ID   FLHF_PSEAE              Reviewed;         429 AA.
AC   Q9I3P8;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Flagellar biosynthesis protein FlhF;
DE   AltName: Full=Flagella-associated GTP-binding protein;
GN   Name=flhF; OrderedLocusNames=PA1453;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Necessary for flagellar biosynthesis. May be involved in
CC       translocation of the flagellum (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG04842.1; -; Genomic_DNA.
DR   PIR; A83463; A83463.
DR   RefSeq; NP_250144.1; NC_002516.2.
DR   RefSeq; WP_003114281.1; NZ_QZGE01000005.1.
DR   AlphaFoldDB; Q9I3P8; -.
DR   SMR; Q9I3P8; -.
DR   STRING; 287.DR97_570; -.
DR   PaxDb; Q9I3P8; -.
DR   PRIDE; Q9I3P8; -.
DR   EnsemblBacteria; AAG04842; AAG04842; PA1453.
DR   GeneID; 881865; -.
DR   KEGG; pae:PA1453; -.
DR   PATRIC; fig|208964.12.peg.1504; -.
DR   PseudoCAP; PA1453; -.
DR   HOGENOM; CLU_009301_11_5_6; -.
DR   InParanoid; Q9I3P8; -.
DR   OMA; FFAADMR; -.
DR   PhylomeDB; Q9I3P8; -.
DR   BioCyc; PAER208964:G1FZ6-1479-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR   GO; GO:0005047; F:signal recognition particle binding; IBA:GO_Central.
DR   GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR   GO; GO:0006605; P:protein targeting; IBA:GO_Central.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR020006; FlhF.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   PANTHER; PTHR43134:SF3; PTHR43134:SF3; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR03499; FlhF; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum biogenesis; Bacterial flagellum protein export;
KW   Cell membrane; GTP-binding; Membrane; Nucleotide-binding;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..429
FT                   /note="Flagellar biosynthesis protein FlhF"
FT                   /id="PRO_0000287816"
FT   BINDING         216..223
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         294..298
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         352..355
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   429 AA;  46499 MW;  4A0CE6B876A6D403 CRC64;
     MQVKRFFAAD MRQAMKLVRD ELGPDAAILG NRRVAGGIEL TAALDYQAPP APSKPNPALE
     VELRKTQARI AEARAELTAR PAETQRKDRQ LFAEEKPARP TLAESMTAAH AKPQVGGQTL
     EAMRFELNGL RELIEVQLGS IAWGQLQHQR PQQANLWRRL QRMGLPAELS KPLLERVAAV
     GDTRQAWRML LAHLSRAVQT PEQDPLDAGG VLALVGPAGA GKTTTLAKMA ARYVLKYGAQ
     SLALVSMDSY RIGAQEQIKT LGRILNVPVT LVDPGQSLIQ ALAPLARKRM VLIDTAGLPA
     SDPALRMQLE ALASPSLNVK NYLVMATTSQ SQVLKSAYQT YRHCGLAGCI LTKLDEAGSL
     GESMALAIAQ RLPVAYLADG PRIPDDLQVA RSHQLVSRAV SLQAPEEPSE DAMAEMFAGL
     YQQPARRAG