FLID_ECOLI
ID FLID_ECOLI Reviewed; 468 AA.
AC P24216; P94745;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 5.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Flagellar hook-associated protein 2;
DE Short=HAP2;
DE AltName: Full=Filament cap protein;
DE AltName: Full=Flagellar cap protein;
GN Name=fliD; Synonyms=flaV, flbC; OrderedLocusNames=b1924, JW1909;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JA11;
RX PubMed=1527488; DOI=10.1099/00221287-138-6-1051;
RA Kawagishi I., Mueller V., Williams A.W., Irikura V.M., Macnab R.M.;
RT "Subdivision of flagellar region III of the Escherichia coli and Salmonella
RT typhimurium chromosomes and identification of two additional flagellar
RT genes.";
RL J. Gen. Microbiol. 138:1051-1065(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-153.
RC STRAIN=K12;
RX PubMed=2659972; DOI=10.1007/bf00332229;
RA Hanafusa T., Sakai A., Tominaga A., Enomoto M.;
RT "Isolation and characterization of Escherichia coli hag operator mutants
RT whose hag48 expression has become repressible by a Salmonella H1
RT repressor.";
RL Mol. Gen. Genet. 216:44-50(1989).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
RX PubMed=6305924; DOI=10.1128/jb.155.1.74-81.1983;
RA Szekely E., Simon M.;
RT "DNA sequence adjacent to flagellar genes and evolution of flagellar-phase
RT variation.";
RL J. Bacteriol. 155:74-81(1983).
CC -!- FUNCTION: Required for the morphogenesis and for the elongation of the
CC flagellar filament by facilitating polymerization of the flagellin
CC monomers at the tip of growing filament. Forms a capping structure,
CC which prevents flagellin subunits (transported through the central
CC channel of the flagellum) from leaking out without polymerization at
CC the distal end.
CC -!- SUBUNIT: Homopentamer. {ECO:0000305}.
CC -!- INTERACTION:
CC P24216; P0ABY2: fliT; NbExp=4; IntAct=EBI-1126352, EBI-1114780;
CC -!- SUBCELLULAR LOCATION: Secreted. Bacterial flagellum.
CC -!- SIMILARITY: Belongs to the FliD family. {ECO:0000305}.
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DR EMBL; M85240; AAA23790.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74991.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15752.1; -; Genomic_DNA.
DR EMBL; X17440; CAA35487.1; -; Genomic_DNA.
DR EMBL; J01607; AAA92490.1; -; Genomic_DNA.
DR PIR; A64956; A64956.
DR RefSeq; NP_416434.1; NC_000913.3.
DR RefSeq; WP_000146784.1; NZ_LN832404.1.
DR PDB; 5H5V; X-ray; 3.00 A; A/B/C/D/E/F=43-416.
DR PDB; 5H5W; X-ray; 2.15 A; A/B=71-267.
DR PDBsum; 5H5V; -.
DR PDBsum; 5H5W; -.
DR AlphaFoldDB; P24216; -.
DR SMR; P24216; -.
DR BioGRID; 4260370; 8.
DR BioGRID; 850785; 1.
DR IntAct; P24216; 8.
DR STRING; 511145.b1924; -.
DR SWISS-2DPAGE; P24216; -.
DR PaxDb; P24216; -.
DR PRIDE; P24216; -.
DR DNASU; 946428; -.
DR EnsemblBacteria; AAC74991; AAC74991; b1924.
DR EnsemblBacteria; BAA15752; BAA15752; BAA15752.
DR GeneID; 946428; -.
DR KEGG; ecj:JW1909; -.
DR KEGG; eco:b1924; -.
DR PATRIC; fig|1411691.4.peg.325; -.
DR EchoBASE; EB0834; -.
DR eggNOG; COG1345; Bacteria.
DR InParanoid; P24216; -.
DR OMA; TLEWQRD; -.
DR PhylomeDB; P24216; -.
DR BioCyc; EcoCyc:EG10841-MON; -.
DR PHI-base; PHI:6733; -.
DR PRO; PR:P24216; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009421; C:bacterial-type flagellum filament cap; IBA:GO_Central.
DR GO; GO:0009424; C:bacterial-type flagellum hook; IEA:InterPro.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0071973; P:bacterial-type flagellum-dependent cell motility; IMP:EcoCyc.
DR GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR InterPro; IPR040026; FliD.
DR InterPro; IPR010809; FliD_C.
DR InterPro; IPR003481; FliD_N.
DR PANTHER; PTHR30288; PTHR30288; 1.
DR Pfam; PF07195; FliD_C; 1.
DR Pfam; PF02465; FliD_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacterial flagellum; Coiled coil; Reference proteome;
KW Secreted.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..468
FT /note="Flagellar hook-associated protein 2"
FT /id="PRO_0000177017"
FT COILED 411..439
FT /evidence="ECO:0000255"
FT CONFLICT 114
FT /note="R -> T (in Ref. 1; AAA23790)"
FT /evidence="ECO:0000305"
FT HELIX 45..64
FT /evidence="ECO:0007829|PDB:5H5V"
FT HELIX 67..71
FT /evidence="ECO:0007829|PDB:5H5V"
FT STRAND 73..78
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 93..101
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 107..112
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 126..133
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 139..143
FT /evidence="ECO:0007829|PDB:5H5W"
FT HELIX 145..147
FT /evidence="ECO:0007829|PDB:5H5V"
FT HELIX 150..160
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 163..172
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 175..184
FT /evidence="ECO:0007829|PDB:5H5W"
FT HELIX 187..189
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 191..197
FT /evidence="ECO:0007829|PDB:5H5W"
FT HELIX 199..205
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 211..213
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 215..220
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 225..229
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 232..237
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 239..245
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 248..252
FT /evidence="ECO:0007829|PDB:5H5W"
FT STRAND 261..266
FT /evidence="ECO:0007829|PDB:5H5W"
FT HELIX 269..293
FT /evidence="ECO:0007829|PDB:5H5V"
FT HELIX 316..330
FT /evidence="ECO:0007829|PDB:5H5V"
FT STRAND 335..337
FT /evidence="ECO:0007829|PDB:5H5V"
FT HELIX 341..344
FT /evidence="ECO:0007829|PDB:5H5V"
FT STRAND 346..348
FT /evidence="ECO:0007829|PDB:5H5V"
FT TURN 350..352
FT /evidence="ECO:0007829|PDB:5H5V"
FT STRAND 355..357
FT /evidence="ECO:0007829|PDB:5H5V"
FT HELIX 359..377
FT /evidence="ECO:0007829|PDB:5H5V"
FT TURN 378..380
FT /evidence="ECO:0007829|PDB:5H5V"
FT STRAND 381..383
FT /evidence="ECO:0007829|PDB:5H5V"
FT HELIX 386..398
FT /evidence="ECO:0007829|PDB:5H5V"
FT HELIX 403..410
FT /evidence="ECO:0007829|PDB:5H5V"
SQ SEQUENCE 468 AA; 48456 MW; 5C16E5B8DFBB8661 CRC64;
MASISSLGVG SGLDLSSILD SLTAAQKATL TPISNQQSSF TAKLSAYGTL KSALTTFQTA
NTALSKADLF SATSTTSSTT AFSATTAGNA IAGKYTISVT HLAQAQTLTT RTTRDDTKTA
IATSDSKLTI QQGGDKDPIT IDISAANSSL SGIRDAINNA KAGVSASIIN VGNGEYRLSV
TSNDTGLDNA MTLSVSGDDA LQSFMGYDAS ASSNGMEVSV AAQNAQLTVN NVAIENSSNT
ISDALENITL NLNDVTTGNQ TLTITQDTSK AQTAIKDWVN AYNSLIDTFS SLTKYTAVDA
GADSQSSSNG ALLGDSTLRT IQTQLKSMLS NTVSSSSYKT LAQIGITTDP SDGKLELDAD
KLTAALKKDA SGVGALIVGD GKKTGITTTI GSNLTSWLST TGIIKAATDG VSKTLNKLTK
DYNAASDRID AQVARYKEQF TQLDVLMTSL NSTSSYLTQQ FENNSNSK
