ID   FLIA_YEREN              Reviewed;         230 AA.
AC   P52621;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=RNA polymerase sigma factor FliA {ECO:0000255|HAMAP-Rule:MF_00962};
DE   AltName: Full=RNA polymerase sigma factor for flagellar operon {ECO:0000255|HAMAP-Rule:MF_00962};
DE   AltName: Full=Sigma F {ECO:0000255|HAMAP-Rule:MF_00962};
DE   AltName: Full=Sigma-28 {ECO:0000255|HAMAP-Rule:MF_00962};
GN   Name=fliA {ECO:0000255|HAMAP-Rule:MF_00962};
OS   Yersinia enterocolitica.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W1024 / Serotype O:9;
RX   PubMed=7730257; DOI=10.1128/jb.177.9.2299-2304.1995;
RA   Iriarte M., Stainier I., Mikulskis A., Cornelis G.R.;
RT   "The fliA gene encoding sigma 28 in Yersinia enterocolitica.";
RL   J. Bacteriol. 177:2299-2304(1995).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor controls the expression of flagella-related
CC       genes. {ECO:0000255|HAMAP-Rule:MF_00962}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00962}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. FliA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00962}.
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DR   EMBL; U17393; AAC43394.1; -; Genomic_DNA.
DR   AlphaFoldDB; P52621; -.
DR   SMR; P52621; -.
DR   STRING; 1443113.LC20_02197; -.
DR   eggNOG; COG1191; Bacteria.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00962; Sigma70_FliA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR012845; RNA_pol_sigma_FliA_WhiG.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR028617; Sigma70_FliA.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02479; FliA_WhiG; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..230
FT                   /note="RNA polymerase sigma factor FliA"
FT                   /id="PRO_0000093987"
FT   DNA_BIND        197..216
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT   REGION          6..78
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT   REGION          86..156
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT   REGION          175..223
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00962"
FT   MOTIF           33..36
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   230 AA;  26580 MW;  F527F11014925F23 CRC64;
     MDKNSLWQRY VPLVRHEALR LQVRLPASVE LDDLLQAGGI GLLNAVERYD RLQGTAFTTY
     AVQRIRGAML DELRSRDWAP RSVRRNAREV ASAMQKVEQR LGRPATEQEV AQNLDIDLTE
     YRQILLDTNN SQLFSYDEWR EEHGESVEPM LEGHEDANPL QHLLEGNLRQ RVIEAIEALP
     EREKMVLTLY YQEELNLKEI GAVLEVGESR VSQLHSQAIK RLRARLNNDS