ID   AKTIP_RAT               Reviewed;         292 AA.
AC   Q5FVH4;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=AKT-interacting protein;
DE   AltName: Full=Fused toes protein homolog;
GN   Name=Aktip; Synonyms=Fts;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Component of the FTS/Hook/FHIP complex (FHF complex). The FHF
CC       complex may function to promote vesicle trafficking and/or fusion via
CC       the homotypic vesicular protein sorting complex (the HOPS complex).
CC       Regulates apoptosis by enhancing phosphorylation and activation of
CC       AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling
CC       cascade. FHF complex promotes the distribution of AP-4 complex to the
CC       perinuclear area of the cell. {ECO:0000250|UniProtKB:Q9H8T0}.
CC   -!- SUBUNIT: Component of the FTS/Hook/FHIP complex (FHF complex), composed
CC       of AKTIP/FTS, FHIP1B, and one or more members of the Hook family of
CC       proteins HOOK1, HOOK2, and HOOK3. Interacts directly with HOOK1, HOOK2
CC       and HOOK3. The FHF complex associates with the homotypic vesicular
CC       sorting complex (the HOPS complex). Also interacts with AKT1. May
CC       interact with FHIP1A. {ECO:0000250|UniProtKB:Q9H8T0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9H8T0}. Cell
CC       membrane {ECO:0000250|UniProtKB:Q9H8T0}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9H8T0}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. FTS
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- CAUTION: Lacks the conserved Cys residue necessary for ubiquitin-
CC       conjugating enzyme E2 activity. {ECO:0000305}.
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DR   EMBL; BC089985; AAH89985.1; -; mRNA.
DR   RefSeq; NP_001011926.1; NM_001011926.1.
DR   AlphaFoldDB; Q5FVH4; -.
DR   SMR; Q5FVH4; -.
DR   STRING; 10116.ENSRNOP00000057588; -.
DR   PhosphoSitePlus; Q5FVH4; -.
DR   PaxDb; Q5FVH4; -.
DR   PRIDE; Q5FVH4; -.
DR   GeneID; 291906; -.
DR   KEGG; rno:291906; -.
DR   CTD; 64400; -.
DR   RGD; 1311170; Aktip.
DR   VEuPathDB; HostDB:ENSRNOG00000011956; -.
DR   eggNOG; KOG0429; Eukaryota.
DR   HOGENOM; CLU_083049_0_0_1; -.
DR   InParanoid; Q5FVH4; -.
DR   OMA; WGFPEWR; -.
DR   OrthoDB; 1226125at2759; -.
DR   PhylomeDB; Q5FVH4; -.
DR   TreeFam; TF314386; -.
DR   PRO; PR:Q5FVH4; -.
DR   Proteomes; UP000002494; Chromosome 19.
DR   Bgee; ENSRNOG00000011956; Expressed in duodenum and 20 other tissues.
DR   Genevisible; Q5FVH4; RN.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0070695; C:FHF complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0045022; P:early endosome to late endosome transport; ISS:UniProtKB.
DR   GO; GO:0007032; P:endosome organization; ISS:UniProtKB.
DR   GO; GO:0008333; P:endosome to lysosome transport; ISS:UniProtKB.
DR   GO; GO:0007040; P:lysosome organization; ISS:UniProtKB.
DR   GO; GO:0032092; P:positive regulation of protein binding; ISO:RGD.
DR   GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:RGD.
DR   GO; GO:1905719; P:protein localization to perinuclear region of cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   1: Evidence at protein level;
KW   Apoptosis; Cell membrane; Cytoplasm; Membrane; Phosphoprotein;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..292
FT                   /note="AKT-interacting protein"
FT                   /id="PRO_0000379018"
FT   DOMAIN          74..222
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   REGION          1..64
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         30
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H8T0"
SQ   SEQUENCE   292 AA;  32992 MW;  9F07F873B69A15EA CRC64;
     MNPLWSMSSG SVRKRAEGEE KTLAGDVKTS PPRSAPKKQL PSIPKNALPI SKPTSPAPAA
     QSTNGTHASY GPFYLEYSLL AEFTMVVKQK LPGVYVQPSY RSALVWFGVI FIRHGLYQDG
     VFKFTVYIPD NYPDGDCPRL LFDIPVFHPL VDPTSGELDV KRAFAKWRRN HNHIWQVLMY
     ARRVFYKIDT TSPLNPEAAV LYEKDIQLFK SKVVDSVKVC TARLFDQPKI EDPYAISFSP
     WNPSVHDEAR EKMLTQKKPD EQHNKSVHVA GLSWVKPGSV QPFSKEEKTV AT