ID   FLGJ_ECO57              Reviewed;         313 AA.
AC   P58231;
DT   14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   14-AUG-2001, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Peptidoglycan hydrolase FlgJ;
DE            EC=3.2.1.-;
DE   AltName: Full=Muramidase FlgJ;
GN   Name=flgJ; Synonyms=fla FX, flaZ; OrderedLocusNames=Z1719, ECs1459;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Flagellum-specific muramidase which hydrolyzes the
CC       peptidoglycan layer to assemble the rod structure in the periplasmic
CC       space. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: Probably exported via the flagellum-specific export
CC       pathway.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the FlgJ family.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the glycosyl
CC       hydrolase 73 family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG55827.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34882.1; -; Genomic_DNA.
DR   PIR; C90811; C90811.
DR   PIR; G85670; G85670.
DR   RefSeq; NP_309486.1; NC_002695.1.
DR   RefSeq; WP_001301817.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P58231; -.
DR   SMR; P58231; -.
DR   STRING; 155864.EDL933_1658; -.
DR   CAZy; GH73; Glycoside Hydrolase Family 73.
DR   EnsemblBacteria; AAG55827; AAG55827; Z1719.
DR   EnsemblBacteria; BAB34882; BAB34882; ECs_1459.
DR   GeneID; 912388; -.
DR   KEGG; ece:Z1719; -.
DR   KEGG; ecs:ECs_1459; -.
DR   PATRIC; fig|386585.9.peg.1560; -.
DR   eggNOG; COG1705; Bacteria.
DR   eggNOG; COG3951; Bacteria.
DR   HOGENOM; CLU_013771_3_0_6; -.
DR   OMA; EGVFVQM; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004040; F:amidase activity; IEA:InterPro.
DR   GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR   GO; GO:0044780; P:bacterial-type flagellum assembly; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR019301; Flagellar_prot_FlgJ_N.
DR   InterPro; IPR013377; FlaJ.
DR   InterPro; IPR002901; MGlyc_endo_b_GlcNAc-like_dom.
DR   Pfam; PF01832; Glucosaminidase; 1.
DR   Pfam; PF10135; Rod-binding; 1.
DR   SMART; SM00047; LYZ2; 1.
DR   TIGRFAMs; TIGR02541; flagell_FlgJ; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum biogenesis; Cell wall biogenesis/degradation;
KW   Glycosidase; Hydrolase; Periplasm; Reference proteome.
FT   CHAIN           1..313
FT                   /note="Peptidoglycan hydrolase FlgJ"
FT                   /id="PRO_0000165706"
FT   REGION          148..313
FT                   /note="Catalytic"
FT   ACT_SITE        220
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        245
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   313 AA;  34535 MW;  CEB3D7E9D05C73AA CRC64;
     MISDSKLLAS AAWDAQSLNE LKAKASEDPA ANIRPVARQV EGMFVQMMLK SMRDALPKDG
     LFSSEHTRLY TSMYDQQIAQ QMTTGKGLGL AEMMVKQMTP EQPLPEESTP AAPMKFPLET
     VVRYQNQALS QLVQKAVPRN YDDSLPGDSK AFLAQLSLPA QLASQQSGVP HHLILAQAAL
     ESGWGQRQIR RENGEPSYNL FGVKASGNWK GPVTEITTTE YENGEAKKVK AKFRVYSSYL
     EALSDYVGLL TRNPRYAAVT TAASAEQGAQ ALQDAGYATD PHYARKLTNM IQQMKSISDK
     VSKTYSMNID NLF