AKRP_ARATH
ID AKRP_ARATH Reviewed; 435 AA.
AC Q05753; Q3E852; Q8GYN6; Q940Y0;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 13-DEC-2002, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Ankyrin repeat domain-containing protein, chloroplastic;
DE Short=AKRP;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2036;
DE Flags: Precursor;
GN Name=AKRP; Synonyms=AKR, EMB2036; OrderedLocusNames=At5g66055;
GN ORFNames=K2A18.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION BY LIGHT.
RC STRAIN=cv. Landsberg erecta; TISSUE=Leaf;
RX PubMed=1281700; DOI=10.2307/3869500;
RA Zhang H., Scheirer D.C., Fowle W.H., Goodman H.M.;
RT "Expression of antisense or sense RNA of an ankyrin repeat-containing gene
RT blocks chloroplast differentiation in Arabidopsis.";
RL Plant Cell 4:1575-1588(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH EMB506,
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=17092312; DOI=10.1111/j.1365-313x.2006.02922.x;
RA Garcion C., Guilleminot J., Kroj T., Parcy F., Giraudat J., Devic M.;
RT "AKRP and EMB506 are two ankyrin repeat proteins essential for plastid
RT differentiation and plant development in Arabidopsis.";
RL Plant J. 48:895-906(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. C24;
RX PubMed=12232406; DOI=10.1104/pp.106.4.1261;
RA Zhang H., Wang J., Goodman H.M.;
RT "Expression of the Arabidopsis gene Akr coincides with chloroplast
RT development.";
RL Plant Physiol. 106:1261-1267(1994).
CC -!- FUNCTION: Involved in the initial differentiation of the proplastid
CC during the embryo development and in plastid differentiation linked to
CC cell differentiation, morphogenesis and organogenesis during the plant
CC life cycle.
CC -!- SUBUNIT: Interacts with EMB506. No homodimerization observed.
CC {ECO:0000269|PubMed:17092312}.
CC -!- INTERACTION:
CC Q05753; Q9SQK3: EMB506; NbExp=3; IntAct=EBI-2114020, EBI-2114010;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:17092312}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q05753-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q05753-2; Sequence=VSP_030158, VSP_030159;
CC -!- TISSUE SPECIFICITY: Expressed mainly in chloroplast-containing tissues.
CC Also detected in roots, stems, flower buds, developing siliques and dry
CC seeds. {ECO:0000269|PubMed:12232406, ECO:0000269|PubMed:17092312}.
CC -!- DEVELOPMENTAL STAGE: Highest expression occurs in four-day-old plants
CC and declines as plants develop further. {ECO:0000269|PubMed:12232406}.
CC -!- INDUCTION: By light. {ECO:0000269|PubMed:1281700}.
CC -!- DISRUPTION PHENOTYPE: Plants show a developmental arrest of the embryos
CC at the globular stage. {ECO:0000269|PubMed:17092312}.
CC -!- MISCELLANEOUS: [Isoform 1]: Found predominantly in leaves and
CC inflorescence stems.
CC -!- MISCELLANEOUS: [Isoform 2]: Found predominantly in flower buds and
CC siliques. Seems to be unable to interact with EMB506. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA32812.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M82883; AAA32812.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AB011474; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED98151.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98152.1; -; Genomic_DNA.
DR EMBL; AK117484; BAC42148.1; -; mRNA.
DR EMBL; AY052363; AAK96554.1; -; mRNA.
DR EMBL; BT001055; AAN46809.1; -; mRNA.
DR PIR; JQ1729; JQ1729.
DR RefSeq; NP_569027.2; NM_126003.3. [Q05753-1]
DR RefSeq; NP_975000.1; NM_203271.2. [Q05753-2]
DR PDB; 6JD6; X-ray; 2.20 A; B=37-435.
DR PDBsum; 6JD6; -.
DR AlphaFoldDB; Q05753; -.
DR SMR; Q05753; -.
DR BioGRID; 21979; 1.
DR IntAct; Q05753; 1.
DR STRING; 3702.AT5G66055.1; -.
DR iPTMnet; Q05753; -.
DR PaxDb; Q05753; -.
DR PRIDE; Q05753; -.
DR ProteomicsDB; 244891; -. [Q05753-1]
DR EnsemblPlants; AT5G66055.1; AT5G66055.1; AT5G66055. [Q05753-1]
DR EnsemblPlants; AT5G66055.2; AT5G66055.2; AT5G66055. [Q05753-2]
DR GeneID; 836737; -.
DR Gramene; AT5G66055.1; AT5G66055.1; AT5G66055. [Q05753-1]
DR Gramene; AT5G66055.2; AT5G66055.2; AT5G66055. [Q05753-2]
DR KEGG; ath:AT5G66055; -.
DR Araport; AT5G66055; -.
DR TAIR; locus:505006718; AT5G66055.
DR eggNOG; KOG0504; Eukaryota.
DR HOGENOM; CLU_048352_0_0_1; -.
DR InParanoid; Q05753; -.
DR OMA; EDHVIGD; -.
DR PhylomeDB; Q05753; -.
DR PRO; PR:Q05753; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q05753; baseline and differential.
DR Genevisible; Q05753; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR DisProt; DP02920; -.
DR Gene3D; 1.25.40.20; -; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF12796; Ank_2; 1.
DR SMART; SM00248; ANK; 5.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; ANK repeat; Chloroplast; Plastid;
KW Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..36
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 37..435
FT /note="Ankyrin repeat domain-containing protein,
FT chloroplastic"
FT /id="PRO_0000001621"
FT REPEAT 259..288
FT /note="ANK 1"
FT REPEAT 292..321
FT /note="ANK 2"
FT REPEAT 325..354
FT /note="ANK 3"
FT REPEAT 358..387
FT /note="ANK 4"
FT REPEAT 391..424
FT /note="ANK 5"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 175..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..196
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 358
FT /note="D -> V (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17092312"
FT /id="VSP_030158"
FT VAR_SEQ 360..435
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17092312"
FT /id="VSP_030159"
FT CONFLICT 16
FT /note="R -> G (in Ref. 1; AAA32812)"
FT /evidence="ECO:0000305"
FT CONFLICT 30
FT /note="A -> V (in Ref. 1; AAA32812)"
FT /evidence="ECO:0000305"
FT CONFLICT 73
FT /note="D -> E (in Ref. 1; AAA32812)"
FT /evidence="ECO:0000305"
FT CONFLICT 105
FT /note="L -> P (in Ref. 5; BAC42148)"
FT /evidence="ECO:0000305"
FT CONFLICT 146
FT /note="P -> Q (in Ref. 5; BAC42148)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="S -> F (in Ref. 1; AAA32812)"
FT /evidence="ECO:0000305"
FT CONFLICT 296
FT /note="V -> A (in Ref. 1; AAA32812)"
FT /evidence="ECO:0000305"
FT CONFLICT 406
FT /note="I -> T (in Ref. 1; AAA32812)"
FT /evidence="ECO:0000305"
FT HELIX 234..237
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 240..246
FT /evidence="ECO:0007829|PDB:6JD6"
FT TURN 253..256
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 263..269
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 273..279
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 296..302
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 306..314
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 329..335
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 339..347
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 362..369
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 372..380
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 395..399
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 406..417
FT /evidence="ECO:0007829|PDB:6JD6"
FT HELIX 418..421
FT /evidence="ECO:0007829|PDB:6JD6"
SQ SEQUENCE 435 AA; 48878 MW; DE306D487CC894EC CRC64;
MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA GDDFIVGDCL
VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI EPENLVPEEW RDIQAEVNLT
KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD
SGASSDGETA VSSPSERVAP KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK
EEKFMLNSRN PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA
IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA DINAQDRDGW
TPLHVAVQAR RSDIVKLLLI KGADIEVKNK DGLTPLGLCL YLGREIRTYE VMKLLKEFPL
SRHKKRLVTT DEDIE
