ID   FLES_PSEAE              Reviewed;         402 AA.
AC   Q9I4N4;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Sensor protein kinase FleS {ECO:0000303|PubMed:7591148};
DE            EC=2.7.13.3 {ECO:0000250|UniProtKB:Q06067};
GN   Name=fleS; OrderedLocusNames=PA1098;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PAK;
RX   PubMed=7591148; DOI=10.1128/iai.63.12.4868-4876.1995;
RA   Ritchings B.W., Almira E.C., Lory S., Ramphal R.;
RT   "Cloning and phenotypic characterization of fleS and fleR, new response
RT   regulators of Pseudomonas aeruginosa which regulate motility and adhesion
RT   to mucin.";
RL   Infect. Immun. 63:4868-4876(1995).
RN   [3]
RP   FUNCTION.
RX   PubMed=30877999; DOI=10.1016/j.isci.2019.02.028;
RA   Kollaran A.M., Joge S., Kotian H.S., Badal D., Prakash D., Mishra A.,
RA   Varma M., Singh V.;
RT   "Context-Specific Requirement of Forty-Four Two-Component Loci in
RT   Pseudomonas aeruginosa Swarming.";
RL   IScience 13:305-317(2019).
CC   -!- FUNCTION: Member of the two-component regulatory system FleS/FleR that
CC       regulates the expression of multiple genes involved in flagellar
CC       synthesis, adhesion, swarming, motility and antibiotic resistance
CC       (PubMed:30877999, PubMed:7591148). May function as a membrane-
CC       associated protein kinase that phosphorylates FleR in response to
CC       environmental signals leading to activation of specific gene promoters
CC       (Probable). {ECO:0000269|PubMed:30877999, ECO:0000269|PubMed:7591148,
CC       ECO:0000305|PubMed:7591148}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3; Evidence={ECO:0000250|UniProtKB:Q06067};
CC   -!- DISRUPTION PHENOTYPE: Mutant shows severe swimming defect.
CC       {ECO:0000269|PubMed:30877999}.
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DR   EMBL; AE004091; AAG04487.1; -; Genomic_DNA.
DR   PIR; C83508; C83508.
DR   RefSeq; NP_249789.1; NC_002516.2.
DR   RefSeq; WP_003086450.1; NZ_QZGE01000006.1.
DR   AlphaFoldDB; Q9I4N4; -.
DR   SMR; Q9I4N4; -.
DR   STRING; 287.DR97_839; -.
DR   PaxDb; Q9I4N4; -.
DR   EnsemblBacteria; AAG04487; AAG04487; PA1098.
DR   GeneID; 878201; -.
DR   KEGG; pae:PA1098; -.
DR   PATRIC; fig|208964.12.peg.1137; -.
DR   PseudoCAP; PA1098; -.
DR   HOGENOM; CLU_000445_114_39_6; -.
DR   InParanoid; Q9I4N4; -.
DR   OMA; SEHARME; -.
DR   PhylomeDB; Q9I4N4; -.
DR   BioCyc; PAER208964:G1FZ6-1121-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IDA:PseudoCAP.
DR   CDD; cd00082; HisKA; 1.
DR   CDD; cd00130; PAS; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR000014; PAS.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   Pfam; PF13188; PAS_8; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SMART; SM00091; PAS; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
DR   PROSITE; PS50112; PAS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Transferase; Two-component regulatory system.
FT   CHAIN           1..402
FT                   /note="Sensor protein kinase FleS"
FT                   /id="PRO_0000449420"
FT   DOMAIN          188..393
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         191
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   402 AA;  43871 MW;  3D41AC9D28034861 CRC64;
     MQPALNAFTE QPADTAEATS RAGLEQAFAL FNQMSSQLSE SYSLLEERVT ELKGQLALVS
     AQRMEELAEK ERLANRLQSL LDLLPGGVIV IDAHGVVREA NPAALGLLGE PLVGMLWREV
     IARCFAPRED DGHEISLRDG RRLSIATRSL NGEPGQLILL NDLTDTRRLQ EQLARHERLS
     ALGRMVASLA HQIRTPLSAA LLYAGHLSEQ ALPTDQQQRF AGRLKERLHE LEHQVRDMLV
     FARGELPLTD RVAPKALFDS LRAAAEVHVQ GLQVRWQCEA RGGELLCNRD TLVGTVLNLV
     ENAIQACGPE LRLKVHLYAR ADSLRLSVSD NGPGMDPATL ARLGEPFFTT KTTGTGLGLA
     VVKAVARAHQ GQLQLRSRPG RGTCATLILP LIPAAPLSAI QE