ID   FLDP_PSEAB              Reviewed;         184 AA.
AC   A0A0H2ZDT7;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 23.
DE   RecName: Full=Flavodoxin FldP {ECO:0000303|PubMed:24550745};
GN   Name=fldP {ECO:0000303|PubMed:24550745};
GN   Synonyms=wrbA {ECO:0000312|EMBL:ABJ12450.1};
GN   OrderedLocusNames=PA14_22540 {ECO:0000312|EMBL:ABJ12450.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   FUNCTION, COFACTOR, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=24550745; DOI=10.1371/journal.pgen.1004163;
RA   Moyano A.J., Tobares R.A., Rizzi Y.S., Krapp A.R., Mondotte J.A.,
RA   Bocco J.L., Saleh M.C., Carrillo N., Smania A.M.;
RT   "A long-chain flavodoxin protects Pseudomonas aeruginosa from oxidative
RT   stress and host bacterial clearance.";
RL   PLoS Genet. 10:E1004163-E1004163(2014).
CC   -!- FUNCTION: Flavodoxins are low-potential electron donors to a number of
CC       redox enzymes. FldP protects the cell from oxidative stress and
CC       reactive oxygen species (ROS) damage, thereby expanding the
CC       capabilities of P.aeruginosa to thrive in hostile environments, and
CC       contributes to bacterial survival within the host. In vitro, is able to
CC       mediate ferredoxin-NADP(H) reductase (FNR)-driven cytochrome c
CC       reduction. {ECO:0000269|PubMed:24550745}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000269|PubMed:24550745};
CC   -!- INDUCTION: Up-regulated by oxidative stress conditions through an OxyR-
CC       independent mechanism. Makes part of an operon that consists of
CC       PA14_22510 to PA14_22540. Is repressed by the LysR-like transcriptional
CC       regulator PA14_22550. {ECO:0000269|PubMed:24550745}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene accumulate higher
CC       intracellular ROS levels and exhibit decreased tolerance to H(2)O(2)
CC       toxicity compared to wild-type siblings. Inactivation of this gene also
CC       produces a moderate but significant decrease of 24% in the
CC       intracellular survival of P.aeruginosa in phagocytic cells. During in
CC       vivo infection of Drosophila melanogaster, flies that have been fed
CC       with the wild-type strain of P.aeruginosa die faster than those fed
CC       with the fldP deletion mutant. {ECO:0000269|PubMed:24550745}.
CC   -!- SIMILARITY: Belongs to the FldP flavodoxin family. {ECO:0000305}.
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DR   EMBL; CP000438; ABJ12450.1; -; Genomic_DNA.
DR   RefSeq; WP_003138419.1; NZ_CP034244.1.
DR   AlphaFoldDB; A0A0H2ZDT7; -.
DR   SMR; A0A0H2ZDT7; -.
DR   EnsemblBacteria; ABJ12450; ABJ12450; PA14_22540.
DR   KEGG; pau:PA14_22540; -.
DR   HOGENOM; CLU_051402_2_1_6; -.
DR   OMA; WVGTGMM; -.
DR   BioCyc; PAER208963:G1G74-1877-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0016655; F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor; IEA:UniProt.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR008254; Flavodoxin/NO_synth.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
DR   PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Electron transport; Flavoprotein; FMN; Stress response; Transport.
FT   CHAIN           1..184
FT                   /note="Flavodoxin FldP"
FT                   /id="PRO_0000442134"
FT   DOMAIN          4..176
FT                   /note="Flavodoxin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00088"
FT   BINDING         10..14
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00088"
FT   BINDING         91..147
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00088"
SQ   SEQUENCE   184 AA;  19522 MW;  F0E2679BB5E67559 CRC64;
     MSKAVVVYFS GYGHTKRVAQ AAAEGAQASL VEIDSEGNIP EAAWDLLDQA QSILFGAPTY
     MGSVPWQFKK FADATSRKWF VRQWQDKVFG GFTNSASLNG DKQVSLILMH TLASQHGGIW
     VSLGLAPANT SASSRADINN LGASVGALVQ SPSDADAQAI PSGDLETVKL YAARVARIGQ
     QLHA