ID   FLGM_ECOLI              Reviewed;          97 AA.
AC   P0AEM4; P43532;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Negative regulator of flagellin synthesis;
DE   AltName: Full=Anti-sigma-28 factor;
GN   Name=flgM; OrderedLocusNames=b1071, JW1058;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Mytelka D.S., Chamberlin M.J.;
RT   "Regulation of class II motility genes in Escherichia coli.";
RL   Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   STRUCTURE BY NMR.
RX   PubMed=9095196; DOI=10.1038/nsb0497-285;
RA   Daughdrill G.W., Chadsey M.S., Karlinsey J.E., Hughes K.T., Dahlquist F.W.;
RT   "The C-terminal half of the anti-sigma factor, FlgM, becomes structured
RT   when bound to its target, sigma 28.";
RL   Nat. Struct. Biol. 4:285-291(1997).
CC   -!- FUNCTION: Responsible for the coupling of flagellin expression to
CC       flagellar assembly by preventing expression of the flagellin genes when
CC       a component of the middle class of proteins is defective. It negatively
CC       regulates flagellar genes by inhibiting the activity of FliA by
CC       directly binding to FliA.
CC   -!- INTERACTION:
CC       P0AEM4; P0AEM6: fliA; NbExp=4; IntAct=EBI-1121952, EBI-549807;
CC   -!- SIMILARITY: Belongs to the FlgM family. {ECO:0000305}.
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DR   EMBL; U19773; AAA61762.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74155.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35879.1; -; Genomic_DNA.
DR   PIR; D64850; D64850.
DR   RefSeq; NP_415589.1; NC_000913.3.
DR   RefSeq; WP_000020880.1; NZ_STEB01000016.1.
DR   AlphaFoldDB; P0AEM4; -.
DR   SMR; P0AEM4; -.
DR   BioGRID; 851026; 1.
DR   IntAct; P0AEM4; 13.
DR   STRING; 511145.b1071; -.
DR   jPOST; P0AEM4; -.
DR   PaxDb; P0AEM4; -.
DR   PRIDE; P0AEM4; -.
DR   EnsemblBacteria; AAC74155; AAC74155; b1071.
DR   EnsemblBacteria; BAA35879; BAA35879; BAA35879.
DR   GeneID; 66670662; -.
DR   GeneID; 946684; -.
DR   KEGG; ecj:JW1058; -.
DR   KEGG; eco:b1071; -.
DR   PATRIC; fig|1411691.4.peg.1197; -.
DR   EchoBASE; EB2530; -.
DR   eggNOG; COG2747; Bacteria.
DR   HOGENOM; CLU_149304_2_2_6; -.
DR   OMA; QSKLMQP; -.
DR   PhylomeDB; P0AEM4; -.
DR   BioCyc; EcoCyc:G369-MON; -.
DR   PRO; PR:P0AEM4; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016989; F:sigma factor antagonist activity; IMP:EcoCyc.
DR   GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR   GO; GO:0045861; P:negative regulation of proteolysis; IMP:EcoCyc.
DR   InterPro; IPR035890; Anti-sigma-28_factor_FlgM_sf.
DR   InterPro; IPR007412; FlgM.
DR   InterPro; IPR031316; FlgM_C.
DR   Pfam; PF04316; FlgM; 1.
DR   SUPFAM; SSF101498; SSF101498; 1.
DR   TIGRFAMs; TIGR03824; FlgM_jcvi; 1.
PE   1: Evidence at protein level;
KW   Bacterial flagellum biogenesis; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..97
FT                   /note="Negative regulator of flagellin synthesis"
FT                   /id="PRO_0000087283"
FT   REGION          1..39
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   97 AA;  10341 MW;  367D6F63324BF75D CRC64;
     MSIDRTSPLK PVSTVQPRET TDAPVTNSRA AKTTASTSTS VTLSDAQAKL MQPGSSDINL
     ERVEALKLAI RNGELKMDTG KIADALINEA QQDLQSN