ID   FLHF_AQUAE              Reviewed;         373 AA.
AC   O67266;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Flagellar biosynthesis protein FlhF;
DE   AltName: Full=Flagella-associated GTP-binding protein;
GN   Name=flhF; OrderedLocusNames=aq_1214;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- FUNCTION: Necessary for flagellar biosynthesis. May be involved in
CC       translocation of the flagellum (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. {ECO:0000305}.
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DR   EMBL; AE000657; AAC07228.1; -; Genomic_DNA.
DR   PIR; G70404; G70404.
DR   RefSeq; NP_213830.1; NC_000918.1.
DR   RefSeq; WP_010880768.1; NC_000918.1.
DR   AlphaFoldDB; O67266; -.
DR   SMR; O67266; -.
DR   STRING; 224324.aq_1214; -.
DR   EnsemblBacteria; AAC07228; AAC07228; aq_1214.
DR   KEGG; aae:aq_1214; -.
DR   PATRIC; fig|224324.8.peg.944; -.
DR   eggNOG; COG1419; Bacteria.
DR   HOGENOM; CLU_009301_11_4_0; -.
DR   InParanoid; O67266; -.
DR   OMA; WYEVDEY; -.
DR   OrthoDB; 1162441at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR   GO; GO:0005047; F:signal recognition particle binding; IBA:GO_Central.
DR   GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR   GO; GO:0006605; P:protein targeting; IBA:GO_Central.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR020006; FlhF.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   PANTHER; PTHR43134:SF3; PTHR43134:SF3; 2.
DR   Pfam; PF00448; SRP54; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum biogenesis; Bacterial flagellum protein export;
KW   Cell membrane; GTP-binding; Membrane; Nucleotide-binding;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..373
FT                   /note="Flagellar biosynthesis protein FlhF"
FT                   /id="PRO_0000101219"
FT   BINDING         179..186
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         255..259
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         312..315
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   373 AA;  42827 MW;  478520EB635E8F32 CRC64;
     MKIEKIEVNS LQEAIEIVER RFGENAVILS SRVIRKNRFG FLPIFRKKIL EVTVGIREEE
     DFRKEFERER ELIKEIENLK KIVNELINNG EVKGEKIVEK GSDTYAPRVK RYLEKLVIKN
     ISKNIAEKII QDACGYDIDN KIYDFKDEPY TSLRESIEKN IKLNEEFIQN PPKVVALVGP
     TGVGKTTTLA KLAHLFKKNK KKVGIISLDC FRVGAFEQLK AFAEVLEVPF KLADSPRAFN
     IQLLEMDDRD VILVDTAGRS HYDVIRLKEL ETYFKVSDIS VYLTLAANLS ELVMYEAIMQ
     FGMFSISGLI FTKLDETPYP GSMVNVAYRT QYPVVCFTMG QSIPEDIVVA NYDYLVRLIL
     EDEDEVRPAT QLA