ID   FLGM_SALTY              Reviewed;          97 AA.
AC   P26477;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Negative regulator of flagellin synthesis;
DE   AltName: Full=Anti-sigma-28 factor;
GN   Name=flgM; Synonyms=flgR, mviS; OrderedLocusNames=STM1172;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=1655712; DOI=10.1128/jb.173.20.6453-6459.1991;
RA   Gillen K.L., Hughes K.T.;
RT   "Molecular characterization of flgM, a gene encoding a negative regulator
RT   of flagellin synthesis in Salmonella typhimurium.";
RL   J. Bacteriol. 173:6453-6459(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=8288531; DOI=10.1128/jb.176.2.368-377.1994;
RA   Schmitt C.K., Darnell S.C., Tesh V.L., Stocker B.A.D., O'Brien A.D.;
RT   "Mutation of flgM attenuates virulence of Salmonella typhimurium, and
RT   mutation of fliA represses the attenuated phenotype.";
RL   J. Bacteriol. 176:368-377(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=8200538; DOI=10.1016/0378-1119(94)90603-3;
RA   Kutsukake K., Okada T., Yokoseki T., Iino T.;
RT   "Sequence analysis of the flgA gene and its adjacent region in Salmonella
RT   typhimurium, and identification of another flagellar gene, flgN.";
RL   Gene 143:49-54(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=8552046; DOI=10.1007/bf00287103;
RA   Iyoda S., Kutsukake K.;
RT   "Molecular dissection of the flagellum-specific anti-sigma factor, FlgM, of
RT   Salmonella typhimurium.";
RL   Mol. Gen. Genet. 249:417-424(1995).
CC   -!- FUNCTION: Responsible for the coupling of flagellin expression to
CC       flagellar assembly by preventing expression of the flagellin genes when
CC       a component of the middle class of proteins is defective. It negatively
CC       regulates flagellar genes by inhibiting the activity of FliA by
CC       directly binding to FliA.
CC   -!- INTERACTION:
CC       P26477; P0A2E8: fliA; NbExp=4; IntAct=EBI-10687647, EBI-10687707;
CC       P26477; P26609: fliS; NbExp=6; IntAct=EBI-10687647, EBI-2011519;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Note=After completion of the hook-
CC       basal body structure, it is exported out of the cell through the
CC       completely assembled hook-basal body structures.
CC   -!- DOMAIN: The N-terminal domain is essential for export and the C-
CC       terminal domain possess the FliA binding site.
CC   -!- SIMILARITY: Belongs to the FlgM family. {ECO:0000305}.
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DR   EMBL; M74222; AAA27075.1; -; Genomic_DNA.
DR   EMBL; U03631; AAC38036.1; -; Unassigned_DNA.
DR   EMBL; D25292; BAA04978.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL20102.1; -; Genomic_DNA.
DR   PIR; A41046; A41046.
DR   RefSeq; NP_460143.1; NC_003197.2.
DR   RefSeq; WP_000020893.1; NC_003197.2.
DR   AlphaFoldDB; P26477; -.
DR   SMR; P26477; -.
DR   IntAct; P26477; 2.
DR   STRING; 99287.STM1172; -.
DR   PaxDb; P26477; -.
DR   EnsemblBacteria; AAL20102; AAL20102; STM1172.
DR   GeneID; 1252690; -.
DR   KEGG; stm:STM1172; -.
DR   PATRIC; fig|99287.12.peg.1240; -.
DR   HOGENOM; CLU_149304_2_2_6; -.
DR   OMA; QSKLMQP; -.
DR   PhylomeDB; P26477; -.
DR   BioCyc; SENT99287:STM1172-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   DisProt; DP00027; -.
DR   InterPro; IPR035890; Anti-sigma-28_factor_FlgM_sf.
DR   InterPro; IPR007412; FlgM.
DR   InterPro; IPR031316; FlgM_C.
DR   Pfam; PF04316; FlgM; 1.
DR   SUPFAM; SSF101498; SSF101498; 1.
DR   TIGRFAMs; TIGR03824; FlgM_jcvi; 1.
PE   1: Evidence at protein level;
KW   Bacterial flagellum biogenesis; Cytoplasm; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..97
FT                   /note="Negative regulator of flagellin synthesis"
FT                   /id="PRO_0000087286"
FT   REGION          1..40
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   97 AA;  10568 MW;  78CC1779935E7E64 CRC64;
     MSIDRTSPLK PVSTVQTRET SDTPVQKTRQ EKTSAATSAS VTLSDAQAKL MQPGVSDINM
     ERVEALKTAI RNGELKMDTG KIADSLIREA QSYLQSK