ID   AKTP1_DROME             Reviewed;         244 AA.
AC   Q7K4V4; Q7JRK3;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Protein crossbronx;
GN   ORFNames=CG46338 {ECO:0000312|FlyBase:FBgn0285962};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
RC   STRAIN=Berkeley; TISSUE=Embryo, and Head;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=B;
CC         IsoId=Q7K4V4-1; Sequence=Displayed;
CC       Name=A;
CC         IsoId=Q7K4V4-2; Sequence=VSP_037634;
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. FTS
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- CAUTION: Lacks the conserved Cys residue necessary for ubiquitin-
CC       conjugating enzyme E2 activity. {ECO:0000305}.
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DR   EMBL; AE013599; AAF58884.1; -; Genomic_DNA.
DR   EMBL; AE013599; AAG22290.1; -; Genomic_DNA.
DR   EMBL; AY051650; AAK93074.1; -; mRNA.
DR   EMBL; BT001497; AAN71252.1; -; mRNA.
DR   RefSeq; NP_524888.1; NM_080149.4. [Q7K4V4-1]
DR   RefSeq; NP_724848.1; NM_165716.2. [Q7K4V4-2]
DR   AlphaFoldDB; Q7K4V4; -.
DR   SMR; Q7K4V4; -.
DR   BioGRID; 70725; 2.
DR   STRING; 7227.FBpp0087538; -.
DR   PaxDb; Q7K4V4; -.
DR   DNASU; 47272; -.
DR   EnsemblMetazoa; FBtr0472692; FBpp0422655; FBgn0285962. [Q7K4V4-2]
DR   EnsemblMetazoa; FBtr0472693; FBpp0422656; FBgn0285962. [Q7K4V4-1]
DR   GeneID; 47272; -.
DR   KEGG; dme:Dmel_CG46338; -.
DR   UCSC; CG10536-RA; d. melanogaster.
DR   UCSC; CG10536-RB; d. melanogaster. [Q7K4V4-1]
DR   FlyBase; FBgn0285962; CG46338.
DR   VEuPathDB; VectorBase:FBgn0285962; -.
DR   eggNOG; KOG0429; Eukaryota.
DR   GeneTree; ENSGT00390000010125; -.
DR   InParanoid; Q7K4V4; -.
DR   OMA; QVLKYMQ; -.
DR   PhylomeDB; Q7K4V4; -.
DR   BioGRID-ORCS; 47272; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 47272; -.
DR   PRO; PR:Q7K4V4; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0285962; Expressed in mouthpart and 25 other tissues.
DR   ExpressionAtlas; Q7K4V4; baseline and differential.
DR   Genevisible; Q7K4V4; DM.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:FlyBase.
DR   GO; GO:0042742; P:defense response to bacterium; IMP:FlyBase.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:FlyBase.
DR   GO; GO:0007291; P:sperm individualization; IMP:FlyBase.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Reference proteome.
FT   CHAIN           1..244
FT                   /note="Protein crossbronx"
FT                   /id="PRO_0000379032"
FT   DOMAIN          20..176
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   VAR_SEQ         1..30
FT                   /note="Missing (in isoform A)"
FT                   /evidence="ECO:0000303|PubMed:12537569"
FT                   /id="VSP_037634"
SQ   SEQUENCE   244 AA;  28133 MW;  FC51CC219063BC8A CRC64;
     MTLDLDAHKK DDKLLITTIQ QEYKILAEYK MIESEKLSGI YVIPSYANSL QWFGVFFGRQ
     GLYAESVFRF TILLPDRFPD DKSLPSIIFQ QDVIHPHVCP YTHSLDVSHA FPEWRCGEDH
     LWQLLKYLQV IFSDPLDSIR GIEVDKLKNS EAAELLMNNK EEYVARVQEN IKESKEHIFD
     TPPTEDPHYI VFEKFQQDVH GPVLERIKAG RSKLTEPSAQ QANGGHATGL SWVKEGEFKP
     LSIE