FLA_ORYSJ
ID FLA_ORYSJ Reviewed; 382 AA.
AC Q2R4I5; Q0ISR9;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Inactive ubiquitin-specific protease 5 {ECO:0000303|PubMed:30603810};
DE Short=OsUBP5 {ECO:0000303|PubMed:30603810};
DE AltName: Full=Protein FLOWER AND LEAF COLOR ABERRANT {ECO:0000303|PubMed:30603810};
GN Name=UBP5 {ECO:0000303|PubMed:30603810};
GN Synonyms=FLA {ECO:0000303|PubMed:30603810};
GN OrderedLocusNames=Os11g0473200 {ECO:0000312|EMBL:BAT13996.1},
GN LOC_Os11g28360 {ECO:0000312|EMBL:ABA93659.2};
GN ORFNames=OSNPB_110473200 {ECO:0000312|EMBL:BAT13996.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP GENE FAMILY.
RX PubMed=30498503; DOI=10.3389/fpls.2018.01636;
RA Wang D.H., Song W., Wei S.W., Zheng Y.F., Chen Z.S., Han J.D., Zhang H.T.,
RA Luo J.C., Qin Y.M., Xu Z.H., Bai S.N.;
RT "Characterization of the ubiquitin C-terminal hydrolase and ubiquitin-
RT specific protease families in rice (Oryza sativa).";
RL Front. Plant Sci. 9:1636-1636(2018).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=30603810; DOI=10.1007/s00299-018-2368-4;
RA Ma X., Zhang J., Han B., Tang J., Cui D., Han L.;
RT "FLA, which encodes a homolog of UBP, is required for chlorophyll
RT accumulation and development of lemma and palea in rice.";
RL Plant Cell Rep. 38:321-331(2019).
CC -!- FUNCTION: Plays an important role in the development of floral organs
CC and chloroplasts (PubMed:30603810). Does not possess deubiquitinating
CC enzyme activity in vitro (PubMed:30603810).
CC {ECO:0000269|PubMed:30603810}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:30603810}.
CC -!- TISSUE SPECIFICITY: Widely expressed with the highest expression in
CC floral organs. {ECO:0000269|PubMed:30603810}.
CC -!- DISRUPTION PHENOTYPE: Abnormal floral organ and pollen development, and
CC leaf bleaching. {ECO:0000269|PubMed:30603810}.
CC -!- SIMILARITY: Belongs to the peptidase C19 family. {ECO:0000305}.
CC -!- CAUTION: Lacks the conserved Cys and His active sites that are
CC essential for the activity of deubiquitinating enzymes. Lacks ubiquitin
CC terminal hydrolase activity. {ECO:0000269|PubMed:30603810}.
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DR EMBL; DP000010; ABA93659.2; -; Genomic_DNA.
DR EMBL; AP008217; BAF28246.1; -; Genomic_DNA.
DR EMBL; AP014967; BAT13996.1; -; Genomic_DNA.
DR AlphaFoldDB; Q2R4I5; -.
DR SMR; Q2R4I5; -.
DR STRING; 4530.OS11T0473200-01; -.
DR MEROPS; C19.093; -.
DR PaxDb; Q2R4I5; -.
DR PRIDE; Q2R4I5; -.
DR EnsemblPlants; Os11t0473200-01; Os11t0473200-01; Os11g0473200.
DR Gramene; Os11t0473200-01; Os11t0473200-01; Os11g0473200.
DR eggNOG; KOG1870; Eukaryota.
DR HOGENOM; CLU_724410_0_0_1; -.
DR InParanoid; Q2R4I5; -.
DR OMA; YMIISES; -.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016579; P:protein deubiquitination; IEA:InterPro.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR Gene3D; 3.30.2230.10; -; 1.
DR InterPro; IPR035927; DUSP-like_sf.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR006615; Pept_C19_DUSP.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR Pfam; PF06337; DUSP; 1.
DR Pfam; PF00443; UCH; 1.
DR SMART; SM00695; DUSP; 1.
DR SUPFAM; SSF143791; SSF143791; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS51283; DUSP; 1.
DR PROSITE; PS00972; USP_1; 1.
DR PROSITE; PS50235; USP_3; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome.
FT CHAIN 1..382
FT /note="Inactive ubiquitin-specific protease 5"
FT /id="PRO_0000454381"
FT DOMAIN 16..141
FT /note="DUSP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00613"
FT DOMAIN 323..382
FT /note="USP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01035"
SQ SEQUENCE 382 AA; 42754 MW; 29F85746746952D0 CRC64;
MEMEMEMVVA VPSPEVPAEE ERALIRDITV AAEAHAKEGD TFFLITHRWW QSWIDYVIQD
LANSTNNGSH HHEHGSNVLR RPGAIDNTDL IDDTASEVSN MEIELHDTLV EGRDYILLPQ
QVWEKLHGWY GGGPTLPRKA INTGLSQTDL AIEVYPLRLQ LLLAPKGEQA VIRISKKDTV
GELHKKACEV FDLIPDEVCI WDYYGRTRHS LMDNLEKTLD DANIQMDQDI LVEVTTDANG
SLDGGCIGSI QENEYLERES TSLIADASKS GLSNENFASN NYTSRSYSSS LTQSQYLRSS
NGDLDNMHGT SAMITRGSPL GLTGLLNLGN TCFMNSAIQC LVHTPEFARY FREDYHREIN
WQNPLGMVVS TLSTSMALKP YV
