FLHA_BACSU
ID FLHA_BACSU Reviewed; 677 AA.
AC P35620;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Flagellar biosynthesis protein FlhA;
GN Name=flhA; OrderedLocusNames=BSU16390;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8097015; DOI=10.1111/j.1365-2958.1993.tb01164.x;
RA Carpenter P.B., Ordal G.W.;
RT "Bacillus subtilis FlhA: a flagellar protein related to a new family of
RT signal-transducing receptors.";
RL Mol. Microbiol. 7:735-743(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 18-19.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
CC -!- FUNCTION: Involved in the export of flagellum proteins.
CC -!- INTERACTION:
CC P35620; P20487: fliJ; NbExp=3; IntAct=EBI-15858509, EBI-15858572;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the FHIPEP (flagella/HR/invasion proteins export
CC pore) family. {ECO:0000305}.
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DR EMBL; X63698; CAA45225.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13512.2; -; Genomic_DNA.
DR PIR; S33664; S33664.
DR RefSeq; NP_389521.2; NC_000964.3.
DR RefSeq; WP_003231947.1; NZ_JNCM01000035.1.
DR PDB; 3MIX; X-ray; 2.30 A; A=303-677.
DR PDBsum; 3MIX; -.
DR AlphaFoldDB; P35620; -.
DR SMR; P35620; -.
DR DIP; DIP-59539N; -.
DR IntAct; P35620; 5.
DR STRING; 224308.BSU16390; -.
DR PaxDb; P35620; -.
DR PRIDE; P35620; -.
DR EnsemblBacteria; CAB13512; CAB13512; BSU_16390.
DR GeneID; 940029; -.
DR KEGG; bsu:BSU16390; -.
DR PATRIC; fig|224308.179.peg.1780; -.
DR eggNOG; COG1298; Bacteria.
DR InParanoid; P35620; -.
DR OMA; MAINPGQ; -.
DR PhylomeDB; P35620; -.
DR BioCyc; BSUB:BSU16390-MON; -.
DR EvolutionaryTrace; P35620; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0044780; P:bacterial-type flagellum assembly; IMP:CACAO.
DR GO; GO:0071978; P:bacterial-type flagellum-dependent swarming motility; IMP:CACAO.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 1.10.8.540; -; 1.
DR Gene3D; 3.40.30.60; -; 1.
DR Gene3D; 3.40.50.12790; -; 1.
DR InterPro; IPR042194; FHIPEP_1.
DR InterPro; IPR042193; FHIPEP_3.
DR InterPro; IPR042196; FHIPEP_4.
DR InterPro; IPR025505; FHIPEP_CS.
DR InterPro; IPR006301; FlhA.
DR InterPro; IPR001712; T3SS_FHIPEP.
DR PANTHER; PTHR30161; PTHR30161; 1.
DR Pfam; PF00771; FHIPEP; 1.
DR PIRSF; PIRSF005419; FlhA; 1.
DR PRINTS; PR00949; TYPE3IMAPROT.
DR TIGRFAMs; TIGR01398; FlhA; 1.
DR PROSITE; PS00994; FHIPEP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacterial flagellum biogenesis;
KW Bacterial flagellum protein export; Cell membrane; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..677
FT /note="Flagellar biosynthesis protein FlhA"
FT /id="PRO_0000190010"
FT TRANSMEM 6..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 18..19
FT /note="ML -> IV (in Ref. 1; CAA45225)"
FT /evidence="ECO:0000305"
FT HELIX 319..326
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 332..335
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 336..338
FT /evidence="ECO:0007829|PDB:3MIX"
FT STRAND 343..348
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 350..352
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 353..357
FT /evidence="ECO:0007829|PDB:3MIX"
FT STRAND 358..360
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 364..379
FT /evidence="ECO:0007829|PDB:3MIX"
FT STRAND 387..390
FT /evidence="ECO:0007829|PDB:3MIX"
FT STRAND 398..403
FT /evidence="ECO:0007829|PDB:3MIX"
FT STRAND 406..412
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 465..484
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 487..500
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 502..508
FT /evidence="ECO:0007829|PDB:3MIX"
FT TURN 509..512
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 515..527
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 535..545
FT /evidence="ECO:0007829|PDB:3MIX"
FT TURN 546..548
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 552..562
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 564..571
FT /evidence="ECO:0007829|PDB:3MIX"
FT STRAND 581..583
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 585..594
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 608..627
FT /evidence="ECO:0007829|PDB:3MIX"
FT STRAND 634..636
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 638..648
FT /evidence="ECO:0007829|PDB:3MIX"
FT TURN 649..651
FT /evidence="ECO:0007829|PDB:3MIX"
FT STRAND 657..659
FT /evidence="ECO:0007829|PDB:3MIX"
FT HELIX 660..662
FT /evidence="ECO:0007829|PDB:3MIX"
SQ SEQUENCE 677 AA; 73977 MW; C7FF66FA886D7203 CRC64;
MSTRDLSVLI SVVLIVAMLV IPFPPWLLSI LIIINISLAL IVLLTTMNMQ EALQFSIFPS
LLLLLTLFRL GLNVSTTRSI LSHGEGGKVV ETFGNFVVGG NVLVGLVVFI ILIIIQFIVI
TKGAERVSEV AARFTLDAMP GKQMSIDADL NAGMITEQEA KHRREKVARE ADFYGAMDGA
SKFVKGDAIA GIIIVMINII FGIVIGMLQQ GMSIQEAASH FTMLTVGDGI VSQIPALLIS
TATGIVVTRA ASEGNLGHDI TGQLFAYPKL LYVAAATIML LGIFTPIGIL LTGPLAGLLA
FGAYTLSKSG KEKEEVDEIL EEEAEVDELK SPESVVQLLH IDPIEFEFGY GLIPLADANQ
GGDLLDRIVM IRRQLALELG LVIPVVRIRD NIALQPNEYR LKIKGNEVAK GELLLDHYLA
MSPTPEDDLI EGIETVEPSF GLPAKWISEA VKDEADMLGY TVVDPASVVS THITEKIKQH
AHELIGRQET KQLIDHLKES YPVLVEEVTP NPLSVGDIQK VLAKLLKEKV SIRNLVTIFE
TLADYGKLTT DSDLLTEYTR QALAKQITAQ FAKENEVLKV VTCSGRVEKA IADGVQQTEH
GNYLSLEPDI SESIVRSVAK EAEQLSLRQE TAILLCSPPV RMYVKQLLER YFPDLPVLSY
NELEANVEVQ SIGVVDI
