FLHA_BRUME
ID FLHA_BRUME Reviewed; 723 AA.
AC Q8YDK9; Q8YDK8;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Flagellar biosynthesis protein FlhA;
GN Name=flhA; OrderedLocusNames=BMEII0166/BMEII0167;
OS Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=224914;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=11756688; DOI=10.1073/pnas.221575398;
RA DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA Haselkorn R., Kyrpides N.C., Overbeek R.;
RT "The genome sequence of the facultative intracellular pathogen Brucella
RT melitensis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
RN [2]
RP FUNCTION IN INFECTION PERSISTENCE.
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=15839898; DOI=10.1111/j.1462-5822.2005.00502.x;
RA Fretin D., Fauconnier A., Koehler S., Halling S., Leonard S., Nijskens C.,
RA Ferooz J., Lestrate P., Delrue R.-M., Danese I., Vandenhaute J., Tibor A.,
RA DeBolle X., Letesson J.-J.;
RT "The sheathed flagellum of Brucella melitensis is involved in persistence
RT in a murine model of infection.";
RL Cell. Microbiol. 7:687-698(2005).
CC -!- FUNCTION: Required for formation of the rod structure of the flagellar
CC apparatus. May constitute the export apparatus of flagellin (By
CC similarity). The flagellum is required to cause a persistent disease in
CC a murine model of infection. {ECO:0000250,
CC ECO:0000269|PubMed:15839898}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the FHIPEP (flagella/HR/invasion proteins export
CC pore) family. {ECO:0000305}.
CC -!- CAUTION: Despite the presence of a stop codon in position 243 in the
CC fliF gene and in position 127 in the flhA gene, it has been shown that
CC B.melitensis is able to express genes corresponding to the M ring, the
CC hook and the filament of the flagellar apparatus in the early log phase
CC of growth in 2YT broth. Under these conditions, a polar and sheathed
CC flagellar structure is visible by transmission electron microscopy.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL53407.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE008918; AAL53407.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE008918; AAL53408.1; ALT_SEQ; Genomic_DNA.
DR PIR; AD3530; AD3530.
DR PIR; AE3530; AE3530.
DR AlphaFoldDB; Q8YDK9; -.
DR SMR; Q8YDK9; -.
DR STRING; 224914.BMEII0167; -.
DR EnsemblBacteria; AAL53407; AAL53407; BMEII0166.
DR EnsemblBacteria; AAL53408; AAL53408; BMEII0167.
DR KEGG; bme:BMEII0166; -.
DR KEGG; bme:BMEII0167; -.
DR eggNOG; COG1298; Bacteria.
DR PRO; PR:Q8YDK9; -.
DR Proteomes; UP000000419; Chromosome II.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044780; P:bacterial-type flagellum assembly; IEA:InterPro.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 1.10.8.540; -; 1.
DR Gene3D; 3.40.30.60; -; 1.
DR Gene3D; 3.40.50.12790; -; 1.
DR InterPro; IPR042194; FHIPEP_1.
DR InterPro; IPR042193; FHIPEP_3.
DR InterPro; IPR042196; FHIPEP_4.
DR InterPro; IPR006301; FlhA.
DR InterPro; IPR001712; T3SS_FHIPEP.
DR PANTHER; PTHR30161; PTHR30161; 1.
DR Pfam; PF00771; FHIPEP; 1.
DR PIRSF; PIRSF005419; FlhA; 1.
DR PRINTS; PR00949; TYPE3IMAPROT.
DR TIGRFAMs; TIGR01398; FlhA; 1.
PE 1: Evidence at protein level;
KW Bacterial flagellum biogenesis; Bacterial flagellum protein export;
KW Cell membrane; Membrane; Protein transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..723
FT /note="Flagellar biosynthesis protein FlhA"
FT /id="PRO_0000323999"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 361..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 723 AA; 78724 MW; 97D990DA94BD7160 CRC64;
MKQTLGAVPA ARLTGTGPMS VAKQEADVQQ AAAKPFIKGK FLTGSDIGLA VGIIIILTVL
FLPVPAVVLD IGLAFSIAFS VLILMVALWI QRPLDFSAFP TVLLIATMMR LSLNIATTRV
ILTHGNEGYL AAGHVIHGFS QFVMGGDFVI GLVVFAILII VNFLVITKGA TRIAEVGARF
TLDAIPGKQM AIDADLSSGL IDEKEAQRRR RELEEESSFF GSMDGASKFV RGDAIAGLII
TAVNIFGGIV IGATRHGMDI SQAADVFTKL SVGDGLVTQI PALIVSLAAG LLVSKGGTRG
SADQAIFGQL GAYPKALLIA ALLLFILGVM PGLPAFPFFL LGGAMAFVGI AVPRRQARQR
EADAAEAGKK QREAEEQERN SVKASLETNQ IELCLGKQLS ARLIASQEEL AHRVNKMRRK
FAQEYGFVIP EIKVTDDIAL PPKSYRIKIH GTAVASHELR VGEILVVLGE RPVPSVPGEE
VREPAFGMRA YSVPETFTAD LRREGYMTVD NLSVLLTHLS EIVRNNLAQL LSYKDMRILL
DRLGPEYRKL LEDICPAHIS YSGLQAVLKL LLAERISIRN LHLILEAIAE IAPLVRRPEM
IVEHVRMRMA QQICGDLSDN GVLNVLRLGN RWDLVFHQSL KRDAKGEIVE FDIDPRLLEQ
FGTEASAAIR KHFDNGERFV LVSSPEARPY IRMIIERLFA TLPVLSHVEI ARGVEVKSLG
AIS
