FLH1_CAEEL
ID FLH1_CAEEL Reviewed; 507 AA.
AC Q9XWR1; A7DTF1; D3NQA3;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=FLYWCH transcription factor 1 {ECO:0000303|PubMed:18794349};
GN Name=flh-1 {ECO:0000312|WormBase:Y11D7A.12a};
GN ORFNames=Y11D7A.12 {ECO:0000312|WormBase:Y11D7A.12a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=18794349; DOI=10.1101/gad.1678808;
RA Ow M.C., Martinez N.J., Olsen P.H., Silverman H.S., Barrasa M.I.,
RA Conradt B., Walhout A.J., Ambros V.;
RT "The FLYWCH transcription factors FLH-1, FLH-2, and FLH-3 repress embryonic
RT expression of microRNA genes in C. elegans.";
RL Genes Dev. 22:2520-2534(2008).
CC -!- FUNCTION: Probable transcription factor (PubMed:18794349). Binds to the
CC DNA sequence motif 5'-[AG]GGCGCCG-3' in the promoters of target genes,
CC including micro-RNA genes, in order to repress expression, and acting
CC redundantly with flh-2 (PubMed:18794349).
CC {ECO:0000269|PubMed:18794349}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a {ECO:0000312|WormBase:Y11D7A.12a};
CC IsoId=Q9XWR1-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:Y11D7A.12b};
CC IsoId=Q9XWR1-2; Sequence=VSP_061184;
CC Name=c {ECO:0000312|WormBase:Y11D7A.12c};
CC IsoId=Q9XWR1-3; Sequence=VSP_061183;
CC -!- DEVELOPMENTAL STAGE: Expressed during embryogenesis and adulthood (at
CC protein level) (PubMed:18794349). Expressed in most cells starting at
CC gastrulation and diminishing by the L1 larval stage; however, also
CC expressed in L4 larval stage and adults (PubMed:18794349).
CC {ECO:0000269|PubMed:18794349}.
CC -!- DISRUPTION PHENOTYPE: Knockout, in a flh-2 mutant background, causes
CC poor coordination, egg-laying defects and dumpy appearance
CC (PubMed:18794349). Causes a ninefold to 10-fold decrease in lin-14
CC level in embryos and about 2-fold increase in the levels of micro-RNAs
CC lin-4 and mir-241 (PubMed:18794349). Despite the increase in lin-4
CC expression, post-embryonic heterochronic defects are not observed
CC (PubMed:18794349). RNAi-mediated knockdown causes precocious embryonic
CC expression of micro-RNA lin-4, exacerbated by simultaneous RNAi-
CC mediated knockdown of flh-2 (PubMed:18794349).
CC {ECO:0000269|PubMed:18794349}.
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DR EMBL; BX284604; CAA21587.2; -; Genomic_DNA.
DR EMBL; BX284604; CAO82067.1; -; Genomic_DNA.
DR EMBL; BX284604; CBK19487.1; -; Genomic_DNA.
DR PIR; T26466; T26466.
DR RefSeq; NP_001122806.1; NM_001129334.2. [Q9XWR1-2]
DR RefSeq; NP_001255383.1; NM_001268454.1. [Q9XWR1-3]
DR RefSeq; NP_501618.2; NM_069217.5. [Q9XWR1-1]
DR AlphaFoldDB; Q9XWR1; -.
DR DIP; DIP-24849N; -.
DR IntAct; Q9XWR1; 33.
DR STRING; 6239.Y11D7A.12a; -.
DR PaxDb; Q9XWR1; -.
DR EnsemblMetazoa; Y11D7A.12a.1; Y11D7A.12a.1; WBGene00012435. [Q9XWR1-1]
DR EnsemblMetazoa; Y11D7A.12b.1; Y11D7A.12b.1; WBGene00012435. [Q9XWR1-2]
DR EnsemblMetazoa; Y11D7A.12c.1; Y11D7A.12c.1; WBGene00012435. [Q9XWR1-3]
DR GeneID; 177749; -.
DR KEGG; cel:CELE_Y11D7A.12; -.
DR UCSC; Y11D7A.12b; c. elegans.
DR CTD; 177749; -.
DR WormBase; Y11D7A.12a; CE35915; WBGene00012435; flh-1.
DR WormBase; Y11D7A.12b; CE41463; WBGene00012435; flh-1.
DR WormBase; Y11D7A.12c; CE20195; WBGene00012435; flh-1.
DR eggNOG; ENOG502T0QV; Eukaryota.
DR GeneTree; ENSGT00970000196226; -.
DR HOGENOM; CLU_550096_0_0_1; -.
DR InParanoid; Q9XWR1; -.
DR OMA; SPKISVM; -.
DR OrthoDB; 1114912at2759; -.
DR PhylomeDB; Q9XWR1; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00012435; Expressed in germ line (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q9XWR1; baseline and differential.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IPI:WormBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IPI:WormBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IGI:WormBase.
DR GO; GO:0002119; P:nematode larval development; IGI:WormBase.
DR InterPro; IPR007588; Znf_FLYWCH.
DR Pfam; PF04500; FLYWCH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Metal-binding;
KW Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..507
FT /note="FLYWCH transcription factor 1"
FT /id="PRO_0000453579"
FT ZN_FING 135..192
FT /note="FLYWCH-type"
FT /evidence="ECO:0000255"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..6
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_061183"
FT VAR_SEQ 339..341
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_061184"
SQ SEQUENCE 507 AA; 56565 MW; 8E4E88625E02545A CRC64;
MYSPESMNSN ISSPSPPSSS SLNAPSLADA PEVRSDDGEA ETSEPSTSVT AVPMEIVSQA
STSAPVNQLL ASGIDMSSVI KNNAVLQALL ASASAGGFGD NAGLTMSKLL TAALANGSTA
VAKRDAGSPR TDIPKKTRLK VFSNGFFMTF DKLSSCQKKY FWRCEYKNTC KARMHTDIVT
EKILTFIHEH NHSAPIDEEV RLYGLDPTNI ERNRVYIVGN VADPNQRRKI RKQVADREAA
AKRLVQQQEE EQQKQQSASS IVAARAAYAQ AMQNGSIPTS SGVASFLQST SATAPMTSHS
MLLAQMPFLN NIKTEMSNMV KNEQFTPERY TQHMSPNLPL QTATIAPLII PTENYDSPSY
RQPAIKRKAT DLTNEEHDLR RDPMFQPTFE LARKLRKLWK GEPNRYPRTT TTPTHHFEFF
LSKNDGTDEH LYVPMRINLR DEAHLKEALQ DFCGQQCIGM LLFGISPKIS VMFNQPMLNN
WDNNQFFLLD ISNPSRWRLM YVDDQAV
