FLIF_BRUAB
ID FLIF_BRUAB Reviewed; 580 AA.
AC O52069; Q576H8;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 3.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Flagellar M-ring protein;
GN Name=fliF; OrderedLocusNames=BruAb2_1083;
OS Brucella abortus biovar 1 (strain 9-941).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=262698;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=544 / Biovar 1;
RX PubMed=11013709; DOI=10.1089/omi.1.1998.3.21;
RA Halling S.M.;
RT "On the presence and organization of open reading frames of the nonmotile
RT pathogen Brucella abortus similar to class II, III, and IV flagellar genes
RT and to LcrD virulence superfamily.";
RL Microb. Comp. Genomics 3:21-29(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=9-941;
RX PubMed=15805518; DOI=10.1128/jb.187.8.2715-2726.2005;
RA Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z.,
RA Li L.-L., Kapur V., Alt D.P., Olsen S.C.;
RT "Completion of the genome sequence of Brucella abortus and comparison to
RT the highly similar genomes of Brucella melitensis and Brucella suis.";
RL J. Bacteriol. 187:2715-2726(2005).
CC -!- FUNCTION: The M ring may be actively involved in energy transduction.
CC {ECO:0000250}.
CC -!- SUBUNIT: The basal body constitutes a major portion of the flagellar
CC organelle and consists of five rings (E,L,P,S, and M) mounted on a
CC central rod. The M ring is integral to the inner membrane of the cell
CC and may be connected to the flagellar rod via the S ring. The S
CC (supramembrane ring) lies just distal to the M ring. The L and P rings
CC lie in the outer membrane and the periplasmic space, respectively (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Bacterial flagellum basal body
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the FliF family. {ECO:0000305}.
CC -!- CAUTION: Brucella species display species-specific inactivation of
CC flagellar genes and are consequently nonmotile. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC01568.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF019251; AAC01568.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE017224; AAX76456.1; -; Genomic_DNA.
DR RefSeq; WP_011265437.1; NC_006933.1.
DR AlphaFoldDB; O52069; -.
DR SMR; O52069; -.
DR PRIDE; O52069; -.
DR EnsemblBacteria; AAX76456; AAX76456; BruAb2_1083.
DR KEGG; bmb:BruAb2_1083; -.
DR HOGENOM; CLU_028108_4_0_5; -.
DR OMA; ITTRPHY; -.
DR PRO; PR:O52069; -.
DR Proteomes; UP000000540; Chromosome II.
DR GO; GO:0009431; C:bacterial-type flagellum basal body, MS ring; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003774; F:cytoskeletal motor activity; IEA:InterPro.
DR GO; GO:0071973; P:bacterial-type flagellum-dependent cell motility; IEA:InterPro.
DR Gene3D; 3.30.300.30; -; 1.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR013556; Flag_M-ring_C.
DR InterPro; IPR000067; FlgMring_FliF.
DR InterPro; IPR006182; FliF_N_dom.
DR InterPro; IPR043427; YscJ/FliF.
DR PANTHER; PTHR30046; PTHR30046; 1.
DR PANTHER; PTHR30046:SF0; PTHR30046:SF0; 1.
DR Pfam; PF01514; YscJ_FliF; 1.
DR Pfam; PF08345; YscJ_FliF_C; 1.
DR PIRSF; PIRSF004862; FliF; 1.
DR PRINTS; PR01009; FLGMRINGFLIF.
DR TIGRFAMs; TIGR00206; fliF; 1.
PE 3: Inferred from homology;
KW Bacterial flagellum; Cell inner membrane; Cell membrane; Membrane;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..580
FT /note="Flagellar M-ring protein"
FT /id="PRO_0000180875"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 445..465
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 273..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 275..306
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 86
FT /note="D -> Y (in Ref. 1; AAC01568)"
FT /evidence="ECO:0000305"
FT CONFLICT 336
FT /note="M -> T (in Ref. 1; AAC01568)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 580 AA; 62198 MW; AD6D676BEE3188D3 CRC64;
MAVVWMQQNF QQLIEQLKGT LGKLGARKLI ALGLVGAALM GAILYTSIYL GRPSYETLYV
GLSRDDVNRM GLALGEAGIP FDVKSDGSSI LVPIGKAENA RMYLAEKGLP TSNNAGYELF
DNMGSLGLTS FMQEITRVRA LEGEIARTIQ AIRGVKAARV HIVLAEKGSF RRGDQKPSAS
VVIRAEGGFS AESAQSIRQL VAAAVPSLDA SSVTVLDTNG HLLASAGEGA NGAALMTASL
EQQVASHVDD SIRKALAPYL GLGHFQTSVQ AALDTDRRQT KETTYDPESR VERSVRVVRE
SGDSRNNRND NATGVEQNIP QEQIQNRNGE SSTEKMDRRE ELTNYEVNSK TVSTVSDGYS
IKRLSIAVVI DQARLLQTAG TTPPPANFVD QQITKIRDLV ATAAGLNTNR GDVINVTAVN
FLDPAGADME PVSAPWTDTL LRQSGSYANA LAILAAVGLL IWFGLRPLLR DQNVKPAGTE
VAIREAGEVA TPNFIGGAES VGEGVQAVIG GPAAYADQMK TSLSDLRQRM RMPAKLRLEQ
MIEMDEERVA AVLKQWIHET ASGREADPAK ASAMPELKAA