ID   FLGL_ECOLI              Reviewed;         317 AA.
AC   P29744;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 2.
DT   03-AUG-2022, entry version 141.
DE   RecName: Full=Flagellar hook-associated protein 3;
DE            Short=HAP3;
DE   AltName: Full=Hook-filament junction protein;
GN   Name=flgL; Synonyms=flaT, flaU; OrderedLocusNames=b1083, JW1070;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / CS520;
RX   PubMed=8158647; DOI=10.1006/jmbi.1994.1279;
RA   Fahrner K.A., Block S.M., Krishnaswamy S., Parkinson J.S., Berg H.C.;
RT   "A mutant hook-associated protein (HAP3) facilitates torsionally induced
RT   transformations of the flagellar filament of Escherichia coli.";
RL   J. Mol. Biol. 238:173-186(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 292-317.
RC   STRAIN=K12;
RX   PubMed=1447789; DOI=10.1016/0022-2836(92)90489-7;
RA   Casaregola S., Jacq A., Laoudj D., McGurk G., Margarson S., Tempete M.,
RA   Norris V., Holland I.B.;
RT   "Cloning and analysis of the entire Escherichia coli ams gene. ams is
RT   identical to hmp1 and encodes a 114 kDa protein that migrates as a 180 kDa
RT   protein.";
RL   J. Mol. Biol. 228:30-40(1992).
CC   -!- SUBCELLULAR LOCATION: Secreted. Bacterial flagellum.
CC   -!- SIMILARITY: Belongs to the bacterial flagellin family. {ECO:0000305}.
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DR   EMBL; U02514; AAA18322.1; -; Unassigned_DNA.
DR   EMBL; U00096; AAC74167.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35892.1; -; Genomic_DNA.
DR   EMBL; X67470; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; S44022; S44022.
DR   RefSeq; NP_415601.1; NC_000913.3.
DR   RefSeq; WP_001212782.1; NZ_SSZK01000053.1.
DR   AlphaFoldDB; P29744; -.
DR   SMR; P29744; -.
DR   BioGRID; 4261022; 6.
DR   IntAct; P29744; 7.
DR   STRING; 511145.b1083; -.
DR   SWISS-2DPAGE; P29744; -.
DR   PaxDb; P29744; -.
DR   PRIDE; P29744; -.
DR   EnsemblBacteria; AAC74167; AAC74167; b1083.
DR   EnsemblBacteria; BAA35892; BAA35892; BAA35892.
DR   GeneID; 945646; -.
DR   KEGG; ecj:JW1070; -.
DR   KEGG; eco:b1083; -.
DR   PATRIC; fig|1411691.4.peg.1185; -.
DR   EchoBASE; EB1507; -.
DR   eggNOG; COG1344; Bacteria.
DR   HOGENOM; CLU_024437_5_0_6; -.
DR   InParanoid; P29744; -.
DR   OMA; MRVTQGM; -.
DR   PhylomeDB; P29744; -.
DR   BioCyc; EcoCyc:EG11545-MON; -.
DR   PRO; PR:P29744; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009424; C:bacterial-type flagellum hook; IEA:InterPro.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0071973; P:bacterial-type flagellum-dependent cell motility; IEA:InterPro.
DR   InterPro; IPR013384; Flagell_FlgL.
DR   InterPro; IPR001492; Flagellin.
DR   InterPro; IPR001029; Flagellin_N.
DR   PANTHER; PTHR42792; PTHR42792; 2.
DR   Pfam; PF00669; Flagellin_N; 1.
DR   TIGRFAMs; TIGR02550; flagell_flgL; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum; Reference proteome; Secreted.
FT   CHAIN           1..317
FT                   /note="Flagellar hook-associated protein 3"
FT                   /id="PRO_0000182661"
FT   VARIANT         168
FT                   /note="R -> C (in HAP3; exhibits impaired swimming only
FT                   when in contact with a solid surface or a semisolid
FT                   matrix)"
SQ   SEQUENCE   317 AA;  34281 MW;  8B947F26DB27FAFD CRC64;
     MRFSTQMMYQ QNMRGITNSQ AEWMKYGEQM STGKRVVNPS DDPIAASQAV VLSQAQAQNS
     QYTLARTFAT QKVSLEESVL SQVTTAIQNA QEKIVYASNG TLSDDDRASL ATDIQGLRDQ
     LLNLANTTDG NGRYIFAGYK TETAPFSEEK GKYVGGAESI KQQVDASRSM VIGHTGDKIF
     DSITSNAVAE PDGSASETNL FAMLDSAIAA LKTPVADSEA DKETAAAALD KTNRGLKNSL
     NNVLTVRAEL GTQLNELESL DSLGSDRALG QTQQMSDLVD VDWNATISSY IMQQTALQAS
     YKAFTDMQGL SLFQLSK