FLIA_PSEAE
ID FLIA_PSEAE Reviewed; 247 AA.
AC P29248;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=RNA polymerase sigma factor FliA {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=RNA polymerase sigma factor for flagellar operon {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=Sigma F {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=Sigma-28 {ECO:0000255|HAMAP-Rule:MF_00962};
GN Name=fliA {ECO:0000255|HAMAP-Rule:MF_00962}; Synonyms=rpoF;
GN OrderedLocusNames=PA1455;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=1560774; DOI=10.1111/j.1365-2958.1992.tb01490.x;
RA Starnbach M.N., Lory S.;
RT "The fliA (rpoF) gene of Pseudomonas aeruginosa encodes an alternative
RT sigma factor required for flagellin synthesis.";
RL Mol. Microbiol. 6:459-469(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor controls the expression of flagella-related
CC genes (By similarity). Required for the flagellin gene (fliC)
CC expression. {ECO:0000255|HAMAP-Rule:MF_00962,
CC ECO:0000269|PubMed:1560774}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00962}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. FliA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00962}.
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DR EMBL; X61231; CAA43548.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04844.1; -; Genomic_DNA.
DR PIR; S20544; S20544.
DR RefSeq; NP_250146.1; NC_002516.2.
DR RefSeq; WP_003083070.1; NZ_QZGE01000005.1.
DR AlphaFoldDB; P29248; -.
DR SMR; P29248; -.
DR STRING; 287.DR97_568; -.
DR PaxDb; P29248; -.
DR PRIDE; P29248; -.
DR DNASU; 881996; -.
DR EnsemblBacteria; AAG04844; AAG04844; PA1455.
DR GeneID; 881996; -.
DR KEGG; pae:PA1455; -.
DR PATRIC; fig|208964.12.peg.1506; -.
DR PseudoCAP; PA1455; -.
DR HOGENOM; CLU_014793_8_1_6; -.
DR InParanoid; P29248; -.
DR OMA; IKFETYA; -.
DR PhylomeDB; P29248; -.
DR BioCyc; PAER208964:G1FZ6-1481-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:2000147; P:positive regulation of cell motility; IMP:PseudoCAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR HAMAP; MF_00962; Sigma70_FliA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR012845; RNA_pol_sigma_FliA_WhiG.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR028617; Sigma70_FliA.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02479; FliA_WhiG; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..247
FT /note="RNA polymerase sigma factor FliA"
FT /id="PRO_0000093986"
FT DNA_BIND 212..231
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 22..94
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 102..171
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 190..238
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT MOTIF 49..52
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 247 AA; 27522 MW; 546B69588ABF2251 CRC64;
MTAASGVRMY SKAQAQNSQE QLIQRYAPLV KRIAYHLLGR LPASVQVEDL MQAGMIGLLE
AAKKYDAGKG ASFETYAGIR IRGAMLDEVR KGDWAPRSVH RNTRMVTDAI RAIEARTGRD
AKDHEVAAEL QLSLEDYYGI LSDTQGSRLY SFDDLLQDGE HGLPEDTSLS HNEPIHGLLD
ERFQAALADA IAKLPERERL VLALYYDEEL NLKEIGEVLG VSESRVSQLH SQCAARLRAR
LADWRSA
