FLIA_SALTY
ID FLIA_SALTY Reviewed; 239 AA.
AC P0A2E8; P17168; P74844;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=RNA polymerase sigma factor FliA {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=RNA polymerase sigma factor for flagellar operon {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=Sigma F {ECO:0000255|HAMAP-Rule:MF_00962};
DE AltName: Full=Sigma-28 {ECO:0000255|HAMAP-Rule:MF_00962};
GN Name=fliA {ECO:0000255|HAMAP-Rule:MF_00962}; OrderedLocusNames=STM1956;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14, AND FUNCTION.
RX PubMed=2196428; DOI=10.1007/bf00261713;
RA Ohnishi K., Kutsukake K., Suzuki H., Iino T.;
RT "Gene fliA encodes an alternative sigma factor specific for flagellar
RT operons in Salmonella typhimurium.";
RL Mol. Gen. Genet. 221:139-147(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=KK1004;
RX PubMed=10411266; DOI=10.1099/13500872-145-6-1389;
RA Ikebe T., Iyoda S., Kutsukake K.;
RT "Structure and expression of the fliA operon of Salmonella typhimurium.";
RL Microbiology 145:1389-1396(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
RC STRAIN=168-94;
RX PubMed=9168604; DOI=10.1099/00221287-143-5-1539;
RA Burnens A.P., Stanley J., Sack R., Hunziker P., Brodard I., Nicolet J.;
RT "The flagellin N-methylase gene fliB and an adjacent serovar-specific IS200
RT element in Salmonella typhimurium.";
RL Microbiology 143:1539-1547(1997).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor controls the expression of flagella-related
CC genes. May regulate the expression of genes involved in virulence.
CC {ECO:0000255|HAMAP-Rule:MF_00962, ECO:0000269|PubMed:2196428}.
CC -!- INTERACTION:
CC P0A2E8; P26477: flgM; NbExp=4; IntAct=EBI-10687707, EBI-10687647;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00962}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. FliA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00962}.
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DR EMBL; D00497; BAA00389.1; -; Genomic_DNA.
DR EMBL; AB010947; BAA36397.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20868.1; -; Genomic_DNA.
DR EMBL; Z67749; CAA91563.1; -; Genomic_DNA.
DR PIR; S11895; S11895.
DR RefSeq; NP_460909.1; NC_003197.2.
DR RefSeq; WP_001087453.1; NC_003197.2.
DR AlphaFoldDB; P0A2E8; -.
DR SMR; P0A2E8; -.
DR IntAct; P0A2E8; 1.
DR STRING; 99287.STM1956; -.
DR PaxDb; P0A2E8; -.
DR EnsemblBacteria; AAL20868; AAL20868; STM1956.
DR GeneID; 1253477; -.
DR KEGG; stm:STM1956; -.
DR PATRIC; fig|99287.12.peg.2071; -.
DR HOGENOM; CLU_014793_8_1_6; -.
DR OMA; IKFETYA; -.
DR PhylomeDB; P0A2E8; -.
DR BioCyc; SENT99287:STM1956-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR HAMAP; MF_00962; Sigma70_FliA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR012845; RNA_pol_sigma_FliA_WhiG.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR028617; Sigma70_FliA.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02479; FliA_WhiG; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; DNA-binding; Reference proteome;
KW Sigma factor; Transcription; Transcription regulation; Virulence.
FT CHAIN 1..239
FT /note="RNA polymerase sigma factor FliA"
FT /id="PRO_0000093985"
FT DNA_BIND 207..226
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 16..88
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 96..166
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT REGION 185..233
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT MOTIF 43..46
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 239 AA; 27473 MW; 1667E11EA4251F72 CRC64;
MNSLYTAEGV MDKHSLWQRY VPLVRHEALR LQVRLPASVE LDDLLQAGGI GLLNAVDRYD
ALQGTAFTTY AVQRIRGAML DELRSRDWVP RSVRRNAREV AQAMGQLEQE LGRNATETEV
AERLGIPVAE YRQMLLDTNN SQLFSYDEWR EEHGDSIELV TEEHQQENPL HQLLEGDLRQ
RVMDAIESLP EREQLVLTLY YQEELNLKEI GAVLEVGESR VSQLHSQAIK RLRTKLGKL
