ID   FLHF_BORBU              Reviewed;         388 AA.
AC   Q44758; Q44910;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Flagellar biosynthesis protein FlhF;
DE   AltName: Full=Flagella-associated GTP-binding protein;
GN   Name=flhF; OrderedLocusNames=BB_0270;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RA   Dunn J.J., Butler-Loffredo L., Kieleczawa J., Medalle J., Luft B.J.;
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=212;
RX   PubMed=9168127; DOI=10.1016/s0378-1119(96)00848-7;
RA   Ge Y., Charon N.W.;
RT   "Identification of a large motility operon in Borrelia burgdorferi by semi-
RT   random PCR chromosome walking.";
RL   Gene 189:195-201(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
CC   -!- FUNCTION: Necessary for flagellar biosynthesis. May be involved in
CC       translocation of the flagellum (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. {ECO:0000305}.
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DR   EMBL; U43739; AAA85594.1; -; Genomic_DNA.
DR   EMBL; L75945; AAB58973.1; -; Genomic_DNA.
DR   EMBL; AE000783; AAC66678.1; -; Genomic_DNA.
DR   PIR; F70133; F70133.
DR   RefSeq; NP_212404.1; NC_001318.1.
DR   RefSeq; WP_002556869.1; NC_001318.1.
DR   AlphaFoldDB; Q44758; -.
DR   SMR; Q44758; -.
DR   STRING; 224326.BB_0270; -.
DR   PRIDE; Q44758; -.
DR   EnsemblBacteria; AAC66678; AAC66678; BB_0270.
DR   GeneID; 56567701; -.
DR   KEGG; bbu:BB_0270; -.
DR   PATRIC; fig|224326.49.peg.669; -.
DR   HOGENOM; CLU_009301_11_4_12; -.
DR   OMA; WYEVDEY; -.
DR   Proteomes; UP000001807; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:CAFA.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR020006; FlhF.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   PANTHER; PTHR43134:SF3; PTHR43134:SF3; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR03499; FlhF; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum biogenesis; Bacterial flagellum protein export;
KW   Cell membrane; GTP-binding; Membrane; Nucleotide-binding;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..388
FT                   /note="Flagellar biosynthesis protein FlhF"
FT                   /id="PRO_0000101221"
FT   BINDING         181..188
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         261..265
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         320..323
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        39
FT                   /note="Missing (in Ref. 2; AAB58973)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        380
FT                   /note="K -> R (in Ref. 2; AAB58973)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   388 AA;  44369 MW;  82AC925B84E2BC11 CRC64;
     MVQYFTEKGP TYNEVIEIIK KKYGKNARVM TYKTVPHGGI LGLFSKDWVE VSGYVRYDIG
     NQQINVEDEK RKILQSIKRE ENSSIEDVLK EVKSLKTELA HKKENINHPT ITKIEDILRE
     NDFSENYIKD INEFIKREFS LSDLDDYERV REDVVLYIAK TIKCSGSIID NLKKRVFILV
     GPTGVGKTTT IAKLAAIYGI NGESKSLNIK IITIDNYRIG AKKQIQTYGD IMGIPVRAIE
     SFKDLKDEIT DSKDFDLILV DTIGKSPKDF MKLAEMKELL NACGRDAEFH LAVSSTTKTS
     DVKEIFHQFS PFNYKTVIFT KVDETTCVGN LISLIYEMKK VVSYVTDGQI VPHNISVAEP
     LTFIRRINGY RISDDAEFIK KIKSKSYY