ID   FLICA_PSEAI             Reviewed;         394 AA.
AC   P21184; O30388; O33928; O68382;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=A-type flagellin;
GN   Name=fliC; Synonyms=flaA;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-6.
RC   STRAIN=PAK;
RX   PubMed=2123866; DOI=10.1128/jb.172.12.7188-7199.1990;
RA   Totten P.A., Lory S.;
RT   "Characterization of the type a flagellin gene from Pseudomonas aeruginosa
RT   PAK.";
RL   J. Bacteriol. 172:7188-7199(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-390.
RC   STRAIN=ATCC 27853 / DSM 1117 / CIP 76.110 / JCM 6119 / LMG 6395 / NCIMB
RC   12469;
RX   PubMed=12501433; DOI=10.2323/jgam.44.231;
RA   Tungpradabkul S., Senapin S., Panyim S.;
RT   "PCR-based method for isolation of the flagellin genes from Pseudomonas
RT   species.";
RL   J. Gen. Appl. Microbiol. 44:231-234(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 47-386, AND GLYCOSYLATION.
RC   STRAIN=170018, and 5939;
RX   PubMed=9620973; DOI=10.1128/jb.180.12.3209-3217.1998;
RA   Brimer C.D., Montie T.C.;
RT   "Cloning and comparison of fliC genes and identification of glycosylation
RT   in the flagellin of Pseudomonas aeruginosa a-type strains.";
RL   J. Bacteriol. 180:3209-3217(1998).
RN   [4]
RP   PHOSPHORYLATION.
RC   STRAIN=170018, 2993, 5940, and PAK;
RX   PubMed=7682214; DOI=10.1128/jb.175.8.2458-2461.1993;
RA   Kelly-Wintenberg K., South S.L., Montie T.C.;
RT   "Tyrosine phosphate in a- and b-type flagellins of Pseudomonas
RT   aeruginosa.";
RL   J. Bacteriol. 175:2458-2461(1993).
CC   -!- FUNCTION: Flagellin is the subunit protein which polymerizes to form
CC       the filaments of bacterial flagella.
CC   -!- INTERACTION:
CC       P21184; Q94F62: BAK1; Xeno; NbExp=5; IntAct=EBI-16077660, EBI-617138;
CC       P21184; Q9FL28: FLS2; Xeno; NbExp=5; IntAct=EBI-16077660, EBI-1799448;
CC   -!- SUBCELLULAR LOCATION: Secreted. Bacterial flagellum.
CC   -!- PTM: Phosphorylated on tyrosine residue(s).
CC       {ECO:0000269|PubMed:7682214}.
CC   -!- PTM: Flagellin from strain 5939 but not from strain 170018 is
CC       glycosylated. {ECO:0000269|PubMed:9620973}.
CC   -!- SIMILARITY: Belongs to the bacterial flagellin family. {ECO:0000305}.
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DR   EMBL; M57501; AAA63458.1; -; Genomic_DNA.
DR   EMBL; AF034764; AAC63946.1; -; Genomic_DNA.
DR   EMBL; U76543; AAB66595.1; -; Genomic_DNA.
DR   EMBL; AF003905; AAB66597.1; -; Genomic_DNA.
DR   PIR; A37853; A37853.
DR   RefSeq; WP_003120600.1; NZ_WUDO01000010.1.
DR   AlphaFoldDB; P21184; -.
DR   SMR; P21184; -.
DR   DIP; DIP-61729N; -.
DR   IntAct; P21184; 2.
DR   PRIDE; P21184; -.
DR   eggNOG; COG1344; Bacteria.
DR   GO; GO:0009288; C:bacterial-type flagellum; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   Gene3D; 6.10.10.10; -; 1.
DR   InterPro; IPR001492; Flagellin.
DR   InterPro; IPR046358; Flagellin_C.
DR   InterPro; IPR042187; Flagellin_C_sub2.
DR   InterPro; IPR001029; Flagellin_N.
DR   PANTHER; PTHR42792; PTHR42792; 1.
DR   Pfam; PF00700; Flagellin_C; 1.
DR   Pfam; PF00669; Flagellin_N; 1.
DR   PRINTS; PR00207; FLAGELLIN.
PE   1: Evidence at protein level;
KW   Bacterial flagellum; Direct protein sequencing; Glycoprotein;
KW   Phosphoprotein; Secreted.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2123866"
FT   CHAIN           2..394
FT                   /note="A-type flagellin"
FT                   /id="PRO_0000182619"
FT   VARIANT         116
FT                   /note="V -> A (in strain: PAK)"
FT   VARIANT         280
FT                   /note="T -> A (in strain: PAK and 170018)"
FT   VARIANT         332
FT                   /note="F -> I (in strain: 5939)"
FT   CONFLICT        303..313
FT                   /note="GAQSAVLVIDE -> ALSRRAGDRTT (in Ref. 1; AAA63458)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        321..326
FT                   /note="QRADLG -> SVPTSV (in Ref. 1; AAA63458)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   394 AA;  40066 MW;  A6F08594E45C2E29 CRC64;
     MALTVNTNIA SLNTQRNLNN SSASLNTSLQ RLSTGSRINS AKDDAAGLQI ANRLTSQVNG
     LNVATKNAND GISLAQTAEG ALQQSTNILQ RMRDLSLQSA NGSNSDSERT ALNGEVKQLQ
     KELDRISNTT TFGGRKLLDG SFGVASFQVG SAANEIISVG IDEMSAESLN GTYFKADGGG
     AVTAATASGT VDIAIGITGG SAVNVKVDMK GNETAEQAAA KIAAAVNDAN VGIGAFSDGD
     TISYVSKAGK DGSGAITSAV SGVVIADTGS TGVGTAAGVT PSATAFAKTN DTVAKIDIST
     AKGAQSAVLV IDEAIKQIDA QRADLGAVQN RFDNTINNLK NIGENVSAAR GRIEDTDFAA
     ETANLTKNQV LQQAGTAILA QANQLPQSVL SLLR