ID   FLHF_CAMJE              Reviewed;         484 AA.
AC   O52908; Q0PC69; Q9PJ55;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Flagellar biosynthesis protein FlhF;
DE   AltName: Full=Flagella-associated GTP-binding protein;
GN   Name=flhF; OrderedLocusNames=Cj0064c;
OS   Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC
OS   11168).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=192222;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700819 / NCTC 11168;
RX   PubMed=10688204; DOI=10.1038/35001088;
RA   Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA   Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA   Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A.,
RA   Rajandream M.A., Rutherford K.M., van Vliet A.H.M., Whitehead S.,
RA   Barrell B.G.;
RT   "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT   reveals hypervariable sequences.";
RL   Nature 403:665-668(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 374-484.
RC   STRAIN=ATCC 700819 / NCTC 11168;
RA   Karlyshev A.V., Wren B.W.;
RT   "Cloning and characterisation of flhF gene of C. jejuni.";
RL   Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Necessary for flagellar biosynthesis. May be involved in
CC       translocation of the flagellum (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. {ECO:0000305}.
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DR   EMBL; AL111168; CAL34238.1; -; Genomic_DNA.
DR   EMBL; AJ000857; CAA04334.1; -; Genomic_DNA.
DR   PIR; D81422; D81422.
DR   RefSeq; WP_002851941.1; NC_002163.1.
DR   RefSeq; YP_002343528.1; NC_002163.1.
DR   AlphaFoldDB; O52908; -.
DR   SMR; O52908; -.
DR   IntAct; O52908; 5.
DR   STRING; 192222.Cj0064c; -.
DR   PaxDb; O52908; -.
DR   PRIDE; O52908; -.
DR   EnsemblBacteria; CAL34238; CAL34238; Cj0064c.
DR   GeneID; 904464; -.
DR   KEGG; cje:Cj0064c; -.
DR   PATRIC; fig|192222.6.peg.63; -.
DR   eggNOG; COG1419; Bacteria.
DR   HOGENOM; CLU_009301_11_3_7; -.
DR   OMA; LIQNMFW; -.
DR   PHI-base; PHI:8627; -.
DR   Proteomes; UP000000799; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR020006; FlhF.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   PANTHER; PTHR43134:SF3; PTHR43134:SF3; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR03499; FlhF; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum biogenesis; Bacterial flagellum protein export;
KW   Cell membrane; GTP-binding; Membrane; Nucleotide-binding;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..484
FT                   /note="Flagellar biosynthesis protein FlhF"
FT                   /id="PRO_0000101222"
FT   REGION          64..91
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        72..88
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         289..296
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         367..371
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         426..429
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        374
FT                   /note="Y -> T (in Ref. 2; CAA04334)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        377
FT                   /note="S -> I (in Ref. 2; CAA04334)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        472..484
FT                   /note="CILEGFNKGKNNE -> WYFRRV (in Ref. 2; CAA04334)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   484 AA;  55356 MW;  50BB174F3BEBB357 CRC64;
     MGQLIHTFTV EDTEQIIPKV KEDYGDKALI ITNKQIRPKT LNRSALYEVM VAIEESDYEE
     HLKKQGKSLP AKKSSPKPSS TSLAEEKIRS QIPQEDEDVV LDFSNTRLNT NLNTVKNNDL
     AKKTYQDFPQ NKINPHQNKT LGFDDFKEKL SEVSNEISKV TNTPLENYTP NPNYNKKIEN
     FEKQFEKQIN KLNDKIDLLA DMMWDDKAEA RKNLMIPPEF ASIYKQAKES GMLENHLEAI
     MKATIENMPA AMKTNKDAVQ RYFHSLLRNI LPCRVESDIK KQKIMMLVGP TGVGKTTTLA
     KLAFRYAYGD KRYKTGIITL DTYRIGAVEQ LFQYAKMMKL PIIDSIEPKD LDEAIKSLNN
     CEVILVDTIG NSQYDQSKLA KTKEFLMHSN AEIDVNLVVS ANTKHEDLME IYKNFSFLNI
     DTLIITKFDE TKVFGNIFSL VYETNIPLSF FSIGQEVPDD LEVANSDFLV HCILEGFNKG
     KNNE