Metal-binding; Reference proteome.
FT CHAIN 1..250
FT /note="Putative inactive flavonol synthase 2"
FT /id="PRO_0000418024"
FT DOMAIN 171..250
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 195
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 197
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 250 AA; 28482 MW; A46B64A3DC5A8591 CRC64;
MEVERDQHIS PPSLMAKTIP IIDLSNLDEE LVAHAVVKGS EEWGIFHVVN HGIPMDLIQR
LKDVGTQFFE LPETEKKAVA KQDGSKDFEG YTTNLKYVKG EVWTENLFHR IWPPTCINFD
YWPKNPPQYR EVIEEYTKET KKLSERILGY LSEGLGLPSE ALIQGLGGES TEYVMRINNY
PPDPKPDLTL GVPEHTDIIG ITIIITNEVP GLQIFKDDHW LDVHYIPSSI TVNIGDQIMA
EQWKVQECVA
