FLID_HELPY
ID FLID_HELPY Reviewed; 685 AA.
AC P96786;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Flagellar hook-associated protein 2;
DE Short=HAP2;
DE AltName: Full=Filament cap protein;
DE AltName: Full=Flagellar cap protein;
GN Name=fliD; OrderedLocusNames=HP_0752;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16, AND
RP CHARACTERIZATION.
RC STRAIN=KCTC 0217BP;
RX PubMed=10559162; DOI=10.1128/jb.181.22.6969-6976.1999;
RA Kim J.S., Chang J.H., Chung S.I., Yum J.S.;
RT "Molecular cloning and characterization of the Helicobacter pylori fliD
RT gene, an essential factor in flagellar structure and motility.";
RL J. Bacteriol. 181:6969-6976(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
CC -!- FUNCTION: Required for the morphogenesis and for the elongation of the
CC flagellar filament by facilitating polymerization of the flagellin
CC monomers at the tip of growing filament. Forms a capping structure,
CC which prevents flagellin subunits (transported through the central
CC channel of the flagellum) from leaking out without polymerization at
CC the distal end. Essential to colonize and establish infection in
CC gastric mucosa as a result of its essential role in motility. Has
CC effect on flaA gene transcription.
CC -!- SUBUNIT: Homopentamer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted. Bacterial flagellum.
CC -!- SIMILARITY: Belongs to the FliD family. {ECO:0000305}.
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DR EMBL; U82981; AAC35999.1; -; Genomic_DNA.
DR EMBL; AE000511; AAD07800.1; -; Genomic_DNA.
DR PIR; H64613; H64613.
DR RefSeq; NP_207545.1; NC_000915.1.
DR PDB; 6IWY; X-ray; 2.60 A; A=75-480.
DR PDBsum; 6IWY; -.
DR AlphaFoldDB; P96786; -.
DR SMR; P96786; -.
DR DIP; DIP-3401N; -.
DR IntAct; P96786; 1.
DR MINT; P96786; -.
DR STRING; 85962.C694_03870; -.
DR PaxDb; P96786; -.
DR EnsemblBacteria; AAD07800; AAD07800; HP_0752.
DR KEGG; hpy:HP_0752; -.
DR PATRIC; fig|85962.8.peg.785; -.
DR eggNOG; COG1345; Bacteria.
DR OMA; IMAERFA; -.
DR PhylomeDB; P96786; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0009288; C:bacterial-type flagellum; IMP:CACAO.
DR GO; GO:0009421; C:bacterial-type flagellum filament cap; IBA:GO_Central.
DR GO; GO:0009424; C:bacterial-type flagellum hook; IEA:InterPro.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0071973; P:bacterial-type flagellum-dependent cell motility; IBA:GO_Central.
DR GO; GO:0071978; P:bacterial-type flagellum-dependent swarming motility; IMP:CACAO.
DR GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR InterPro; IPR040026; FliD.
DR InterPro; IPR010809; FliD_C.
DR InterPro; IPR003481; FliD_N.
DR PANTHER; PTHR30288; PTHR30288; 1.
DR Pfam; PF07195; FliD_C; 1.
DR Pfam; PF02465; FliD_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacterial flagellum; Coiled coil; Direct protein sequencing;
KW Reference proteome; Secreted.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:10559162"
FT CHAIN 2..685
FT /note="Flagellar hook-associated protein 2"
FT /id="PRO_0000177019"
FT COILED 618..647
FT /evidence="ECO:0000255"
FT VARIANT 59
FT /note="A -> S (in strain: KCTC 0217BP)"
FT VARIANT 86
FT /note="S -> N (in strain: KCTC 0217BP)"
FT VARIANT 91
FT /note="V -> A (in strain: KCTC 0217BP)"
FT VARIANT 193
FT /note="V -> I (in strain: KCTC 0217BP)"
FT VARIANT 231
FT /note="N -> S (in strain: KCTC 0217BP)"
FT VARIANT 258
FT /note="M -> I (in strain: KCTC 0217BP)"
FT VARIANT 270
FT /note="N -> D (in strain: KCTC 0217BP)"
FT VARIANT 363
FT /note="I -> V (in strain: KCTC 0217BP)"
FT VARIANT 368
FT /note="A -> S (in strain: KCTC 0217BP)"
FT VARIANT 373
FT /note="S -> N (in strain: KCTC 0217BP)"
FT VARIANT 422
FT /note="N -> G (in strain: KCTC 0217BP)"
FT CONFLICT 654..685
FT /note="ERFAAYDSQISKANQKFNSVQMMIDQAAAKKN -> DVLPLMIAKSLKPIKN
FT SIPCK (in Ref. 2; AAD07800)"
FT /evidence="ECO:0000305"
FT STRAND 82..88
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 98..104
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 110..118
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 122..124
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 126..135
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 138..144
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 150..160
FT /evidence="ECO:0007829|PDB:6IWY"
FT TURN 161..163
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 164..171
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 173..176
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 178..186
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 189..191
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 194..200
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 209..211
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 219..226
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 230..236
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 239..241
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 247..264
FT /evidence="ECO:0007829|PDB:6IWY"
FT TURN 266..268
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 269..273
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 276..282
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 283..285
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 287..292
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 298..302
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 305..308
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 320..322
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 336..338
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 360..367
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 372..376
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 382..386
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 389..397
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 398..403
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 404..407
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 411..415
FT /evidence="ECO:0007829|PDB:6IWY"
FT HELIX 417..424
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 427..431
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 436..439
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 446..451
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 460..463
FT /evidence="ECO:0007829|PDB:6IWY"
FT STRAND 473..475
FT /evidence="ECO:0007829|PDB:6IWY"
SQ SEQUENCE 685 AA; 74131 MW; 12D4F56473D047F6 CRC64;
MAIGSLSSLG LGSKVLNYDV IDKLKDADEK ALIAPLDKKM EQNVEKQKAL VEIKTLLSAL
KGPVKTLSDY STYISRKSNV TGDALSASVG VGVPIQDIKV DVQNLAQGDI NELGAKFSSR
DDIFSQVDTT LKFYTQNKDY AVNIKAGMTL GDVAQSITDA TNGEVMGIVM KTGGNDPYQL
MVNTKNTGED NRVYFGSHLQ STLTNKNALS LGVDGSGKSE VSLNLKGADG NMHEVPIMLE
LPESASIKQK NTAIQKAMEQ ALENDPNFKN LIANGDISID TLHGGESLII NDRRGGNIEV
KGSKAKELGF LQTTTQESDL LKSSRTIKEG KLEGVVSLNG QKLDLSALTK ESNTSEENTD
AIIQAINAKE GLSAFKNAEG KLVINSKTGM LTIKGEDALG KASLKDLGLN AGMVQSYEAS
QNTLFMSKNL QKASDSAFTY NGVSITRPTN EVNDVISGVN ITLEQTTEPN KPAIISVSRD
NQAIIDSLTE FVKAYNELIP KLDEDTRYDA DTKIAGIFNG VGDIRAIRSS LNNVFSYSVH
TDNGVESLMK YGLSLDDKGV MSLDEAKLSS ALNSNPKATQ DFFYGSDSKD MGGREIHQEG
IFSKFNQVIA NLIDGGNAKL KIYEDSLDRD AKSLTKDKEN AQELLKTRYN IMAERFAAYD
SQISKANQKF NSVQMMIDQA AAKKN
