ID   FLIR_ECOLI              Reviewed;         261 AA.
AC   P33135; P76327;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 148.
DE   RecName: Full=Flagellar biosynthetic protein FliR;
GN   Name=fliR; Synonyms=flaP; OrderedLocusNames=b1950, JW1934;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8282695; DOI=10.1128/jb.176.1.189-197.1994;
RA   Malakooti J., Ely B., Matsumura P.;
RT   "Molecular characterization, nucleotide sequence, and expression of the
RT   fliO, fliP, fliQ, and fliR genes of Escherichia coli.";
RL   J. Bacteriol. 176:189-197(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Role in flagellar biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:15919996}. Bacterial flagellum basal body
CC       {ECO:0000269|PubMed:15919996}.
CC   -!- SIMILARITY: Belongs to the FliR/MopE/SpaR family. {ECO:0000305}.
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DR   EMBL; L22182; AAC36861.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75017.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15775.1; -; Genomic_DNA.
DR   PIR; C64959; C64959.
DR   RefSeq; NP_416460.1; NC_000913.3.
DR   RefSeq; WP_000942319.1; NZ_LN832404.1.
DR   AlphaFoldDB; P33135; -.
DR   SMR; P33135; -.
DR   BioGRID; 4261046; 276.
DR   ComplexPortal; CPX-5885; Flagellar export complex.
DR   IntAct; P33135; 4.
DR   STRING; 511145.b1950; -.
DR   PaxDb; P33135; -.
DR   EnsemblBacteria; AAC75017; AAC75017; b1950.
DR   EnsemblBacteria; BAA15775; BAA15775; BAA15775.
DR   GeneID; 946464; -.
DR   KEGG; ecj:JW1934; -.
DR   KEGG; eco:b1950; -.
DR   PATRIC; fig|1411691.4.peg.301; -.
DR   EchoBASE; EB1920; -.
DR   eggNOG; COG1684; Bacteria.
DR   HOGENOM; CLU_063626_4_0_6; -.
DR   InParanoid; P33135; -.
DR   OMA; APQFNIF; -.
DR   PhylomeDB; P33135; -.
DR   BioCyc; EcoCyc:EG11977-MON; -.
DR   PRO; PR:P33135; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009288; C:bacterial-type flagellum; IC:ComplexPortal.
DR   GO; GO:0120102; C:bacterial-type flagellum secretion apparatus; IC:ComplexPortal.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0030257; C:type III protein secretion system complex; IC:ComplexPortal.
DR   GO; GO:0044780; P:bacterial-type flagellum assembly; IEA:InterPro.
DR   GO; GO:0071973; P:bacterial-type flagellum-dependent cell motility; IC:ComplexPortal.
DR   GO; GO:0006935; P:chemotaxis; IC:ComplexPortal.
DR   GO; GO:0030254; P:protein secretion by the type III secretion system; IC:ComplexPortal.
DR   GO; GO:0006605; P:protein targeting; IEA:InterPro.
DR   InterPro; IPR006303; FliR.
DR   InterPro; IPR002010; T3SS_IM_R.
DR   PANTHER; PTHR30065; PTHR30065; 1.
DR   Pfam; PF01311; Bac_export_1; 1.
DR   PRINTS; PR00953; TYPE3IMRPROT.
DR   TIGRFAMs; TIGR01400; fliR; 1.
PE   3: Inferred from homology;
KW   Bacterial flagellum; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..261
FT                   /note="Flagellar biosynthetic protein FliR"
FT                   /id="PRO_0000192055"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        184..204
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        235..255
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        223
FT                   /note="T -> A (in Ref. 1; AAC36861)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   261 AA;  28543 MW;  55405C88D4542B7F CRC64;
     MLQVTSEQWL SWLNLYFWPL LRVLALISTA PILSERSVPK RVKLGLAMMI TFAIAPSLPA
     NDVPVFSFFA LWLAVQQILI GIALGFTMQF AFAAVRTAGE IIGLQMGLSF ATFVDPASHL
     NMPVLARIMD MLALLLFLTF NGHLWLISLL VDTFHTLPIG GEPLNSNAFL ALTKAGSLIF
     LNGLMLALPL ITLLLTLNLA LGLLNRMAPQ LSIFVIGFPL TLTVGISLMA ALMPLIAPFC
     EHLFSEIFNL LADIISELPL I