FLHA_ECOLI
ID FLHA_ECOLI Reviewed; 692 AA.
AC P76298; O87956;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Flagellar biosynthesis protein FlhA;
GN Name=flhA; OrderedLocusNames=b1879, JW1868;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Cho M., Matsumura P.;
RT "Genomic organization of flhB operon in E.coli.";
RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Required for formation of the rod structure of the flagellar
CC apparatus. Together with FliI and FliH, may constitute the export
CC apparatus of flagellin.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the FHIPEP (flagella/HR/invasion proteins export
CC pore) family. {ECO:0000305}.
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DR EMBL; U88319; AAC17835.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74949.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15688.1; -; Genomic_DNA.
DR PIR; G64950; G64950.
DR RefSeq; NP_416393.1; NC_000913.3.
DR RefSeq; WP_000066951.1; NZ_LN832404.1.
DR AlphaFoldDB; P76298; -.
DR SMR; P76298; -.
DR BioGRID; 4260377; 26.
DR ComplexPortal; CPX-5885; Flagellar export complex.
DR IntAct; P76298; 1.
DR STRING; 511145.b1879; -.
DR TCDB; 1.A.104.1.1; the proposed flagellar biosynthesis na+ channel, flha (flha) family.
DR PaxDb; P76298; -.
DR PRIDE; P76298; -.
DR EnsemblBacteria; AAC74949; AAC74949; b1879.
DR EnsemblBacteria; BAA15688; BAA15688; BAA15688.
DR GeneID; 946390; -.
DR KEGG; ecj:JW1868; -.
DR KEGG; eco:b1879; -.
DR PATRIC; fig|511145.12.peg.1959; -.
DR EchoBASE; EB4021; -.
DR eggNOG; COG1298; Bacteria.
DR InParanoid; P76298; -.
DR OMA; MAINPGQ; -.
DR PhylomeDB; P76298; -.
DR BioCyc; EcoCyc:G370-MON; -.
DR PRO; PR:P76298; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009288; C:bacterial-type flagellum; IC:ComplexPortal.
DR GO; GO:0120102; C:bacterial-type flagellum secretion apparatus; IC:ComplexPortal.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0030257; C:type III protein secretion system complex; IC:ComplexPortal.
DR GO; GO:0044780; P:bacterial-type flagellum assembly; IMP:EcoCyc.
DR GO; GO:0071973; P:bacterial-type flagellum-dependent cell motility; IC:ComplexPortal.
DR GO; GO:0006935; P:chemotaxis; IC:ComplexPortal.
DR GO; GO:0030254; P:protein secretion by the type III secretion system; IC:ComplexPortal.
DR Gene3D; 1.10.8.540; -; 1.
DR Gene3D; 3.40.30.60; -; 1.
DR Gene3D; 3.40.50.12790; -; 1.
DR InterPro; IPR042194; FHIPEP_1.
DR InterPro; IPR042193; FHIPEP_3.
DR InterPro; IPR042196; FHIPEP_4.
DR InterPro; IPR025505; FHIPEP_CS.
DR InterPro; IPR006301; FlhA.
DR InterPro; IPR001712; T3SS_FHIPEP.
DR PANTHER; PTHR30161; PTHR30161; 1.
DR Pfam; PF00771; FHIPEP; 1.
DR PIRSF; PIRSF005419; FlhA; 1.
DR PRINTS; PR00949; TYPE3IMAPROT.
DR TIGRFAMs; TIGR01398; FlhA; 1.
DR PROSITE; PS00994; FHIPEP; 1.
PE 3: Inferred from homology;
KW Bacterial flagellum biogenesis; Bacterial flagellum protein export;
KW Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..692
FT /note="Flagellar biosynthesis protein FlhA"
FT /id="PRO_0000190016"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 328
FT /note="R -> S (in Ref. 1; AAC17835)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 692 AA; 74843 MW; 558B235085DE69C5 CRC64;
MSNLAAMLRL PANLKSTQWQ ILAGPILILL ILSMMVLPLP AFILDLLFTF NIALSIMVLL
VAMFTQRTLE FAAFPTILLF TTLLRLALNV ASTRIILMEG HTGAAAAGKV VEAFGHFLVG
GNFAIGIVVF VILVIINFMV ITKGAGRIAE VGARFVLDGM PGKQMAIDAD LNAGLIGEDE
AKKRRSEVTQ EADFYGSMDG ASKFVRGDAI AGILIMVINI VGGLLVGVLQ HGMSMGHAAE
SYTLLTIGDG LVAQIPALVI STAAGVIVTR VSTDQDVGEQ MVNQLFSNPS VMLLSAAVLG
LLGLVPGMPN LVFLLFTAGL LGLAWWIRGR EQKAPAEPKP VKMAENNTVV EATWNDVQLE
DSLGMEVGYR LIPMVDFQQD GELLGRIRSI RKKFAQEMGF LPPVVHIRDN MDLQPARYRI
LMKGVEIGSG DAYPGRWLAI NPGTAAGTLP GEATVDPAFG LNAIWIESAL KEQAQIQGYT
VVEASTVVAT HLNHLISQHA AELFGRQEAQ QLLDRVAQEM PKLTEDLVPG VVTLTTLHKV
LQNLLDEKVP IRDMRTILET LAEHAPIQSD PHELTAVVRV ALGRAITQQW FPGKDEVHVI
GLDTPLERLL LQALQGGGGL EPGLADRLLA QTQEALSRQE MLGAPPVLLV NHALRPLLSR
FLRRSLPQLV VLSNLELSDN RHIRMTATIG GK
