ID   FLHF_PSEPU              Reviewed;         437 AA.
AC   O52256;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Flagellar biosynthesis protein FlhF;
DE   AltName: Full=Flagella-associated GTP-binding protein;
GN   Name=flhF;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PRS2000;
RX   PubMed=9503621; DOI=10.1111/j.1574-6968.1998.tb12871.x;
RA   Ditty J.L., Grimm A.C., Harwood C.S.;
RT   "Identification of a chemotaxis gene region from Pseudomonas putida.";
RL   FEMS Microbiol. Lett. 159:267-273(1998).
CC   -!- FUNCTION: Necessary for flagellar biosynthesis. May be involved in
CC       translocation of the flagellum (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. {ECO:0000305}.
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DR   EMBL; AF031898; AAC08059.1; -; Genomic_DNA.
DR   RefSeq; WP_016498564.1; NZ_UGUX01000003.1.
DR   AlphaFoldDB; O52256; -.
DR   SMR; O52256; -.
DR   STRING; 1240350.AMZE01000046_gene250; -.
DR   PRIDE; O52256; -.
DR   GeneID; 45522957; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR020006; FlhF.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   PANTHER; PTHR43134:SF3; PTHR43134:SF3; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR03499; FlhF; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum biogenesis; Bacterial flagellum protein export;
KW   Cell membrane; GTP-binding; Membrane; Nucleotide-binding;
KW   Protein transport; Transport.
FT   CHAIN           1..437
FT                   /note="Flagellar biosynthesis protein FlhF"
FT                   /id="PRO_0000101225"
FT   BINDING         225..232
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         303..307
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         361..364
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   437 AA;  47513 MW;  EBF1A6A650B9B27A CRC64;
     MQVKRFFAAD MRQAMKLVRD ELGADAAIIG NRRIAGGVEL TAALDYKLSA LAPRVPNAEL
     EEELRKTHTR IATAQAELDH RQDSSDNNRQ LFAGQSLTAA EPLIEPHVDA PEAAAAPAPA
     AAPVDPRLFD AMRSELSGLR ELLEVQLGSL AWSQLQGSKP QQANLWRRLQ RIGLSGPIAR
     ELLDLTAEIE EPRQAWRMLL AHLARMIDIP EIEPIEEGGV IAMVGPAGMG KTTTLAKLAA
     RYVLKYGAQN LALVSMDSFR IGAQEQLKTL GRILNVPVTY VDPGQSLAAA LEPLLRKRVV
     LIDTAGLQAS DPALRMQLET LAGRGIAAKN YLVLATTSQK QVLTAAYHSY KRCGLAGCIL
     TKLDETASLG DVLSLAISHE LPVAYLTDGP RIPDDLHLPR GHQLVSRAVN VQQQDEPSEE
     AMADMFADLY HNPRRAG