FLI1_MOUSE
ID FLI1_MOUSE Reviewed; 452 AA.
AC P26323;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Friend leukemia integration 1 transcription factor;
DE AltName: Full=Retroviral integration site protein Fli-1;
GN Name=Fli1; Synonyms=Fli-1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INVOLVEMENT IN ERYTHROLEUKEMIA.
RC STRAIN=BALB/cJ; TISSUE=Spleen;
RX PubMed=2044959; DOI=10.1101/gad.5.6.908;
RA Ben-David Y., Giddens E.B., Letwin K., Bernstein A.;
RT "Erythroleukemia induction by Friend murine leukemia virus: insertional
RT activation of a new member of the ets gene family, Fli-1, closely linked to
RT c-ets-1.";
RL Genes Dev. 5:908-918(1991).
RN [2]
RP CHARACTERIZATION.
RX PubMed=8502483;
RA Zhang L., Lemarchandel V., Romeo P.-H., Ben-David Y., Greer P.,
RA Bernstein A.;
RT "The Fli-1 proto-oncogene, involved in erythroleukemia and Ewing's sarcoma,
RT encodes a transcriptional activator with DNA-binding specificities distinct
RT from other Ets family members.";
RL Oncogene 8:1621-1630(1993).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Sequence-specific transcriptional activator. Recognizes the
CC DNA sequence 5'-C[CA]GGAAGT-3'. {ECO:0000250|UniProtKB:Q01543}.
CC -!- SUBUNIT: Can form homodimers or heterodimers with ETV6/TEL1.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q01543}.
CC -!- TISSUE SPECIFICITY: Expressed in both hematopoietic and
CC nonhematopoietic tissues.
CC -!- INDUCTION: Specifically up-regulated in leukemic clones with F-MuLV
CC insertions up-stream of the FLI-1 locus.
CC -!- DISEASE: Note=Involved in erythroleukemia induction by Friend murine
CC leukemia virus (F-MULV). {ECO:0000269|PubMed:2044959}.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; X59421; CAA42055.1; -; mRNA.
DR CCDS; CCDS40571.1; -.
DR PIR; S17403; S17403.
DR RefSeq; NP_032052.1; NM_008026.5.
DR AlphaFoldDB; P26323; -.
DR BMRB; P26323; -.
DR SMR; P26323; -.
DR BioGRID; 199702; 2.
DR IntAct; P26323; 2.
DR STRING; 10090.ENSMUSP00000016231; -.
DR iPTMnet; P26323; -.
DR PhosphoSitePlus; P26323; -.
DR EPD; P26323; -.
DR jPOST; P26323; -.
DR PaxDb; P26323; -.
DR PeptideAtlas; P26323; -.
DR PRIDE; P26323; -.
DR ProteomicsDB; 267594; -.
DR ABCD; P26323; 85 sequenced antibodies.
DR Antibodypedia; 9350; 698 antibodies from 43 providers.
DR DNASU; 14247; -.
DR Ensembl; ENSMUST00000016231; ENSMUSP00000016231; ENSMUSG00000016087.
DR GeneID; 14247; -.
DR KEGG; mmu:14247; -.
DR UCSC; uc009orz.2; mouse.
DR CTD; 2313; -.
DR MGI; MGI:95554; Fli1.
DR VEuPathDB; HostDB:ENSMUSG00000016087; -.
DR eggNOG; KOG3806; Eukaryota.
DR GeneTree; ENSGT00940000158261; -.
DR HOGENOM; CLU_045216_0_1_1; -.
DR InParanoid; P26323; -.
DR OMA; XSLLAYN; -.
DR OrthoDB; 1113327at2759; -.
DR PhylomeDB; P26323; -.
DR TreeFam; TF350537; -.
DR BioGRID-ORCS; 14247; 6 hits in 77 CRISPR screens.
DR ChiTaRS; Fli1; mouse.
DR PRO; PR:P26323; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; P26323; protein.
DR Bgee; ENSMUSG00000016087; Expressed in peripheral lymph node and 229 other tissues.
DR ExpressionAtlas; P26323; baseline and differential.
DR Genevisible; P26323; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISO:MGI.
DR GO; GO:0009887; P:animal organ morphogenesis; IMP:MGI.
DR GO; GO:0008015; P:blood circulation; IMP:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0035855; P:megakaryocyte development; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:MGI.
DR CDD; cd08541; SAM_PNT-FLI-1; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR035575; Fli-1.
DR InterPro; IPR003118; Pointed_dom.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR035573; SAM_PNT-FLI-1.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 1.
DR PANTHER; PTHR11849:SF161; PTHR11849:SF161; 1.
DR Pfam; PF00178; Ets; 1.
DR Pfam; PF02198; SAM_PNT; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SMART; SM00251; SAM_PNT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR PROSITE; PS51433; PNT; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Phosphoprotein; Proto-oncogene;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..452
FT /note="Friend leukemia integration 1 transcription factor"
FT /id="PRO_0000204125"
FT DOMAIN 112..198
FT /note="PNT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT DNA_BIND 281..361
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 236..260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 39
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 452 AA; 51002 MW; 1F9A06C6893FE2AB CRC64;
MDGTIKEALS VVSDDQSLFD SAYGAAAHLP KADMTASGSP DYGQPHKINP LPPQQEWINQ
PVRVNVKREY DHMNGSRESP VDCSVSKCNK LVGGGEANPM NYNSYMDEKN GPPPPNMTTN
ERRVIVPADP TLWTQEHVRQ WLEWAIKEYG LMEIDTSFFQ NMDGKELCKM NKEDFLRATS
AYNTEVLLSH LSYLRESSLL AYNTTSHTDQ SSRLNVKEDP SYDSVRRGAW NNNMNSGLNK
SPLLGGSQTM GKNTEQRPQP DPYQILGPTS SRLANPGSGQ IQLWQFLLEL LSDSANASCI
TWEGTNGEFK MTDPDEVARR WGERKSKPNM NYDKLSRALR YYYDKNIMTK VHGKRYAYKF
DFHGIAQALQ PHPTETSMYK YPSDISYMPS YHAHQQKVNF VPSHPSSMPV TSSSFFGAAS
QYWTSPTAGI YPNPSVPRHP NTHVPSHLGS YY
