ID   FLH2_CAEEL              Reviewed;         470 AA.
AC   Q18225;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=FLYWCH transcription factor 2 {ECO:0000303|PubMed:18794349};
GN   Name=flh-2 {ECO:0000312|WormBase:C26E6.2};
GN   ORFNames=C26E6.2 {ECO:0000312|WormBase:C26E6.2};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=18794349; DOI=10.1101/gad.1678808;
RA   Ow M.C., Martinez N.J., Olsen P.H., Silverman H.S., Barrasa M.I.,
RA   Conradt B., Walhout A.J., Ambros V.;
RT   "The FLYWCH transcription factors FLH-1, FLH-2, and FLH-3 repress embryonic
RT   expression of microRNA genes in C. elegans.";
RL   Genes Dev. 22:2520-2534(2008).
CC   -!- FUNCTION: Probable transcription factor (PubMed:18794349). May bind to
CC       the promoters of target genes, including micro-RNA genes, in order to
CC       repress expression, and acting redundantly with flh-1 and flh-3
CC       (PubMed:18794349). {ECO:0000269|PubMed:18794349}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the embryo (at protein level)
CC       (PubMed:18794349). Expressed in most embryonic cells starting at
CC       gastrulation and also in head and tail cells during the larval and
CC       adult stages (PubMed:18794349). {ECO:0000269|PubMed:18794349}.
CC   -!- DISRUPTION PHENOTYPE: Knockout causes a nearly wild-type phenotype, but
CC       a few young larvae have morphological abnormalities (PubMed:18794349).
CC       In a flh-1 mutant background, L1 larvae have severe morphological
CC       abnormalities and/or appear necrotic, and 100% die before reaching the
CC       L2 larval stage (PubMed:18794349). Embryos exhibit significantly lower
CC       expression of lin-14, and overexpression of micro-RNAs, including lin-
CC       4, mir-48, mir-241, and mir-34 (PubMed:18794349). In a flh-3 mutant
CC       background, embryos exhibit significant overexpression of micro-RNAs,
CC       including lin-4, mir-48, mir-230, and mir-65 (PubMed:18794349). RNAi-
CC       mediated knockdown has no obvious phenotype, but causes precocious
CC       embryonic expression of micro-RNA lin-4, when combined with
CC       simultaneous RNAi-mediated knockdown of flh-1 (PubMed:18794349).
CC       {ECO:0000269|PubMed:18794349}.
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DR   EMBL; BX284603; CCD65726.1; -; Genomic_DNA.
DR   PIR; G88445; G88445.
DR   RefSeq; NP_498049.2; NM_065648.5.
DR   AlphaFoldDB; Q18225; -.
DR   IntAct; Q18225; 52.
DR   STRING; 6239.C26E6.2; -.
DR   EPD; Q18225; -.
DR   PaxDb; Q18225; -.
DR   PeptideAtlas; Q18225; -.
DR   EnsemblMetazoa; C26E6.2.1; C26E6.2.1; WBGene00016138.
DR   GeneID; 175670; -.
DR   KEGG; cel:CELE_C26E6.2; -.
DR   CTD; 175670; -.
DR   WormBase; C26E6.2; CE32143; WBGene00016138; flh-2.
DR   eggNOG; ENOG502TGK7; Eukaryota.
DR   GeneTree; ENSGT00970000196226; -.
DR   HOGENOM; CLU_567706_0_0_1; -.
DR   InParanoid; Q18225; -.
DR   OMA; SINRSYM; -.
DR   OrthoDB; 1114912at2759; -.
DR   PhylomeDB; Q18225; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00016138; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR   GO; GO:0005634; C:nucleus; HDA:WormBase.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IGI:WormBase.
DR   GO; GO:0002119; P:nematode larval development; IGI:WormBase.
DR   InterPro; IPR007588; Znf_FLYWCH.
DR   Pfam; PF04500; FLYWCH; 1.
PE   1: Evidence at protein level;
KW   Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT   CHAIN           1..470
FT                   /note="FLYWCH transcription factor 2"
FT                   /id="PRO_0000453578"
FT   ZN_FING         145..204
FT                   /note="FLYWCH-type"
FT                   /evidence="ECO:0000255"
FT   REGION          102..148
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        102..143
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   470 AA;  52068 MW;  FD3EB53080521712 CRC64;
     MVDTMMNTNT DGVTAVPPIF PLPSTTPNRN NNNELAMLAQ KQIVAGLLLQ QPTIVQAAVN
     GTNSQKHLAD IDYTNVIQTL QALGTTNLKA FSLLLDPQSQ TSQLISEDTR PSASSSPSST
     ATAVSNSGQS NATSTSSSST EPEYKPRNVR EKVYADGYIM SFDKKSCCGT KEFWRCERKN
     DCNARMHSDI NTREIVRKLH PHNHEKPSPE ELAFYEQDFS TLHPNYCHPV KSINRSYMQR
     KLSRASHVSQ ALPIAPQQIK AEPMEVDTTN QTIQNFQQNN NLMLLASINA AATAAAAATS
     AGISPPVVGQ KRVSTTVMPI TLKSQRTTPK EEEDSSQLMS QEEFRITYEL TKKLMEMMKP
     KTEIGVRWQG DEGSILLFLS HDNGAEENVF FPVVVMNRDE KSLITALEGF TGKRCEGKLA
     LCYSQRVNVL VHEALISNWT HGKLFLVNTD APALWRLTPV DAFGEPLNHV