位置:首页 > 蛋白库 > AKT61_ALTAL
AKT61_ALTAL
ID   AKT61_ALTAL             Reviewed;         298 AA.
AC   V5XZU2;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   19-FEB-2014, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Enoyl-CoA hydratase AKT6-1 {ECO:0000303|PubMed:24611558};
DE            EC=4.2.1.- {ECO:0000305|PubMed:24611558};
DE   AltName: Full=AK-toxin biosynthesis protein 6-1 {ECO:0000303|PubMed:24611558};
GN   Name=AKT6-1 {ECO:0000303|PubMed:24611558};
OS   Alternaria alternata (Alternaria rot fungus) (Torula alternata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC   Alternaria sect. Alternaria; Alternaria alternata complex.
OX   NCBI_TaxID=5599;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC   STRAIN=15A;
RX   PubMed=24611558; DOI=10.1111/nph.12754;
RA   Takaoka S., Kurata M., Harimoto Y., Hatta R., Yamamoto M., Akimitsu K.,
RA   Tsuge T.;
RT   "Complex regulation of secondary metabolism controlling pathogenicity in
RT   the phytopathogenic fungus Alternaria alternata.";
RL   New Phytol. 202:1297-1309(2014).
RN   [2]
RP   FUNCTION.
RC   STRAIN=15A;
RX   PubMed=10432635; DOI=10.1094/mpmi.1999.12.8.691;
RA   Tanaka A., Shiotani H., Yamamoto M., Tsuge T.;
RT   "Insertional mutagenesis and cloning of the genes required for biosynthesis
RT   of the host-specific AK-toxin in the Japanese pear pathotype of Alternaria
RT   alternata.";
RL   Mol. Plant Microbe Interact. 12:691-702(1999).
RN   [3]
RP   FUNCTION.
RX   PubMed=10975654; DOI=10.1094/mpmi.2000.13.9.975;
RA   Tanaka A., Tsuge T.;
RT   "Structural and functional complexity of the genomic region controlling AK-
RT   toxin biosynthesis and pathogenicity in the Japanese pear pathotype of
RT   Alternaria alternata.";
RL   Mol. Plant Microbe Interact. 13:975-986(2000).
RN   [4]
RP   FUNCTION.
RX   PubMed=20348386; DOI=10.1128/ec.00369-09;
RA   Imazaki A., Tanaka A., Harimoto Y., Yamamoto M., Akimitsu K., Park P.,
RA   Tsuge T.;
RT   "Contribution of peroxisomes to secondary metabolism and pathogenicity in
RT   the fungal plant pathogen Alternaria alternata.";
RL   Eukaryot. Cell 9:682-694(2010).
RN   [5]
RP   REVIEW ON HOST-SELECTIVE TOXINS.
RX   PubMed=22846083; DOI=10.1111/j.1574-6976.2012.00350.x;
RA   Tsuge T., Harimoto Y., Akimitsu K., Ohtani K., Kodama M., Akagi Y.,
RA   Egusa M., Yamamoto M., Otani H.;
RT   "Host-selective toxins produced by the plant pathogenic fungus Alternaria
RT   alternata.";
RL   FEMS Microbiol. Rev. 37:44-66(2013).
CC   -!- FUNCTION: Enoyl-CoA hydratase; part of the gene clusters that mediate
CC       the biosynthesis of the host-selective toxins (HSTs) AK-toxins
CC       responsible for Japanese pear black spot disease by the Japanese pear
CC       pathotype (PubMed:24611558). AK-toxins are esters of 9,10-epoxy 8-
CC       hydroxy 9-methyldecatrienoic acid (EDA) (PubMed:22846083). On cellular
CC       level, AK-toxins affect plasma membrane of susceptible cells and cause
CC       a sudden increase in loss of K(+) after a few minutes of toxin
CC       treatment (PubMed:22846083). The acyl-CoA ligase AKT1, the hydrolase
CC       AKT2 and enoyl-CoA hydratase AKT3 are all involved in the biosynthesis
CC       of the AK-, AF- and ACT-toxin common 9,10-epoxy-8-hydroxy-9-methyl-
CC       decatrienoic acid (EDA) structural moiety (PubMed:10432635,
CC       PubMed:10975654, PubMed:22846083). Part of the EDA biosynthesis occurs
CC       in the peroxisome since these 3 enzymes are localized in peroxisomes
CC       (PubMed:20348386). The exact roles of the 3 enzymes, as well as of
CC       additional AK-toxin clusters enzymes, including AKT4, AKT6 and AKTS1,
CC       have still to be elucidated (PubMed:10432635, PubMed:10975654,
CC       PubMed:22846083). The Cytochrome P450 monooxygenase AKT7 on the other
CC       side functions to limit production of EDA and AK-toxin, probably via
CC       the catalysis of a side reaction of EDA or its precursor
CC       (PubMed:24611558). {ECO:0000269|PubMed:10432635,
CC       ECO:0000269|PubMed:10975654, ECO:0000269|PubMed:20348386,
CC       ECO:0000269|PubMed:24611558, ECO:0000303|PubMed:22846083}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:24611558}.
CC   -!- MISCELLANEOUS: Gene clusters encoding host-selective toxins (HSTs) are
CC       localized on conditionally dispensable chromosomes (CDCs), also called
CC       supernumerary chromosomes, where they are present in multiple copies
CC       (PubMed:10975654). The CDCs are not essential for saprophytic growth
CC       but controls host-selective pathogenicity (PubMed:10975654).
CC       {ECO:0000269|PubMed:10975654}.
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB872924; BAO10617.1; -; Genomic_DNA.
DR   AlphaFoldDB; V5XZU2; -.
DR   SMR; V5XZU2; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Lyase; Virulence.
FT   CHAIN           1..298
FT                   /note="Enoyl-CoA hydratase AKT6-1"
FT                   /id="PRO_0000444835"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   298 AA;  32611 MW;  10140BE17E17C69F CRC64;
     MTFSTTKSVA MSPDDDAPSF DINSSRRLMS HSEVESRGNG YEVLQQAGTV RILLSRPERG
     NALSLSLAKD LTQLFQTFSS QHSVHRIVLT GKGKYFCSGM DLGEELYEDA TERCLALQDL
     FGAIDACPKT TVAAINGPAF GGGVGLAFVC DIRVAVSTSF FCLSEVKLGL CPATVSRFII
     REWGVSLARM AMLTARKIHP QALQEMGVLH AVALDEEALK VVTENLLDDL GFAAPQASAW
     CKALTRKTRN GNSDHDQFAR QISETMVAPG SESEYGVAQF RRGRKNIRWE QAECLHTR
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024