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AK_PSEAE
ID   AK_PSEAE                Reviewed;         412 AA.
AC   O69077; Q9I552;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2001, sequence version 2.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Aspartokinase;
DE            EC=2.7.2.4;
DE   AltName: Full=Aspartate kinase;
GN   Name=lysC; OrderedLocusNames=PA0904;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 198-412.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RA   Hindle Z., Throup J.P., Francis K.P., Bycroft B.W., Williams P.,
RA   Stewart G.S.A.B.;
RL   Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1-412.
RA   Li C., Yang M., Bao R.;
RT   "Structural of Pseudomonas aeruginosa Lysc.";
RL   Submitted (SEP-2017) to the PDB data bank.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-aspartate = 4-phospho-L-aspartate + ADP;
CC         Xref=Rhea:RHEA:23776, ChEBI:CHEBI:29991, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57535, ChEBI:CHEBI:456216; EC=2.7.2.4;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC       pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; L-homoserine from L-aspartate: step 1/3.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine
CC       from L-aspartate: step 1/5.
CC   -!- SIMILARITY: Belongs to the aspartokinase family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG04293.1; -; Genomic_DNA.
DR   EMBL; AF061757; AAC16241.1; -; Genomic_DNA.
DR   PIR; C83531; C83531.
DR   RefSeq; NP_249595.1; NC_002516.2.
DR   RefSeq; WP_003085971.1; NZ_QZGE01000007.1.
DR   PDB; 5YEI; X-ray; 2.30 A; A/C/E/G=1-412, B/D/F/H=249-412.
DR   PDBsum; 5YEI; -.
DR   AlphaFoldDB; O69077; -.
DR   SMR; O69077; -.
DR   STRING; 287.DR97_1039; -.
DR   PaxDb; O69077; -.
DR   PRIDE; O69077; -.
DR   EnsemblBacteria; AAG04293; AAG04293; PA0904.
DR   GeneID; 878315; -.
DR   KEGG; pae:PA0904; -.
DR   PATRIC; fig|208964.12.peg.939; -.
DR   PseudoCAP; PA0904; -.
DR   HOGENOM; CLU_009116_3_2_6; -.
DR   InParanoid; O69077; -.
DR   OMA; DMIVQTI; -.
DR   PhylomeDB; O69077; -.
DR   BioCyc; PAER208964:G1FZ6-920-MON; -.
DR   BRENDA; 2.7.2.4; 5087.
DR   UniPathway; UPA00034; UER00015.
DR   UniPathway; UPA00050; UER00461.
DR   UniPathway; UPA00051; UER00462.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004072; F:aspartate kinase activity; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0019877; P:diaminopimelate biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009090; P:homoserine biosynthetic process; IBA:GO_Central.
DR   GO; GO:0009089; P:lysine biosynthetic process via diaminopimelate; IBA:GO_Central.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd04261; AAK_AKii-LysC-BS; 1.
DR   Gene3D; 3.40.1160.10; -; 1.
DR   InterPro; IPR036393; AceGlu_kinase-like_sf.
DR   InterPro; IPR045865; ACT-like_dom_sf.
DR   InterPro; IPR002912; ACT_dom.
DR   InterPro; IPR041740; AKii-LysC-BS.
DR   InterPro; IPR001048; Asp/Glu/Uridylate_kinase.
DR   InterPro; IPR005260; Asp_kin_monofn.
DR   InterPro; IPR001341; Asp_kinase.
DR   InterPro; IPR018042; Aspartate_kinase_CS.
DR   InterPro; IPR027795; CASTOR_ACT_dom.
DR   Pfam; PF00696; AA_kinase; 1.
DR   Pfam; PF01842; ACT; 1.
DR   Pfam; PF13840; ACT_7; 1.
DR   PIRSF; PIRSF000726; Asp_kin; 1.
DR   SUPFAM; SSF53633; SSF53633; 1.
DR   SUPFAM; SSF55021; SSF55021; 2.
DR   TIGRFAMs; TIGR00656; asp_kin_monofn; 1.
DR   TIGRFAMs; TIGR00657; asp_kinases; 1.
DR   PROSITE; PS51671; ACT; 2.
DR   PROSITE; PS00324; ASPARTOKINASE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Amino-acid biosynthesis; ATP-binding;
KW   Diaminopimelate biosynthesis; Kinase; Lysine biosynthesis;
KW   Nucleotide-binding; Reference proteome; Repeat; Transferase.
FT   CHAIN           1..412
FT                   /note="Aspartokinase"
FT                   /id="PRO_0000066679"
FT   DOMAIN          266..340
FT                   /note="ACT 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT   DOMAIN          346..412
FT                   /note="ACT 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT   STRAND          3..8
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   TURN            10..13
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           16..31
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          35..40
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           46..55
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           63..70
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           72..87
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          92..95
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           97..100
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          102..104
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          113..116
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           119..126
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          130..134
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          137..139
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          145..147
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           152..163
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          167..177
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          179..181
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   TURN            183..185
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          193..196
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           197..202
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           204..206
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          209..211
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           213..221
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          226..230
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          238..241
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          253..261
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          264..272
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           277..287
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          295..297
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          304..311
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           313..315
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           316..329
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          333..338
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          340..348
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           351..353
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           354..367
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          374..377
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          379..387
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           388..390
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   HELIX           391..401
FT                   /evidence="ECO:0007829|PDB:5YEI"
FT   STRAND          402..404
FT                   /evidence="ECO:0007829|PDB:5YEI"
SQ   SEQUENCE   412 AA;  44389 MW;  A06ACAA5E914F337 CRC64;
     MALIVQKFGG TSVGTVERIE QVAEKVKKFR EAGDDVVVVV SAMSGETNRL IGLANQIMEQ
     PVPRELDVMV STGEQVTIAL LSMALIKRGV PAVSYTGNQV RILTDSAHTK ARILHIDDTH
     IRADLKAGRV VVVAGFQGVD GNGNITTLGR GGSDTTGVAL AAALKADECQ IYTDVDGVYT
     TDPRVVPQAR RLDKITFEEM LEMASLGSKV LQIRAVEFAG KYNVPLRVLH SFQEGPGTLI
     TIDDEEESME QPIISGIAFN RDEAKLTIRG VPDTPGVAFK ILGPISAANV EVDMIVQNVA
     HDNTTDFTFT VHRNDYLNAL EILKQTAANI GAREAIGDTN IAKVSIVGVG MRSHAGVASR
     MFEALAKESI NIQMISTSEI KVSVVIEEKY LELAVRALHT AFELDAPARQ GE
 
 
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