FLIA_ECO57
ID FLIA_ECO57 Reviewed; 239 AA.
AC P0AEM8; P31804;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=RNA polymerase sigma factor FliA {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=RNA polymerase sigma factor for flagellar operon {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=Sigma F {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=Sigma-28 {ECO:0000255|HAMAP-Rule:MF_00962};
GN Name=fliA {ECO:0000255|HAMAP-Rule:MF_00962};
GN OrderedLocusNames=Z3012, ECs2661;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor controls the expression of flagella-related
CC genes. {ECO:0000255|HAMAP-Rule:MF_00962}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00962}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. FliA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00962}.
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DR EMBL; AE005174; AAG56937.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB36084.1; -; Genomic_DNA.
DR PIR; E85809; E85809.
DR PIR; E90961; E90961.
DR RefSeq; NP_310688.1; NC_002695.1.
DR RefSeq; WP_001087467.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0AEM8; -.
DR SMR; P0AEM8; -.
DR STRING; 155864.EDL933_2929; -.
DR EnsemblBacteria; AAG56937; AAG56937; Z3012.
DR EnsemblBacteria; BAB36084; BAB36084; ECs_2661.
DR GeneID; 66674188; -.
DR GeneID; 914052; -.
DR KEGG; ece:Z3012; -.
DR KEGG; ecs:ECs_2661; -.
DR PATRIC; fig|386585.9.peg.2788; -.
DR eggNOG; COG1191; Bacteria.
DR HOGENOM; CLU_014793_8_1_6; -.
DR OMA; IKFETYA; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00962; Sigma70_FliA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR012845; RNA_pol_sigma_FliA_WhiG.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR028617; Sigma70_FliA.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02479; FliA_WhiG; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..239
FT /note="RNA polymerase sigma factor FliA"
FT /id="PRO_0000093982"
FT DNA_BIND 207..226
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 16..88
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 96..166
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 185..233
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT MOTIF 43..46
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 239 AA; 27521 MW; B2930B44E1F24A33 CRC64;
MNSLYTAEGV MDKHSLWQRY VPLVRHEALR LQVRLPASVE LDDLLQAGGI GLLNAVERYD
ALQGTAFTTY AVQRIRGAML DELRSRDWVP RSVRRNAREV AQAIGQLEQE LGRNATETEV
AERLGIDIAD YRQMLLDTNN SQLFSYDEWR EEHGDSIELV TDDHQRENPL QQLLDSNLRQ
RVMEAIETLP EREKLVLTLY YQEELNLKEI GAVLEVGESR VSQLHSQAIK RLRTKLGKL
