ID   FLIF_BACSU              Reviewed;         536 AA.
AC   P23447;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 2.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Flagellar M-ring protein;
GN   Name=fliF; OrderedLocusNames=BSU16210;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1905667; DOI=10.1016/0378-1119(91)90220-6;
RA   Zuberi A.R., Ying C., Bischoff D.S., Ordal G.W.;
RT   "Gene-protein relationships in the flagellar hook-basal body complex of
RT   Bacillus subtilis: sequences of the flgB, flgC, flgG, fliE and fliF
RT   genes.";
RL   Gene 101:23-31(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-536.
RC   STRAIN=168;
RX   PubMed=1828465; DOI=10.1128/jb.173.11.3573-3579.1991;
RA   Albertini A.M., Caramori T., Crabb W.D., Scoffone F., Galizzi A.;
RT   "The flaA locus of Bacillus subtilis is part of a large operon coding for
RT   flagellar structures, motility functions, and an ATPase-like polypeptide.";
RL   J. Bacteriol. 173:3573-3579(1991).
CC   -!- FUNCTION: The M ring may be actively involved in energy transduction.
CC   -!- SUBUNIT: The basal body constitutes a major portion of the flagellar
CC       organelle and consists of two rings, one in the peptidoglycan layer and
CC       one in the plasma membrane.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}. Bacterial flagellum basal body {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the FliF family. {ECO:0000305}.
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DR   EMBL; M54965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AL009126; CAB13494.1; -; Genomic_DNA.
DR   EMBL; X56049; CAA39520.1; -; Genomic_DNA.
DR   PIR; JG0022; JG0022.
DR   RefSeq; NP_389503.1; NC_000964.3.
DR   RefSeq; WP_010886506.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P23447; -.
DR   SMR; P23447; -.
DR   IntAct; P23447; 1.
DR   STRING; 224308.BSU16210; -.
DR   jPOST; P23447; -.
DR   PaxDb; P23447; -.
DR   PRIDE; P23447; -.
DR   EnsemblBacteria; CAB13494; CAB13494; BSU_16210.
DR   GeneID; 936677; -.
DR   KEGG; bsu:BSU16210; -.
DR   PATRIC; fig|224308.43.peg.1715; -.
DR   eggNOG; COG1766; Bacteria.
DR   InParanoid; P23447; -.
DR   OMA; NYEVNRI; -.
DR   PhylomeDB; P23447; -.
DR   BioCyc; BSUB:BSU16210-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0009431; C:bacterial-type flagellum basal body, MS ring; IEA:InterPro.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003774; F:cytoskeletal motor activity; IEA:InterPro.
DR   GO; GO:0044780; P:bacterial-type flagellum assembly; IMP:CACAO.
DR   GO; GO:0071978; P:bacterial-type flagellum-dependent swarming motility; IMP:CACAO.
DR   Gene3D; 3.30.300.30; -; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR013556; Flag_M-ring_C.
DR   InterPro; IPR000067; FlgMring_FliF.
DR   InterPro; IPR006182; FliF_N_dom.
DR   InterPro; IPR043427; YscJ/FliF.
DR   PANTHER; PTHR30046; PTHR30046; 1.
DR   PANTHER; PTHR30046:SF0; PTHR30046:SF0; 1.
DR   Pfam; PF01514; YscJ_FliF; 1.
DR   Pfam; PF08345; YscJ_FliF_C; 1.
DR   PIRSF; PIRSF004862; FliF; 1.
DR   PRINTS; PR01009; FLGMRINGFLIF.
DR   TIGRFAMs; TIGR00206; fliF; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..536
FT                   /note="Flagellar M-ring protein"
FT                   /id="PRO_0000180873"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        451..471
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          308..342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   536 AA;  59300 MW;  21110D4C7CF1927A CRC64;
     MNRTLMQMKN KTSEFWKNRS KLQKILMVSA LAAIIIIGII ISVFASNSKM APLYKDLSAE
     EAGQIKEELD AKKVPNELSN GGTVISVPED QVDSLKVQMA AEGLPKTGSI DYSFFGQNAG
     FGLTDNEFDM VKVKATQTEL SNLINEMDGI KNSKVMINLP KDAVFVGEEQ SAASASIVLQ
     IQPGYTLDQS QINGLYHLVS KSVPNLKEDN IVIMDQNSTY YDKSDSDAGS YADSYSSQQG
     IKSQVEKDIQ KHVQSLLGTM MGQDKVVVSV TADIDFTKEN RTEDIVEPVD KENMEGIAVS
     AEKVSETYQG DGAANGGTAG TGEEDVTNYK ADGENTESGN YEKNSNKINY EVNRIHKEIA
     ESPYKVRDLG IQVMVEPPDA KNTASLSTER QDDIQKILST VVRTSLDKDE TQNQNLSDAD
     INNKIVVSVQ PFDGKVNLDT NTEESSGIPL WAYIVGGVLI AAIIVLIIML IRKKRAQEDE
     FEEYEYEVPQ EPINLPDINE EENETAESVR RKQLEKMAKD KPEDFAKLLR SWLAED