ID   FLHB_ECOLI              Reviewed;         382 AA.
AC   P76299; O87955;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Flagellar biosynthetic protein FlhB;
GN   Name=flhB; Synonyms=yecQ; OrderedLocusNames=b1880, JW1869;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Cho M., Matsumura P.;
RT   "Genomic organization of flhB operon in E.coli.";
RL   Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Required for formation of the rod structure in the basal body
CC       of the flagellar apparatus. Together with FliI and FliH, may constitute
CC       the export apparatus of flagellin.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the type III secretion exporter family.
CC       {ECO:0000305}.
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DR   EMBL; U88319; AAC17834.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74950.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15696.1; -; Genomic_DNA.
DR   PIR; H64950; H64950.
DR   RefSeq; NP_416394.1; NC_000913.3.
DR   RefSeq; WP_001278954.1; NZ_STEB01000026.1.
DR   AlphaFoldDB; P76299; -.
DR   SMR; P76299; -.
DR   BioGRID; 4260378; 21.
DR   ComplexPortal; CPX-5885; Flagellar export complex.
DR   IntAct; P76299; 3.
DR   STRING; 511145.b1880; -.
DR   MEROPS; N06.A01; -.
DR   PaxDb; P76299; -.
DR   PRIDE; P76299; -.
DR   EnsemblBacteria; AAC74950; AAC74950; b1880.
DR   EnsemblBacteria; BAA15696; BAA15696; BAA15696.
DR   GeneID; 66674229; -.
DR   GeneID; 946391; -.
DR   KEGG; ecj:JW1869; -.
DR   KEGG; eco:b1880; -.
DR   PATRIC; fig|1411691.4.peg.368; -.
DR   EchoBASE; EB3789; -.
DR   eggNOG; COG1377; Bacteria.
DR   HOGENOM; CLU_041013_1_2_6; -.
DR   InParanoid; P76299; -.
DR   OMA; DEYKQQE; -.
DR   PhylomeDB; P76299; -.
DR   BioCyc; EcoCyc:G7028-MON; -.
DR   PRO; PR:P76299; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009288; C:bacterial-type flagellum; IC:ComplexPortal.
DR   GO; GO:0120102; C:bacterial-type flagellum secretion apparatus; IC:ComplexPortal.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0030257; C:type III protein secretion system complex; IC:ComplexPortal.
DR   GO; GO:0044780; P:bacterial-type flagellum assembly; IEA:InterPro.
DR   GO; GO:0071973; P:bacterial-type flagellum-dependent cell motility; IC:ComplexPortal.
DR   GO; GO:0006935; P:chemotaxis; IC:ComplexPortal.
DR   GO; GO:0030254; P:protein secretion by the type III secretion system; IC:ComplexPortal.
DR   Gene3D; 3.40.1690.10; -; 1.
DR   InterPro; IPR006136; FlhB.
DR   InterPro; IPR006135; T3SS_substrate_exporter.
DR   InterPro; IPR029025; T3SS_substrate_exporter_C.
DR   PANTHER; PTHR30531; PTHR30531; 1.
DR   Pfam; PF01312; Bac_export_2; 1.
DR   PRINTS; PR00950; TYPE3IMSPROT.
DR   SUPFAM; SSF160544; SSF160544; 1.
DR   TIGRFAMs; TIGR00328; flhB; 1.
PE   1: Evidence at protein level;
KW   Bacterial flagellum biogenesis; Bacterial flagellum protein export;
KW   Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..382
FT                   /note="Flagellar biosynthetic protein FlhB"
FT                   /id="PRO_0000180949"
FT   TOPO_DOM        1..31
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        53..73
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        74..94
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        116..144
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        166..189
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        190..210
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        211..382
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        250
FT                   /note="A -> R (in Ref. 1; AAC17834)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        316
FT                   /note="P -> R (in Ref. 1; AAC17834)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   382 AA;  42238 MW;  3E319DFC30FEC4CC CRC64;
     MSDESDDKTE APTPHRLEKA REEGQIPRSR ELTSLLILLV GVSVIWFGGV SLARRLSGML
     SAGLHFDHSI INDPNLILGQ IILLIREAML ALLPLISGVV LVALISPVML GGLVFSGKSL
     QPKFSKLNPL PGIKRMFSAQ TGAELLKAIL KTILVGSVTG FFLWHHWPQM MRLMAESPIT
     AMGNAMDLVG LCALLVVLGV IPMVGFDVFF QIFSHLKKLR MSRQDIRDEF KQSEGDPHVK
     GRIRQMQRAA ARRRMMADVP KADVIVNNPT HYSVALQYDE NKMSAPKVVA KGAGLVALRI
     REIGAENNVP TLEAPPLARA LYRHAEIGQQ IPGQLYAAVA EVLAWVWQLK RWRLAGGQRP
     VQPTHLPVPE ALDFINEKPT HE